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Fig. 2 | Molecular Brain

Fig. 2

From: Role of Calcr expressing neurons in the medial amygdala in social contact among females

Fig. 2

Targeted Calcr knockdown in the MeApd does not affect behavioral responses to social isolation. A Timeline of behavioral experiments. At 2 weeks before testing, female mice were administered an AAV expressing shRNA-EGFP for specific Calcr knockdown or with a shRNA virus carrying scrambled shRNA (Cont shRNA) into the MeApd region. An AAV-injected adult female mouse was subjected to a somatic isolation test and reunion tests. B Representative coronal sections of shRNA-EGFP viral expression in the MeApd showing the efficacy of Calcr shRNAs. Scale bars, 2.5 mm (Left), 500 μm (Middle and Right). A shRNA virus carrying scrambled shRNA was used as control. C Quantification of fold changes in the number of Calcr + cells distributed in the MeApd (Cont shRNA: n = 15 mice and Calcr shRNA: n = 12 mice). D Time course of biting responses (Cont shRNA: n = 15 mice and Calcr shRNA: n = 12 mice). E Quantification of the total number of biting responses over 30 min (Cont shRNA: n = 15 mice and Calcr shRNA: n = 12 mice). F Number of various behaviors per min observed under somatic isolation for 30 min (Cont shRNA: n = 15 mice and Calcr shRNA: n = 12 mice). Time bins 15 s (D–F). Asterisks denote significant differences between the two groups (C, E, F: Welch’s unpaired t-test, D: 2-way repeated measure ANOVA tests followed by Sidak's post hoc tests; ***P < 0.001). Graphs represent mean ± SEM. See Addi for detailed statics

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