Fig. 1
From: Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP
Conditional deletion of 4EHP in CaMKIIα-positive excitatory neurons. A Schematic depicting forebrain and hippocampal regions mainly (but not exclusively) targeted for Cre recombinase expression under the promoter of Camk2a. Eif4e2 was flanked with loxP sites on both alleles. B Genotyping confirms WT or homozygous flx/flx mice with Cre recombinase. C Immunofluorescence analysis on the CA1 region of hippocampal coronal slices confirms specific deletion of 4EHP (red) in CaMKII-positive excitatory neurons (green). Hoechst-stained nuclei are in blue, scale bar represents 20 µm. D Western blot analysis further validates loss of 4EHP protein in the hippocampus of P60 mice (WT n = 8, cKOexc n = 9) but not in P0 whole brain (WT n = 8, cKOexc n = 7). E, F are quantifications of D. Data are presented as mean ± s.e.m. **p < 0.01; ns, not significant. P value calculated using an unpaired t-test. Sample size is located within the bar graph for each group