Fig. 5
From: Cell-type-specific translational control of spatial working memory by the cap-binding protein 4EHP
Inhibitory neurons mediate working memory via 4EHP and mTORC1. A Immunofluorescence analysis on prefrontal cortex coronal slices confirms specific deletion of 4EHP (purple) in GAD67-positive inhibitory neurons (green). Hoechst-stained nuclei are in blue, scale bar represents 20 µm. B T-maze memory is shown as a discrimination index for the novel vs. familiar arm where 0% means equal time spent in both arms (WT n = 12, cKOinh n = 13). C Total exploration in either arm. D With weak CFC (0.3 mA, 1 s) training, 4EHP-cKOinh mice (n = 9) show same freezing behavior as WT (n = 9) 24 h after receiving the foot shock. E Immunofluorescence analysis of p-S6 (Ser240/244) in 4EHP WT vs. cKOinh. GAD67-positive inhibitory neurons (identified with white arrows) are displayed in red and p-S6 in green. Scale bar represents 60 µm. F Quantification of Mean Gray Value, which is the Integrated Density normalized to area, revealed no difference in p-S6 fluorescence intensity between 4EHP WT (n = 4) and cKOinh (n = 4). G The number of GAD67 cells were counted in 4EHP-cKOinh (n = 4) vs. WT (n = 4). H Immunofluorescence analysis of p-S6 (Ser240/244) in Rptorflx/flx:Gad2-Cre mouse hippocampus compared to Rptor+/+:Gad2-Cre, scale bar represents 20 µm. I Discrimination index for T-maze working memory in Rptorflx/flx:Gad2-Cre (n = 10) compared to Rptor+/+:Gad2-Cre (n = 10). J Total exploration time of both maze arms. Data are presented as mean ± s.e.m. **p < 0.01; ns, not significant. P value calculated using an unpaired t-test. ###p < 0.001, calculated using one sample t-test. Sample size is located within the bar graph for each group