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Fig. 2 | Molecular Brain

Fig. 2

From: Cancer aggravation due to persistent pain signals with the increased expression of pain-related mediators in sensory neurons of tumor-bearing mice

Fig. 2

Chemogenetic manipulation of sensory nerves promotes tumor growth. A Qualitative observation of hM3Dq-mCherry fluorescence in histological sections. hM3Dq-mCherry (red) was expressed in the lumbar spinal cord [scale bar: 100 μm (A-i)] and lumbar DRG [scale bar: 50 μm (A-ii)]. B Lumbar DRG sections were stained with antibodies specific for CGRP, SP or peripherin (all shown in green). hM3Dq: red, overlay: yellow. Scale bars: 50 μm. C Changes in the pain threshold induced by the activation of sensory neurons of hM3Dq-expressed mice by CNO administration, measured by a plantar test (n = 6, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, ***p < 0.001 vs. Control vector/Ipsi). D Experimental timeline. E Changes in the pain threshold induced by the activation of sensory neurons of hM3Dq-expressed mice by CNO administration under the tumor-bearing state, measured by a plantar test (n = 6, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, ***p < 0.001 vs. Control vector/Ipsi). F Quantitative analysis of tumor volume in the Control + LLC and hM3Dq + LLC groups (n = 10, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, **p < 0.01 vs. Control + LLC). G Bioluminescent images of LLC-luc tumor were used to determine the tumor size at two weeks after the implantation of LLC-luc cells. H Quantitative analysis of tumor volume in the Control + B16 and hM3Dq + B16 groups (n = 24–27, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, **p < 0.01, ***p < 0.001 vs. Control + B16). I Quantitative analysis of tumor volume in the Control + AXT and hM3Dq + AXT groups (n = 10, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, *p < 0.05, **p < 0.01 vs. Control + AXT)

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