Skip to main content
Fig. 4 | Molecular Brain

Fig. 4

From: Cancer aggravation due to persistent pain signals with the increased expression of pain-related mediators in sensory neurons of tumor-bearing mice

Fig. 4

Influence of the suppression of sensory neurons on survival under a cancer pain-state. A Qualitative observation of hM4Di-mCherry fluorescence in histological sections. hM4Di-mCherry (red) was expressed in the lumbar spinal cord [scale bar: 100 μm (A-i)] and lumbar DRG [scale bar: 50 μm (A-ii)]. B Lumbar DRG sections were stained with antibodies specific for peripherin (shown in green). hM4Di: red, overlay: yellow. Scale bars: 50 μm. C Experimental time line and schematic illustration of AXT-luc cell implantation. D Changes in the cancer pain threshold suppressed by the Gi-DREADD system in AXT-luc tumor-bearing mice, measured by a plantar test (n = 9–10, Two-way repeated measures ANOVA with the post-hoc Bonferroni test, *p < 0.05, **p < 0.01, ***p < 0.001, vs. Control vector + AXT-luc/Contra, #p < 0.05, ###p < 0.001, hM4Di + AXT-luc/Ipsi vs. Control vector + AXT-luc/Ipsi). E Kaplan–Meier curves showing survival of AXT-luc tumor-bearing AAV6-hM4Di- or control vector-injected mice (n = 10, Log-rank (Mantel-Cox) test, p = 0.0072)

Back to article page

Molecular Brain

ISSN: 1756-6606