Fig. 5

Contextual and cued fear memory of PDZD8-KO mice. a–j, Percent freezing time (a–e) and distance traveled (f–j) during conditioning (a, f), in a context test performed 1 day (b, g) or 28 days (d, i) after conditioning, and in a cued test with altered context performed 1 day (c, h) or 28 days (e, j) after conditioning in the contextual and cued fear conditioning test. Mice were presented three times with white noise as a conditioned stimulus (CS) for 30 s (horizontal black bars) followed by foot shock as an unconditioned stimulus (US) for the last 2 s of the conditioned stimulus (vertical arrows) during the conditioning session. k Shock sensitivity assessed on the basis of the distance traveled during and after exposure to the unconditioned stimulus. All data are means ± s.e.m. (n = 17 for WT and PDZD8-KO mice). Statistical analysis of differences between genotypes was performed with two-way repeated-measures ANOVA (a–k). For the cued test (c, e, h, j), separate P values are shown for the first and second 3-min periods as well as for the time point of 6 min (vertical arrows). *P < 0.05, **P < 0.01; n.s., not significant. The results of all statistical analysis are provided in Additional file 2: Table S2