Fig. 4

ERK signalling underlies GPER1-induced cLTP and synaptic insertion of GluA1. A Representative confocal images of surface GluA1 labelling in hippocampal neurons (DIV 8–12) in control conditions and after G1, D-AP5, and G1 plus D-AP5. NMDA receptor activation is required for G1 induced trafficking of GluA1-containing AMPA receptors. B Pooled data showing the relative effects on surface GluA1 labelling in control neurons and after exposure to G1, D-AP5 and G1 plus D-AP5. C, D Pooled data illustrating the effects on excitatory synaptic transmission in hippocampal slices. The ability of G1 to induce cLTP was blocked after ERK inhibition with PD98059 (C) but was unaffected in the presence of the PI3K inhibitor, LY294002 (D). E Representative confocal images of surface GluA1 labelling in hippocampal neurons (DIV 7–13) in control conditions, after G1, and in the combined presence of G1 and either PD98059 or U0126. F Pooled data showing the relative effects on surface GluA1 labelling in control neurons and after addition of G1, PD98059, U0126 or in the combined presence of G1 plus either PD98059 or LY294002. The G1-induced increase in GluA1 expression requires ERK signalling