Fig. 4
Gene expression analysis of cancer cells derived from tumor tissue under the activation of CRHPVN neurons. A Experimental schedule for evaluating gene expression in cancer cells derived from tumor tissue under the activation of CRHPVN neurons (A-i). Quantitative analysis of tumor volume in WT/hM3Dq mice and CRH-Cre/hM3Dq mice. All data are presented as the mean ± S.E.M. The data were subjected to a comparative analysis by two-way ANOVA followed by the Bonferroni test: ***p < 0.001 vs. WT/hM3Dq mice (n = 16, A-ii). B Schematic images of the tumor dissociation strategy. The panel on the right is a representative flow cytometric plot. C Quantitative PCR analysis for the expression of various mRNAs in cancer cells derived from tumor tissue. HIF1α (C-i), PGC1α (C-ii), Glut1 (C-iii), VEGFb (C-iv), VEGFa (C-v), COX-2 (C-vi), GR (C-vii), PD-L1 (C-viii), Galectin-9 (C-ix), Arg1 (C-x), TGFb1 (C-xi) and IL-10 (C-xii). Each point represents the mean ± S.E.M. Unpaired t-test: *p < 0.05, **p < 0.01 vs. WT/hM3Dq mice (n = 16)