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Fig. 2 | Molecular Brain

Fig. 2

From: Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device

Fig. 2

Successful formation of CStr circuits in the microfluidic device system. A Axons in cortical neurons (left) and dendrites in striatal neurons (right) successfully extended to each microchannel. Axons in the cortical neuron area and dendrites in the striatal neuron area, identified by immunostaining for the axonal marker Tau and dendritic marker MAP2, respectively. Scale bar: 20 μm. Merged image shows synapse formation between cortical axons and striatal dendrites. Scale bar: 50 μm. B Visualization of all connections in CStr circuits by incubation with Fluo5F-AM. Scale bar: 50 μm. C Representative image of synapse formation in the synapse canal. CStr circuits in the microfluidic device were immunostained for the presynaptic marker synapsin I (green) and postsynaptic marker Shank3 (red). Right, magnified imaged of boxed region. Scale bar: 10 μm

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Molecular Brain

ISSN: 1756-6606