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Fig. 5 | Molecular Brain

Fig. 5

From: Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device

Fig. 5

Measurement of synaptic transmission in CStr circuits in the electrostimulable microfluidic device. A Representative images of vG-pH-mCh fluorescence in response to neural activity (100 AP stimulation) at synapses of CStr circuits. Left to right: resting, ∆F100AP, peak 100 AP, and mCherry. Scale bar: 20 μm. B Kymograph images showing vG-pH-mCh fluorescence at the corresponding ROI of boutons in the synapse canal in response to microfluidic chip stimulation with 100 APs. Arrow indicates point of stimulation. C Representative ensemble traces of vG-pH-mCh fluorescence in response to microfluidic chip stimulation with 50, 100 and 300 APs. D vG-pH-mCh fluorescence in response to microfluidic chip stimulation with 50, 100, and 300 APs, expressed as means ± SEM. [vG-pH-mCh]50AP = 0.42 ± 0.06 a.u. (n = 9); [vG-pH-mCh]100AP = 0.67 ± 0.09 a.u. (n = 10); [vG-pH-mCh]300AP = 1.15 ± 0.16 a.u. (n = 10). E–G Distribution of vG-pH-mCh fluorescence amplitude responses at 50 APs (E; n = 372), 100 APs (F; n = 411), and 300 APs (G; n = 409) in individual boutons. H Representative images of Syn-pH fluorescence in response to neural activity (100 AP stimulation) at synapses of CStr circuits. Left, resting; middle, ∆F100AP; right, peak 100 AP. Scale bar: 20 μm. I Representative ensemble traces of Syn-pH fluorescence in response to microfluidic chip stimulation with 100 APs. J Representative images of Physin-pH fluorescence in response to neural activity (100 AP stimulation) at synapses of CStr circuits. Left, resting; middle, ∆F100AP; right, peak 100 AP. Scale bar: 10 μm. K Representative ensemble traces of Physin-pH fluorescence in response to microfluidic chip stimulation with 100 APs

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