Fig. 6
Monitoring of synaptic retrieval in the microfluidic device. A Top: Representative snapshot images of vG-pH-mCh fluorescence (left) and corresponding mCherry images (right) in the CStr circuit at the end of microfluidic chip stimulation with 100 APs. Bottom: Time-lapse snapshots of the time course of synaptic vesicle endocytosis. Scale bar: 10 μm. B Representative ensemble traces of vG-pH-mCh fluorescence during endocytosis in response to different stimulation conditions (50, 100, and 300 APs). C Endocytosis time constants of vG-pH-mCh fluorescence in response to microfluidic chip stimulation with 50, 100, and 300 APs, expressed as means ± SEM. [τendo]50AP = 14.54 ± 1.08 s (n = 9); [τendo]100AP = 14.24 ± 0.67 s (n = 10); [τendo]300AP = 19.92 ± 1.87 s (n = 10). D–F Distribution of vG-pH-mCh endocytosis time constants at 50 APs (D; n = 381), 100 APs (E; n = 429), and 300 APs (F; n = 425) in individual boutons