Fig. 3

Cerebellar hypoplasia in Ggps1 cKO mice. A Representative HE staining images for mice at various ages. Sagittal cerebellum sections at E18.5, P2, P8 and P16 were used. There was a severe cerebellar foliation defect in Ggps1 cKO mice. The scale bar is 500 μm. B The average area of the cerebellum. There was significant difference between control and Ggps1 cKO mice at P2, P8 or P16 (ns, no significance; **P = 0.009, ***P = 4.3 × 10^–7, ***P = 0.001, respectively; n = 3 mice per group per age). C Representative fluorescence IHC images for NeuN and Calbindin. Sagittal cerebellum sections at P16 were used. The typical 10-foliation pattern was readily recognized in the NeuN + or Calbindin + sections from control mice but not Ggps1 cKO mice at P16. The scale bars were indicated in the images. D The average number of NeuN + cells in the cerebellum. There was significant difference between control and Ggps1 cKO mice at P16 (***P = 9.6 × 10^–7; n = 5 mice per group). E The average number of Calbindin + cells in the cerebellum. There was significant difference between the two groups mice (***P = 0.001; n = 6 mice per group)