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Fig. 1 | Molecular Brain

Fig. 1

From: The deficiency of Maged1 attenuates Parkinson's disease progression in mice

Fig. 1

Expression of Maged1 in MPTP-treated mice and MPP+-intoxicated SH-SY5Y cells. A, B Confocal images showing Maged1 (green) and TH (red) colocalization in the substantia nigra of mice (A) or humans (B);nuclei were counterstained with DAPI (blue).Scale bar: 100 μm. C, D Four intraperitoneal injections of MPTP (20 mg/kg) were administered to male C57BL/6 mice at 2-h intervals daily and total protein was extracted from the midbrain of selected mice (time was calculated from the last injection of MPTP), and then analyzed using western blot to determine the protein level of Maged1.n = 3 mice per group; actin was used as a loading control. E, F SH-SY5Y cells were treated with 200 μM MPP+ for the indicated time, cells were harvested, and protein levels of Maged1 determined using western blot analysis. Experiments were performed in triplicate. Quantitative data are shown as means ± SE. * P < 0.05, ** P < 0.01

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