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Fig. 3 | Molecular Brain

Fig. 3

From: The deficiency of Maged1 attenuates Parkinson's disease progression in mice

Fig. 3

Genetic ablation of Maged1 rescues DA neurons from MPTP toxicity. A Immunofluorescence staining for TH (red) and Iba1 (green) in the substantia nigra of WT or Maged1 KO mice (induced or not induced with MPTP), nuclei were counterstained with DAPI (blue).Scale bar: 200 μm. B, C Quantification of TH-positive cells (B) or Iba1-positive cells (C) in the substantia nigra (% of WT + saline). D Representative images of TH-positive fibers in striatum sections. Scale bar: 200 μm. E Quantitative analysis of the optical density of TH-positive fibers in (D) using ImageJ software. F, G Western blot analysis illustrating the expression of TH in the midbrain; GAPDH was used as a loading control. H, I Western blot analysis illustrating the expression of TH in the striatum; GAPDH was used as a loading control. For A–E, WT + saline: n = 4, KO + saline: n = 4, WT + MPTP: n = 6, KO + MPTP: n = 5. For F–I, n = 4 for each group. Data are shown as means ± SE. **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001

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