Fig. 4

Maged1 deficiency protects dopaminergic neurons against MPP⁺-induced toxicity. A Double-label immunocytochemistry for TH (red) and Tuj1 (green) in primary mesencephalic culture; nuclei were counterstained with DAPI (blue). Scale bar: 200 μm. B High-magnification images of (A). Scale bar: 50 μm. C–E The number of TH-positive dopaminergic neurons (C, n = 6 different fields), mean neurite length (μm) (D, n = 12 TH-positive cells), and neurite branching points in each neuron (E, n = 12 TH-positive cells) in (A, B). F ReNcell VM cells were stably transduced with packaged lentivirus vectors to express tdTomato and differentiated for 12 days followed by NC or si-Maged1 transfection; representative images were acquired after treatment with 200 μM MPP⁺ for 24 h. Scale bar: 100 μm. G Measurements of neurite length from (G, n = 12 tdTomato-labeled cells). Data are shown as means ± SE. * P < 0.05, *** P < 0.001, ^##P < 0.01, ^###P < 0.001. NC normal control, KD knock down