Fig. 5

Analysis of capillary diameter in human tissue. Images in (A) and (B) show immunostainings for laminin in individuals with low (A) and high levels (B) of IAPP. Scale bars: 50 µm (A) and (B), 25 µm (down to the right). Graph in (C) shows quantification of the diameter of laminin enclosed capillaries near laminin enclosed mural cells in individuals with low levels of IAPP (Low IAPP) and high levels of IAPP (High IAPP). Graph in (D) shows quantification of the diameter of laminin enclosed capillaries with no visible mural cells. Images in (E) and (F) show measurement of laminin enclosed mural cells with different heights were image in (E) shows a representative mural cell with low height and (F) represents a mural cell with high height. Scale bar: 5 µm (E) and (F). Black dashed line in (E) and (F) indicate the measured distance. Graph in (G) shows quantification of the height of laminin enclosed mural cells in Low IAPP and High IAPP. Images in (A) and (B) were acquired with the 20 × objective, images in (E) and (F) were acquired with the 40 × objective. Data in (C), (D) and (G) were analyzed using student t-test. Each point represents the diameter of one capillary in (C) and (D). Each point represents one measurement of mural cell height in (G). Significant difference at ***p < 0.001