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Fig. 4 | Molecular Brain

Fig. 4

From: Experience-dependent changes in affective valence of taste in male mice

Fig. 4

Fos FISH assay of single tastant treatment. A Fos FISH assay at the CeA. (Left) Representative images of the CeA after single water or umami treatment. Fos-positive cell counts/1 mm2 were not significantly different. Water, n = 32 slices from N = 8 mice; umami, n = 28 from N = 7. B, C Double Fos FISH assay with Sst or Prkcd markers. The ratios of Fos-positive neurons per each marker were not significant. Open and filled triangles indicate single- and double-positive cells, respectively. Sst: water, n = 16 from N = 4; umami, n = 16 from N = 4; Prkcd: water, n = 20 from N = 5; umami, n = 28 from N = 7. D, G–I Fos FISH assay in the PB, NTS, VTA, and IC, which are upstream regions of the CeA. Fos-positive cell counts/1 mm2 were not significantly different. PB: water, n = 24 from N = 6; umami, n = 24 from N = 6; NTS: water, n = 20 from N = 5; umami, n = 24 from N = 6; VTA: water, n = 16 from N = 4; umami, n = 16 from N = 4; IC: water, n = 16 from N = 4; umami, n = 16 from N = 4. E, F Double Fos FISH assay with Calca or Adcyap1 markers in the PB. The ratios of Fos-positive neurons per each marker were not significant. Filled triangles indicate double-positive cells. Calca: water, n = 24 from N = 6; umami, n = 24 from N = 6; Adcyap1: water, n = 24 from N = 6; umami, n = 24 from N = 6. Each scale bar represents 25 μm. *p < 0.05, ****p < 0.0001 (unpaired t-test)

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