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Fig. 5 | Molecular Brain

Fig. 5

From: Experience-dependent changes in affective valence of taste in male mice

Fig. 5

Fos FISH assay of prolonged taste exposure mice. A Fos FISH assay at the CeA. (Left) Representative images of the CeA of the prolonged taste exposure mice after water or umami stimulation. Fos-positive cell counts/1 mm2 were increased by umami stimulation in the umami-exposed mice. Water, n = 24 slices from N = 6 mice; umami, n = 24 from N = 6. B, C Double Fos FISH assay with Sst or Prkcd markers. The ratio of Fos-positive neurons per each marker was increased in the Prkcd-positive neurons. Sst: water, n = 20 from N = 5; umami, n = 24 from N = 6; Prkcd: water, n = 16 from N = 4; umami, n = 16 from N = 4. D, G–I Fos FISH assay in the PB, NTS, VTA, and IC, which are upstream regions of the CeA. Fos-positive cell counts/1 mm2 were not significantly different. PB: water, n = 24 from N = 6; umami, n = 24 from N = 6; NTS: water, n = 12 from N = 3; umami, n = 12 from N = 3; VTA: water, n = 20 from N = 5; umami, n = 24 from N = 6; IC: water, n = 16 from N = 4; umami, n = 16 from N = 4. E, F Double Fos FISH assay with Calca or Adcyap1 markers in the PB. The ratios of Fos-positive neurons per each marker were not significant. Calca: water, n = 24 from N = 6; umami, n = 24 from N = 6; Adcyap1: water, n = 20 from N = 5; umami, n = 20 from N = 5. Each scale bar represents 25 μm. Arrows indicate Fos-positive cells. Open and filled triangles indicate single- and double-positive cells, respectively. *p < 0.05, ***p < 0.001 (unpaired t-test)

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