Fig. 3

Evaluation for the knockdown efficiency of Scop shRNA expressing lentiviral vector. a Schematic diagram of the shRNA-expressing lentiviral vector. The shRNA is controlled by the macaque H1 promoter (mkH1), and the CMV promoter drives the EGFP marker gene for tracking transduced cells. 5′-LTR, HIV-1 5′-LTR; 3′ LTR, HIV-1 self-inactivating 3′-LTR. b Western blot analysis of SCOP protein level after shRNA expressing lentivirus infection. Decrease in SCOP protein level in COS7 cells by infection of anti-Scop shRNA lentivirus (shScop). Scramble shRNA lentivirus was used as a control. The sample in each lane is from a different culture dish. The displayed blot was cropped from the full-length blot in the Additional file 1