Fig. 2
From: FMRP binds Per1 mRNA and downregulates its protein expression in mice
FMRP interacts with core circadian transcript Per1 mRNA. A U2OS cells expressing Flag-FMRP were subjected to RIP assay. Immunoblot confirmed the pull-downed Flag-tagged FMRP. B RT–qPCR analysis shows binding of FMRP to Per1 mRNA in U2OS cells. Data presented as means ± SEM (n = 3). ****P < 0.0001; two-tailed Student’s t-test. C Agarose gel electrophoresis of RT-PCR reactions from RIP assay