Fig. 4

SOX2OT knockdown prevents LPS-induced mitochondrial fission. a Mitochondrial morphology in the hippocampus determined using transmission electron microscopy. Scale bar = 0.5 μm. Analysis of mitochondrial damage score. P < 0.005 vs Sham. b Mitochondrial morphology in HT22 cells obtained using MitoTracker Red staining. Original magnification × 600; n = 4. c Percentage of cells with fragmented mitochondria. d: Mean mitochondrial volume (fold over Con + Nc); n = 4. e Expression of the Drp1, Fis1, Mfn, and Opa1 proteins determined using western blotting. *P < 0.05, ****P < 0.0001 vs. Lv-neg; ^##P < 0.01 vs. Lv-neg + LPS; ^&&P < 0.009 vs. Lv-SOX2OT groups; n = 4