Fig. 6

SOX2OT knockdown attenuated surgery-induced cognitive dysfunction and promoted downstream SOX2 expression in elderly mice. a Experimental design and workflow. b The lentivirus Sox2ot was injected bilaterally into the CA1 region of the mouse hippocampus. c The GFP signal observed in CA1 neurons 7 days after injection of lentivirus confirmed the successful expression of Sox2ot or the control vector. Scale, 100 μm. d SOX2OT mRNA measured using RT-qPCR. **P < 0.001 vs Lv-neg. e Comparison of total activity, distance, and time spent in the central square; n = 4. f Comparison of total sniffing time and preference index for novel object. **P < 0.001 vs. Lv-neg group; n = 4. g Freezing time. **P < 0.005 vs. Lv-neg. e SOX2OT mRNA measured using RT-qPCR. **P < 0.001 vs Lv-neg. h SOX2 protein expression analyzed using western blotting. ***P < 0.0001, ^#P < 0.03, ^&&&P < 0.0001 vs Lv-neg. i Drp1 protein expression analyzed using western blotting. *P < 0.04, ^###P < 0.0006, ^&&P < 0.003 vs Lv-neg; n = 4