Fig. 7

SOX2OT binds to the downstream SOX2 target, which is related to mitochondrial fission protein Drp1. a Evaluating the transfection efficiency of Lv-SOX2OT lentivirus by detecting the expression of SOX2 using western blotting. *P < 0.05 vs. Lv-neg group, ^#P < 0.05 vs. Lv-SOX2OT group, ^&& P < 0.004 vs. Lv-neg + LPS group; n = 4. b RIP-qPCR to verify the binding of downstream SOX2 target with lncRNA SOX2OT. *P < 0.05 vs. IgG group; n = 3. c Verifying the transfection effect of siRNA by detecting the expression of SOX2 using western blotting. **P < 0.007 vs. si-nc group, ^#### P < 0.0001 vs. si-SOX2 group; ^&&P < 0.004 vs. si-nc + LPS group; n = 4. d Flow cytometry analysis and JC-1 quantitative determination of mitochondrial membrane potential in HT22 cells. ****P < 0.0001 vs. si-nc group; ^###P < 0.001 vs. si-SOX2 group; ^&&P < 0.004 vs. si-nc + LPS group; n = 4. e Drp1 expression detected using western blotting after transfection with si-NC or si-SOX2 vectors. **P < 0.006 vs. si-nc group; ^###P < 0.0001 vs. si-SOX2 group; ^&&P < 0.009 vs. si-nc + LPS group; n = 4. f The drp1 mRNA level measured using RT-qPCR.^***P<0.001 vs. si-NC; ^&&&P<0.001 vs. si-Nc+LPS; ^###P<0.001 vs. si-SOX2. g Analysis of the binding reactivity between SOX2 and drp1 promoter region using Luciferase reporter assay. ***P < 0.0006 vs.pGL3-drp1 ^#P < 0.05 vs. pGL3-drp1-WT + SOX2; n = 3