Fig. 1

Behavioral changes following acute social isolation in Shank2^−/− mice. A Experimental scheme of three-chamber test following group-housing and social isolation. B Heat maps of WT grouped, WT isolated, KO grouped and KO isolated male mice. C Isolation increases the sociability level both in WT and KO mice (Sniffing time; WT grouped, p**** < 0.0001; WT isolated, p**** < 0.0001; KO grouped, p > 0.9999; KO isolated, p = 0.0918; time spent with stranger; WT grouped vs. WT isolated, p^# = 0.0519; KO grouped vs. KO isolated, p*** = 0.0007; WT grouped vs. KO grouped, p** = 0.0094). D Total distance moved and velocity are increased in KO isolated male mice (Total distance moved; WT grouped vs. WT isolated, p = 0.1487; KO grouped vs. KO isolated, p**** < 0.0001; WT grouped vs. KO grouped, p = 0.5388; velocity; WT grouped vs. WT isolated, p = 0.1371; KO grouped vs. KO isolated, p**** < 0.0001; WT grouped vs. KO grouped, p = 0.5224). E Heat maps of four types of groups in female mice. F The sociability level is increased in KO mice following social isolation (Sniffing time; WT grouped, p**** < 0.0001; WT isolated, p*** = 0.0001; KO grouped, p > 0.9999; KO isolated, p* = 0.0381; time spent with stranger; WT grouped vs. WT isolated, p > 0.9999; KO grouped vs. KO isolated, p** = 0.0080; WT grouped vs. KO grouped, p** = 0.0023). G The mobility level is not affected by social isolation (Total distance moved; WT grouped vs. WT isolated, p = 0.1822; KO grouped vs. KO isolated, p > 0.9999; WT grouped vs. KO grouped, p** = 0.0025; velocity; WT grouped vs. WT isolated, p = 0.1838; KO grouped vs. KO isolated, p > 0.9999; WT grouped vs. KO grouped, p** = 0.0024). Additional statistical information is in Additional file 2