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Fig. 4 | Molecular Brain

Fig. 4

From: Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells

Fig. 4

Inhibition of mitochondrial fission suppresses primary ciliogenesis in HSPA9-depleted SH-SY5Y cells. (A, B) SH-SY5Y cells were transfected with HSPA9 (siHSPA9) with or without Drp1 siRNA (siDrp1) for 3 days or treated with Mdivi-1 (10 µM) for 24 h. Mitochondrial fragmentation was confirmed using a Mito-tracker probe (red), and the cells were analyzed via western blotting with the indicated antibodies (A) (Scale bar, 20 μm). The mitochondrial length was further measured (B). (C, D) SH-SY5Y cells transfected with HSPA9 siRNA (siHSPA9) were transfected with Drp1 siRNA (siDrp1) or treated with Mdivi-1 (10 µM). The cells were then immunostained with ARL13B (green) and Hoechst 33,342 dye (blue) (C) (Scale bar, 5 μm). Ciliated cells and the cilium length were measured in approximately 200 cells per group (D). The experiments were repeated at least three times. Data are presented as the mean ± SEM. (n = 3, ** p < 0.01, **** p < 0.001)

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Molecular Brain

ISSN: 1756-6606