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Fig. 5 | Molecular Brain

Fig. 5

From: Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells

Fig. 5

Primary cilia prevent neurotoxicity by activating AKT in HSPA9-depleted SH-SY5Y cells. (A, B) SH-SY5Y cells co-transfected with siHSPA9 with or without siIFT88 were analyzed via western blotting with the indicated antibodies (A). Cell viability was determined through the CCK-8 assay. (C, D) SH-SY5Y cells transiently transfected with scrambled siRNA (Sc) or siHSPA9 were treated with an AKT inhibitor, MK-2206 (5 µM), for 24 h. The cells were then harvested to analyze protein expression via western blotting (C) and to determine cell viability (D). Data were obtained from at least three independent experiments and the results are presented as the means ± S.E.M. (n = 3, ** p < 0.01, *** p < 0.005)

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