Fig. 1

Ablation of GluK1 in GABAergic interneurons attenuates GABAergic synaptic activity in the neonatal but not juvenile or adult CA3. A Example traces of whole-cell patch-clamp recordings from neonatal (i), juvenile (ii) and adult (iii) control (left) and Gad-Grik1^−/− (right) mice with sIPSCs appearing as outward and sEPSCs as inward currents. B, C Basal frequency of sIPSCs (B) and sEPSCs (C) in pyramidal CA3 cells from acute control and Gad-Grik1^−/− mice slices across different age groups (neonatal: n = 30 (21) and 24 (19); juvenile: n = 14 (12) and 13(11); adult: n = 11 (10) and 9 (9), for control and Gad-Grik1^−/− respectively; n refers to number of cells, followed by number of animals in parenthesis. Bars represent mean ± SEM. Frequencies were compared by 2-way ANOVA with multiple Mann–Whitney test as a post-hock to detect differences in genotype for each age group. **** p < 0.000001 (Mann–Whitney test)