Fig. 2

Pharmacological characterization of spontaneous synaptic activity in Gad-Grik1^−/− mice. A Example traces of recordings from neonatal (i and iv), juvenile (ii) and adult (iii) control (left columns) and Gad-Grik1^−/− (right columns) slices, before (baseline) and during ATPA (i, ii, iii) or ACET (iv) application. B Pooled data illustrating the effect of ATPA (1 µM) on sIPSC frequency in CA3 pyramidal cells from acute control and Gad-Grik1^−/− slices at different stages of development (neonatal: n = 14 (10) and 10 (10); juvenile: n = 10 (10) and 8 (7); adult: n = 5 (5) and 6 (6), for control and Gad-Grik1^−/−, respectively). C Effect of ATPA on sEPSC frequency, for the same cells as in B. D Effect of ACET (200 nM) on the frequency of sEPSCs and sIPSCs in neonatal control and Gad-Grik1^−/− slices (n = 13 (N = 10) and 7 (6), for control and Gad-Grik1^−/−, respectively). Bars represent mean ± SEM. Frequency of events is normalized to the baseline (dashed line). Activity during ATPA / ACET application is compared to the baseline by paired t-test or Wilcoxon paired test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05