Alpha-CaMKII deficiency causes immature dentate gyrus, a novel candidate endophenotype of psychiatric disorders

Elucidating the neural and genetic factors underlying psychiatric illness is hampered by current methods of clinical diagnosis. The identification and investigation of clinical endophenotypes may be one solution, but represents a considerable challenge in human subjects. Here we report that mice heterozygous for a null mutation of the alpha-isoform of calcium/calmodulin-dependent protein kinase II (alpha-CaMKII+/-) have profoundly dysregulated behaviours and impaired neuronal development in the dentate gyrus (DG). The behavioral abnormalities include a severe working memory deficit and an exaggerated infradian rhythm, which are similar to symptoms seen in schizophrenia, bipolar mood disorder and other psychiatric disorders. Transcriptome analysis of the hippocampus of these mutants revealed that the expression levels of more than 2000 genes were significantly changed. Strikingly, among the 20 most downregulated genes, 5 had highly selective expression in the DG. Whereas BrdU incorporated cells in the mutant mouse DG was increased by more than 50 percent, the number of mature neurons in the DG was dramatically decreased. Morphological and physiological features of the DG neurons in the mutants were strikingly similar to those of immature DG neurons in normal rodents. Moreover, c-Fos expression in the DG after electric footshock was almost completely and selectively abolished in the mutants. Statistical clustering of human post-mortem brains using 10 genes differentially-expressed in the mutant mice were used to classify individuals into two clusters, one of which contained 16 of 18 schizophrenic patients. Nearly half of the differentially-expressed probes in the schizophrenia-enriched cluster encoded genes that are involved in neurogenesis or in neuronal migration/maturation, including calbindin, a marker for mature DG neurons. Based on these results, we propose that an "immature DG" in adulthood might induce alterations in behavior and serve as a promising candidate endophenotype of schizophrenia and other human psychiatric disorders.

were significantly changed. Strikingly, among the 20 most downregulated genes, 5 had highly selective expression in the DG. Whereas BrdU incorporated cells in the mutant mouse DG was increased by more than 50 percent, the number of mature neurons in the DG was dramatically decreased. Morphological and physiological features of the DG neurons in the mutants were strikingly similar to those of immature DG neurons in normal rodents. Moreover, c-Fos expression in the DG after electric footshock was almost completely and selectively abolished in the mutants. Statistical clustering of human post-mortem brains using 10 genes differentially-expressed in the mutant mice were used to classify individuals into two clusters, one of which contained 16 of 18 schizophrenic patients. Nearly half of the differentially-expressed probes in the schizophreniaenriched cluster encoded genes that are involved in neurogenesis or in neuronal migration/ maturation, including calbindin, a marker for mature DG neurons. Based on these results, we propose that an "immature DG" in adulthood might induce alterations in behavior and serve as a promising candidate endophenotype of schizophrenia and other human psychiatric disorders.

Background
Elucidating the neural and genetic factors underlying psychiatric illness is hampered by the current methods of clinical diagnosis [1]. The identification and investigation of clinical endophenotypes might be one solution [2], but represents a considerable challenge in human subjects. Therefore, establishing animal models of psychiatric disorders is essential for understanding the pathogenesis/ pathophysiology of the disorders [3][4][5][6]. Previously, we reported that forebrain-specific calcineurin (CN) knockout mice have severe working/episodic-like memory deficits [7], and exhibit multiple abnormal behaviors related to schizophrenia [8]. Schizophrenia is significantly associated with a variation in the 8p21.3 gene, PPP3CC, which encodes the CNA gamma subunit of calcineurin [9][10][11]. Based on these findings, we speculated that we could efficiently obtain a mouse model of psychiatric disorders by applying a comprehensive behavioral test battery [12] to various strains of mice bearing mutations of the genes encoding the molecules involved in CN signaling pathways or CN related neural mechanisms [13]. We assessed seven different strains of mutant mice: mice lacking type 3 isoform ryanodine receptor, neuronal nitric oxide synthase, adenomatous polyposis coli, calcium/calmodulindependent protein kinase IV, pituitary adenylate cyclaseactivated polypeptide, nuclear factor of activated T cells c2/c3/c4 [14] or alpha-isoform of calcium/calmodulindependent protein kinase II (alpha-CaMKII). Four strains exhibited increased locomotor activity, and three strains exhibited abnormal social behavior (Miyakawa, unpublished observations). Among them, the only mutant mouse strain that exhibited a significant working memory deficit, a proposed functional endophenotype of schizophrenia and other psychiatric disorders [15], was heterozygous for a null mutation of the alpha-isoform of CaMKII (alpha-CaMKII+/-) ( Figure 1A and 1B). CaMKII is a ubiquitous serine/threonine protein kinase that is abundant in the brain (up to 2% of the total protein); a holoenzyme that consists of four isozymes (α, β, γ, δ); phosphorylates protein substrates, such as AMPA recep-tors, synapsin I, tyrosine hydroxylase, L-type Ca 2+ channels, and MAP-2, and itself by autophosphorylation; and is important for long-term potentiation, synaptic plasticity, and memory formation [16][17][18]. CaMKII is situated downstream of CN in a model [19].
Here we report that alpha-CaMKII+/-mice have profoundly dysregulated behaviors and impaired neuronal development in the DG. The behavioral abnormalities include a severe working memory deficit and an exaggerated infradian rhythm, which are similar to symptoms seen in schizophrenia and other psychiatric disorders. Transcriptome analysis of the hippocampus of these mutants revealed that the expression levels of more than 2000 genes were significantly changed. Strikingly, among the 20 most downregulated genes, 5 had highly selective expression in the DG. Whereas BrdU incorporated cells in the mutant mouse DG was increased by more than 50 percent, the number of mature neurons in the DG was dramatically decreased. Morphological and physiological features of the DG neurons in the mutants were strikingly similar to those of immature DG neurons in normal rodents. Statistical clustering of human post-mortem brains using 10 genes differentially-expressed in the mutant mice were used to classify individuals into two clusters, one of which contained 16 of 18 schizophrenic patients. Nearly half of the differentially-expressed probes in the schizophrenia-enriched cluster encoded genes that are involved in neurogenesis or in neuronal migration/ maturation. Based on these results, we propose that an "immature DG" in adulthood might induce alterations in behavior and serve as a promising candidate endophenotype of human psychiatric disorders.

Severe working memory deficits and exaggerated infradian rhythm in alpha-CaMKII+/-mice
Alpha-CaMKII+/-mice have decreased anxiety-like behavior, increased aggressive behavior [20], and deficits in long-term memory and the establishment of permanent In the spatial working memory version of the eight-arm radial maze, the alpha-CaMKII+/-mice performed significantly worse than control mice with respect to the number of different arm choices in the first eight entries (P = 0.0022) and made significantly more revisiting errors than controls (P = 0.0026). (C) Mutant mice had normal performance in reference memory tasks in the eight-arm radial maze (P = 0.6394). Activity level of control mice (D-F) and alpha-CaMKII+/-mice (G-I) in their home cage. Mutant mice were hyperactive and showed a periodic mood-change-like activity pattern. (J) The variability in the activity in the home cage during the dark period was greater in mutant mice (coefficient of variation, P = 0.0088, Mann-Whitney U test). (K) The activity of mutant mice increased throughout the dark period (genotype effect, P < 0.0001, interaction between genotype and time, P < 0.0001, repeated measures ANOVA). Error bars indicate s.e.m. memories [21,22]. Consistent with a previous report [20], we found that alpha-CaMKII+/-mice also exhibit high levels of aggression towards cage mates: By the age of 3 mo, more than half of their cage mates, both wild-type controls and mutants, were killed by the mutants. While severe working memory deficits were observed in both in the eight-arm radial maze task ( Figure 1A and 1B) and delayed alternation task using a modified T-maze (data not shown), their performance in reference memory tasks using the same apparatuses were normal ( Figure 1C). In addition to their severe cognitive deficits, these mutants showed an array of other profoundly dysregulated behaviors. The behavioral phenotypes of these mice include increased locomotor activity in novel situations, decreased anxiety-like behavior, and decreased depression-like behavior (see Additional file 1, Figure S1). Normal mice have a constant activity pattern in their home cage ( Figure 1D-1F). The pattern of locomotor activity of the mutants, however, changed over time ( Figure 1G-1I). They exhibited periodic mood-change-like behavior in their home cage. One cycle was approximately 1 to 2 wk. The mutants had more variable activity patterns in the total distance traveled in the dark period in their home cages ( Figure 1J). The locomotor activity pattern of the mutants during a single day was also remarkably different from that of control mice. While wild-type mice generally had two peaks in activity during the dark phase, the activity of the mutant mice steadily increased until morning ( Figure 1K).

Selective changes in dentate gyrus of alpha-CaMKII+/mice
These marked changes in behavior led us to examine the brains of the alpha-CaMKII mutants. In the mutants, the amount of alpha CaMKII was decreased by 20% to 60% in the cingulate cortex, amygdala, and hippocampus, and the amount of phosphorylated beta-CaMKII was increased in the hippocampus, which is probably a compensatory effect. The amounts of proteins, such as CN and phosphorylated extracellular signal-regulated kinase, were also altered (see Additional file 1, Figure S2). There were no dramatic differences in monoamine content in any region investigated, except for an increase in striatal dopamine turnover in the mutant mice (see Additional file 1, Figure S3). Next, we analyzed the transcriptome of the hippocampus, a region that has an essential role in working memory and locomotor activity in rodents, of alpha-CaMKII+/-mice, by using Affymetrix GeneChips. There were more than 2000 genes that were significantly up-or downregulated (see Additional file 2, Table S1 and S2). There was a more than two-fold increase in the gene expression levels of dopamine D1A receptors, therefore we performed autoradiography studies for five major neurotransmitter receptors and two transporters (see Additional file 1, Figure S4 and S5). Consistent with the microarray results, dopamine D1-like receptor binding was dramatically and selectively increased in the DG of alpha-CaMKII+/-mice (Figure 2A and 2B). N-methyl-Daspartate (NMDA) receptor binding was downregulated in the hippocampus, especially in the DG ( Figure 2C and 2D). Based on the highly selective changes in receptor binding, we examined the location of genes whose expression levels were changed in the mutant brain. A database search using Allen Brain Atlas [23]http://www.brainmap.org/ revealed that, among the 20 most downregulated genes in the alpha-CaMKII +/-hippocampus, 5 genes (DSP, TDO2, NPNT, IL1R1, and PNCK) had highly selective expression in the DG ( Figure 2E-2J, and Additional file 1, Figure S6). Moreover, c-Fos expression in the DG 2 h after electric shock was almost completely and selectively abolished in alpha-CaMKII+/-mice, which suggests that their DG was functionally downregulated (Figure 2K, 2L and Additional file 1, Figure S7). These findings in the DG, a region in which 3000 to 4000 neurons are born each day in rodents [24], led us to examine the cell proliferation in the mutant mouse DG. BrdU-labeled cells were dramatically increased by 53% in the mutants (Figure 3A). Consistent with this finding, many of the genes involved in the brain derived neurotrophic factormitogen-activated protein kinase (BDNF-MAPK) pathway, which has an important role in neurogenesis [25], were significantly upregulated or downregulated in the hippocampus of the alpha-CaMKII+/-mice, including a 32 to 35% up-regulation of BDNF probes (see Additional file 1, Figure S8). Dysregulation of the expression of BDNF-MAPK pathway genes could be related to the increased cell proliferation in the mutants.

The dentate gyrus of alpha-CaMKII+/-mice is immature
There are distinct stages in adult neurogenesis in the DG. Each stage has a few markers and specific morphological and physiological properties [24,26]. The number of cells expressing Polysialic acid-NCAM (PSA-NCAM), a marker for late-stage progenitor cells and immature neurons, and calretinin, a marker for immature neurons, was dramatically increased in the DG of the mutant mice. In normal mice, after granule cells migrate into the granule cell layer, PSA-NCAM positive cells were hardly detected in the granule cell layer. In the mutants, however, there were many PSA-NCAM positive cells in the granule cell layer. Moreover, the amount of calbindin, a marker for mature neurons in the DG, -positive cells was dramatically reduced ( Figure 3B and 3C). Thus, in the mutants, the number of immature neurons is increased and the number of mature neurons is decreased (in this report, "immature" means that the control of the precursor cell proliferation and the functional differentiation of the newly born cells were hampered.). Furthermore, rapid Golgi staining in the mutant DG showed that dendritic branching and length were decreased, which are the morphological properties Specific Abnormalities in the DG of Alpha-CaMKII+/-Mice Available from: http://www.brain-map.org.). Graphs indicate the relative expression levels of each gene in the microarray experiment, which are confirmed by quantitative RT-PCR (see Additional file 1, Figure S6). (K, L) c-Fos expression following electric foot shock was selectively decreased in the DG of mutant mice at the age of 8 weeks. BLA, basolateral amygdaloid nucleus; MeA, medial amygdaloid nucleus; LimCX, limbic cortex; Ent, entorhinal cortex; CPU, caudate nucleus/putamen; NAC, nucleus accumbens; SNr, substantia nigra pars reticulata; CA1rad, stratum radiatum of CA1; CA1or, stratum oriens of CA1; MDT, mediodorsal thalamic nucleus; CTX, cingulated cortex; SEP, septum; OrCX, orbital cortex; TGN, tegmental nuclei; CB, cerebellum; Ncx, neocortex; LH, lateral hypothalamus; CeA, central amygdaloid nucleus. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Error bars indicate s.e.m. of immature neurons ( Figure 3D and Additional file 1, Figure S9). Consistent with these immunohistochemical and morphological findings, the neurons in mutant DG had electrophysiological features that are characteristic of immature DG neurons [27][28][29], including high input resistance, high excitability, small spike amplitude and a decreased number of spikes during sustained depolarization ( Figure 4A-4D, and Additional file 1, Figure S10). Moreover, the mutants had extremely abnormal transmission at the synapses between granule cell axons, mossy fibers (MFs), and the CA3 pyramidal cells. That is, basal transmission was increased and large facilitation characteristic of the MF synapse was markedly decreased in mutants ( Figure 4E-4G, and Additional file 1, Figure  S10). The synaptic facilitation is generally known to be mediated by presynaptic mechanisms. Since the frequency facilitation at the MF synapse is very small at the early postnatal developmental stages in normal mice [30], the greatly reduced MF facilitation in the mutant mice lends further support to the hypothesis that the mutant granule cells in alpha-CaMKII+/-mice are immature. At the ultra structural level, the MF-CA3 synapses were poorly developed in the hippocampus of the mutant mice (see Additional file 1, Figure S11), which could be related to the abnormal transmission at the synapse. Collectively, results of analyses using molecular markers, morphological assessment using Golgi staining, and electrophysiological analysis using whole cell patch clamp all indicated that the vast majority of the mutant DG granule cells failed to develop into mature neurons. The qualitative difference in the DG granule cell developmental stage accompanied by the severely impaired neuronal functions might also account for the high number of genes that were differentially expressed in the mutant hippocampus.

Human post-mortem brain analysis using the biomarkers derived from alpha-CaMKII+/-mice
To assess if our findings derived from the mutant mice could be related to human psychiatric disorders, comprehensive gene expression data of human post-mortem brains was analyzed using a specific biomarker set derived from alpha-CaMKII+/-mice. In this study, we selected 10 genes (ADCY8, CCND1, LOC151835, LOC284018, NTNG1, PDYN, PIP3-E, PNCK, SPATA13, and TDO2), which were differentially expressed in the mutant hippocampus, as a set of biomarkers to characterize the mutants (see Additional file 1, Figure S12). Statistical clustering of the expression data of these 10 genes in 166 hippocampi of human post-mortem brains was performed. The analysis classified the subjects into two clusters; one cluster contained 16 of 18 schizophrenic patients and 1 schizoaffective and 2 bipolar patients ( Figure 5A). Further, we compared the gene expression profile between the schizophrenic patients in the schizophrenia-enriched cluster and the controls with no major psychiatric diagnosis in the control-enriched cluster, and found 26 differentiallyexpressed probes in the schizophrenic patients. Almost half of these differentially-expressed genes are involved in neurogenesis or neuronal migration/maturation, including calbindin, a maker for mature neurons in DG ( Figure  5B, Table 1, Additional file 1, Figure S12 and S13, and Additional file 2, Table S3 and S4). These results indicate that schizophrenia subgroup could be classified using the biomarkers derived from the alpha-CaMKII+/-mice, and that the functional alterations in the hippocampus could be a potential intermediate phenotype for this schizophrenia subgroup, consistent with proposals that the hippocampus is central to the pathophysiology of schizophrenia [31,32].

Discussion
In this study, alpha-CaMKII+/-mice had dysregulated behaviors, such as increased locomotor activity, a severe working memory deficit, and exaggerated infradian rhythm. Transcriptome analysis and comprehensive autoradiography studies indicated that the mutants had marked abnormalities in gene expression and receptor binding in the hippocampus, specifically in the DG. Results of analyses using molecular markers, morphological assessment using Golgi staining, and electrophysiological analysis using the whole cell patch clamp technique were consistent with the idea that most of the neurons in the DG of the mutant mice failed to develop to maturity, which likely caused the functional impairment of the granule cells. To our knowledge, this is the first in vivo study showing that an immature portion of a brain can be embedded in an adult brain of animals. Furthermore, our transcriptome analysis of human post-mortem brain revealed that biomarkers derived from mutant mice could be used to classify humans into two clusters, one of which had a higher susceptibility to schizophrenia and differentially expressed genes related to neural development, including calbindin, a marker for mature DG neurons. These findings suggest that the "immature DG" or its equivalent is potentially common internal brain phenotype reflecting or affecting behavioral traits and susceptibility to psychiatric disorders.
These findings raise the question of how alpha-CaMKII deficiency causes immature DG phenotype. CaMKII is implicated in dendrite morphogenesis and stabilization [33][34][35][36]. Based on the fact that the DG phenotype of the mutants is accompanied by changes in the expression of many genes, it is reasonable to speculate that alpha-CaM-KII contributes to regulate the gene expression required for the maturation of DG neurons. The CaMKII-NeuroD pathway specifies dendritic morphogenesis in primary granule neurons in cerebellar slices [34]; CaMKII phosphorylates a transcription factor NeuroD at Ser336 in an activity-dependent manner and thereby orchestrates a Immature DG of Alpha-CaMKII+/-Mice Figure 3 Immature DG of Alpha-CaMKII+/-Mice. (A) The number of BrdU-positive cells in the subgranular zone of the DG is increased in the alpha-CaMKII +/-mice at 7 weeks old, as observed 1 d after BrdU administration. The number of BrdU-positive cells within the DG was 52.8% higher (p = 0.00001) in the alpha-CaMKII +/-mice than in the wild-type mice. (B) In alpha-CaMKII+/-mice, the number of cells expressing calbindin (a mature-neuron marker) was decreased. (C) In alpha-CaMKII+/mice, the number of cells expressing PSA-NCAM (a late-progenitor and immature-neuron marker) and calretinin (an immature-neuron marker) was markedly increased. CaMKII was co-expressed in a subset of PSA-NCAM-, calretinin-, and calbindinexpressing cells, indicated by the white arrowheads. For the analysis of expression of markers related to neurogenesis and differentiation, at least five mice were examined in each group. (D) Rapid Golgi staining showed that the number of Golgi-impregnated cells was decreased in the DG of alpha-CaMKII+/-mice (see Additional file 1, Figure S9) and that Golgi-impregnated cells in the DG of alpha-CaMKII+/-mice had less branching and shorter dendrites. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Error bars indicate s.e.m.
program of gene expression and stimulates dendritic growth. Although the brain region investigated by Gaudilliere et al. was not the DG, considering the high expression of NeuroD in the hippocampus [37], it is possible that the impaired CaMKII-NeuroD pathway underlies the abnormality of DG maturation of the mutants. Also, Greenberg and colleagues reported that activity-dependent phosphorylation of MeCP2 at serine 421 is mediated by CaMKII and phosphorylation of the site controls the ability of MeCP2 to regulate activity-dependent BDNF transcription and dendritic growth [35], raising the possibility that the immature DG of the mutant mice is due to a disruption of the program that regulates gene expression triggered by this pathway. The changes in expression of many genes involved in the BDNF-MAPK pathway in the hippocampus of the mutant mice (see Additional file 1, Figure S8) is of interest in relation to their findings. Degradation of scaffold protein Liprina1 by alpha-CaMKII regulates leukocyte common antigen-related receptor tyrosine phosphatase distribution and dendrite development [36]. Impairment of this mechanism could provide an alternative or additional candidate for the development of an immature DG in the mutants. Further studies are required to specify the mechanisms responsible for the immaturity of the DG neurons of the mutants as well as the factors making the DG particularly sensitive to the amount of CaMKII compared to other brain areas.
The DG neurons in the mutants also had remarkable functional abnormalities, including higher input resistance, slightly depolarized resting membrane potentials, high excitability, decreased number of spikes during sustained depolarization, and greatly reduced facilitation at MF-CA3 synapses. The morphological immaturity, that is, the reduced dendritic arborization, is consistent with the higher input resistance of the mutant DG neurons [29]. Although the other electrophysiological properties are hard to explain based solely on the morphological immaturity, they are very similar to those of immature DG neurons of normal adult animals [27] or neurons in the early postnatal period [30]. Those unique physiological properties might be explained by some of the differentially expressed channels/receptors and their functional modulators found in our gene chip analysis and binding assays but, given the changes in expression of more than 2000 genes, identifying the actual determinants of those properties requires understanding the neuron as a complex system regulated by a network of a number of molecules, and not just a single pathway.
The selective and all-or-none type reduction of c-Fos expression in the DG after behavioral stimulation is likely to reflect the lower ability of the neurons to keep firing after stimulation in the mutant mice. It is easy to imagine that such extreme functional abnormalities of firing properties of neurons, together with marked impairment of synaptic facilitation at MF-CA3 synapses, change the DG function in a manner qualitatively different from normal DG, which would subsequently cause abnormal DGdependent behavior. Because the alpha-CaMKII+/-mice we used are conventional global knockout mice, it is technically difficult to determine the specific brain region that caused each behavioral abnormality. The dramatic and specific abnormalities in the DG, as assessed using several different assays, however, strongly suggest that at least some of the behavioral deficits are due to the "immature DG". Indeed, the DG has an important role in locomotor activity [38] and the formation of spatial working memory [38,39], and adult neurogenesis in the hippocampus is related to anxiety-related behavior [40], voluntary exercise [41], and memory formation [42]. Also, it is possible that DG dysfunction during development could cause secondary functional alterations in other areas in the brain. Early neonatal hippocampal inactivation alters the devel- , which were differentially expressed in the mutant hippocampus, as a set of biomarkers to characterize the mutants, and performed the statistical clustering of the expression data of these 10 genes in 166 hippocampi of human post-mortem brains. The cluster analysis classified the subjects into two clusters (Cluster A and Cluster B), and Cluster B contained significantly more schizophrenic, schizoaffective and bipolar patients than Cluster A (P = 0.0041, χ 2 test). (B) We compared the gene expression profile between the schizophrenic patients in Cluster B (= schizophrenia-enriched cluster) (n = 19) and the controls with no major psychiatric diagnosis and no CNS-related illness in Cluster A (= control-enriched cluster) (n = 30), using the following criteria; (i) gene expression level was significantly different between two groups (p < 0.01, fold change > 2.0), (ii) present flags were marked in over 75% of hippocampi in at least one of the two groups, (iii) gene expression was not affected by age and gender. We found that 26 probes met these criteria and nearly half of them encoded genes which are known to be involved in neurogenesis or cell-migration/maturation. opment and plasticity of prefrontal cortical circuitry and produces a constellation of behavioral and cellular changes that mimic many aspects of schizophrenia [43][44][45]. Alpha-CaMKII is expressed postnatally and dramatically increases from day 5 after birth [46,47]. Postnatal alterations in DG maturation might trigger changes in intracortical connections, which subsequently cause some of the behavioral phenotypes exhibited by the mutants.

Clustering of Human Post-Mortem Brains by Biomarkers Derived from Alpha-CaMKII+/-Mice
Many of the behavioral abnormalities in alpha-CaMKII+/ -mice are similar to those observed in patients with psychiatric disorders; impairment in working memory is a proposed schizophrenia endophenotype [15], and exaggerated infradian rhythm is a prominent feature of bipolar disorder [48]. Based on these findings, we performed an association analysis of alpha-CaMKII genes with schizophrenia and bipolar disorder. Although there were trends in the associations of several haplotype-tagging single nucleotide polymorphisms with the diseases, our study did not support a major contribution of a genetic mutation of alpha-CaMKII in the susceptibility to either bipolar disorder or schizophrenia in a Japanese population (see Additional file 1, Figure S14, and Additional file 2, Table S5). However, recently, Weinberger's group Total 26 probes were determined as highly significantly affected independently from age and gender in schizophrenic patients of their specific cluster. Neurogenesis-related genes are shown in italics and cell maturation/migration-related genes are in bold. Note that calbindin, a maker for mature neurons in DG, is significantly downregulated in the schizophrenic patients.
reported that a polymorphism in the gene for alpha-CaM-KII is associated with a risk for schizophrenia and that this single nucleotide polymorphism modulates working memory function even in healthy young controls carrying the schizophrenia-associated risk allele [49]. Considering the fact that only a 50% reduction of alpha-CaMKII mRNA caused such a dramatic and qualitative change of the nature of the DG neurons, any genetic or environmental alterations affecting CaMKII signaling pathways could result in a similar change or similar endophenotypes. For example, the NMDA receptor is a major upstream molecule of CaMKII, and its hypofunction, which is implicated in schizophrenia pathophysiology [50], could result in endophenotypes similar to immature DG observed in alpha-CaMKII+/-mice. Schizophrenia, bipolar disorder, and other related psychiatric disorders are considered to be biologically heterogeneous populations, due to the limitation of the current methods of psychiatric diagnosis.
Thus an endophenotype-based analysis would be preferable for establishing a biological underpinning for the classification of psychiatric disorders, rather than an analysis based on the current diagnostic methods [2,6,51].
Statistical clustering of human post-mortem hippocampal samples using the genes/biomarkers derived from the study of alpha-CaMKII+/-mice revealed that human can be classified into two groups, one of which seems to have higher susceptibility to schizophrenia. The two groups differ in their expression of genes related to neurogenesis or neuronal migration/maturation. Though the exact nature of the differences in these two groups is unknown, it is likely that such a difference in gene expression could reflect the differences in development and functions of the DG. In particular, the approximately 60% reduction of calbindin, a marker for mature neurons in DG, in the schizo-enriched cluster is of special interest, suggesting the possibility that an "immature DG" might commonly exist in human. Recently, it was reported that Disc1, a susceptibility gene for schizophrenia, is highly expressed in DG in adulthood [52,53] and is important for organizing newly generated as well as mature neurons and for regulating the neuronal integration in the DG, which may contribute to the pathophysiology of psychiatric disorders conferred by this gene [54,55]. "Immature DG", that is associated with dramatic functional disturbance of DG, may render higher susceptibility to schizophrenia and/or other related psychiatric disorders. Altered CaMKIIrelated signaling or any other genetic/environmental factors that might cause a similar or equivalent endophenotype in the DG could affect susceptibility to schizophrenia or its related psychiatric disorders. Once reliable endophenotypes based on biologically well-defined pathophysiology, as exemplified by "immature DG", are established, diagnostic clinical criteria and classification of psychiatric disorders would be re-organized. A clear disadvantage of our endophenotype-based approach is that we don't have any methods to identify immature DG in living human at present. However, this problem would be solved in the near future, considering the remarkable advances in molecular and functional non-invasive imaging techniques. The products of the genes that were differentially expressed between the two groups in human post-mortem hippocampus and/or between the two genotypes of the mutant mice could also be utilized for diagnosing or subtyping psychiatric patients, if a small chemical probe is developed that can selectively recognize the gene products. Such an endophenotype-based diagnosis would lead to better treatment strategies with appropriate drugs for each disorder subtype.

Conclusion
In summary, we show that alpha-CaMKII heterozygous knockout mice have dysregulated behaviors, including a severe working memory deficit and an exaggerated infradian rhythm. Most neurons in the mutant DG failed to mature, with differential expressions of more than 2000 genes in hippocampus. Transcriptome analysis of human post-mortem brain revealed that biomarkers derived from mutants classified individuals into two clusters, one of which had higher susceptibility to schizophrenia and differentially expressed genes related to neural development, including calbindin, a marker for mature DG neurons. Taken together, we propose "immature DG" as a candidate endophenotype of schizophrenia and other psychiatric disorders ( Figure 6).

Behavioral analysis
Animals and experimental design alpha-CaMKII+/-mice were obtained from Jackson Laboratories (Bar Harbor, Maine). We used heterozygous alpha-CaMKII knockout mice, because it is hard to obtain homozygotes, due to a difficulty of mating between heterozygous male and heterozygous female mice. Mice were housed one per cage in a room with a 12-hr light/dark cycle (lights on at 7:00 a.m.) with access to food and water ad libitum. Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. After the tests, the apparatus were cleaned with super hypochlorous water to prevent a bias due to olfactory cues. All behavioral tests were conducted in a manner similar to those described previously [14,56,57]. All behavioral testing procedures were approved by the Animal Care and Use Committee of Kyoto University Graduate School of Medicine.

Neurological screen
Neurological screen was performed with 9 to 10-wk-old male mice. The righting, whisker touch, and ear twitch reflexes were evaluated. A number of physical features, including the presence of whiskers or bald hair patches, were also recorded.

Neuromuscular strength
Neuromuscular strength was performed with 9 to 10-wkold male mice, and tested with the grip strength test and wire hang test. A grip strength meter (O'Hara & Co., Tokyo, Japan) was used to assess forelimb grip strength.
Mice were lifted and held by their tail so that their forepaws could grasp a wire grid. The mice were then gently pulled backward by the tail with their posture parallel to the surface of the table until they released the grid. The peak force applied by the forelimbs of the mouse was recorded in Newtons (N). Each mouse was tested three times, and the greatest value measured was used for statistical analysis. In the wire hang test, the mouse was placed on a wire mesh that was then inverted and waved gently, so that the mouse gripped the wire. Latency to fall was recorded, with a 60 s cut-off time.

Rotarod test
Motor coordination and balance were tested with the rotarod test. The rotarod test, using an accelerating rotarod (UGO Basile Accelerating Rotarod), was performed by placing 11 to 12-wk-old mice on rotating drums (3 cm diameter) and measuring the time each animal was able to maintain its balance on the rod. The speed of the rotarod accelerated from 4 to 40 rpm over a 5-min period.
Open field test Locomotor activity was measured using an open field test. Open field test was performed with 10 to 11-wk-old male mice. Each mouse was placed in the center of the open field apparatus (40 × 40 × 30 cm; Accuscan Instruments, Columbus, OH). Total distance traveled (in cm), vertical activity (rearing measured by counting the number of photobeam interruptions), time spent in the center, the beam-break counts for stereotyped behaviors, and number of fecal boli were recorded. Data were collected for 120 min.

Light/dark transition test
Light/dark transition test was performed with 9 to 10-wkold male mice, as previously described [58]. The apparatus used for the light/dark transition test consisted of a cage (21 × 42 × 25 cm) divided into two sections of equal size by a partition containing a door (O'Hara & Co., Tokyo, Japan). One chamber was brightly illuminated (390 lux), whereas the other chamber was dark (2 lux). Mice were placed into the dark side and allowed to move freely between the two chambers with the door open for 10 min. The total number of transitions between chambers, time spent in each side, first latency to enter the light side and distance traveled were recorded automatically. Data acquisition and analysis were performed automatically using Image EP software.

Hot plate test
The hot plate test was used to evaluate sensitivity to a painful stimulus. 10 to 11-wk-old mice were placed on a 55.0 (± 0.3)°C hot plate (Columbus Instruments), and latency to the first hind-paw response was recorded. The hind-paw response was defined as either a foot shake or a paw lick.

Startle response/prepulse inhibition tests
Startle response/prepulse inhibition tests were performed with 11 to 12-wk-old male mice. A startle reflex measurement system was used (O'Hara & Co., Tokyo, Japan) to measure startle response and prepulse inhibition. A test session began by placing a mouse in a plastic cylinder where it was left undisturbed for 10 min. White noise (40 ms) was used as the startle stimulus for all trial types. The startle response was recorded for 140 ms (measuring the response every 1 ms) starting with the onset of the prepulse stimulus. The background noise level in each chamber was 70 dB. The peak startle amplitude recorded during the 140 ms sampling window was used as the dependent variable. A test session consisted of six trial types (i.e., two types for startle stimulus only trials, and four types for prepulse inhibition trials). The intensity of the startle stimulus was 110 or 120 dB. The prepulse sound was presented 100 ms before the startle stimulus, and its intensity was 74 or 78 dB. Four combinations of prepulse and startle stimuli were used (74-110, 78-110, 74-120, and 78-120). Six blocks of the six trial types were presented in pseudorandom order such that each trial type was presented once within a block. The average inter-trial interval was 15 s (range: 10-20 s). , a marker for late-stage progenitor cells and immature neurons, and calretinin, a marker for immature neurons, was dramatically increased, whereas the amount of calbindin, a marker for mature neurons in the DG, -positive cells was markedly reduced. Electrophysiological study of the DG neurons showed that input resistance was high and the number of spikes during sustained depolarization was decreased in mutant mice. Furthermore, morphological analysis revealed that dendritic branching and length were decreased in the mutant DG. Collectively, the mutant DG granule cells had many features that are characteristic of immature DG neurons. Red arrows represent the specific changes in the DG of alpha-CaMKII+/-mice. (B) A schematic model of gene-to-behavior pathways in alpha-CaMKII+/-mice. Alpha-CaMKII deficiency leads multiple abnormal gene expression and signal transduction, which causes "immature DG" and impaired function of hippocampus and other brain regions, resulting in abnormal behaviors of alpha-CaMKII+/-mice. Based on the finding that expression changes of genes related to neurogenesis and neural maturation/migration, including calbindin, in hippocampus is associated with higher incidence of schizophrenic patients, "Immature DG" and its equivalent hippocampal functional abnormalities may serve as a promising candidate endophenotype of psychiatric disorders, such as schizophrenia and bipolar disorders.

Social interaction test in a novel environment
Social interaction test in a novel environment was performed with 10 to 11-wk-old male mice. Two mice of identical genotypes that were previously housed in different cages, were placed into a box together (40 × 40 × 30 cm) and allowed to explore freely for 10 min. Social behavior was monitored by a CCD camera, which was connected to a Macintosh computer. Analysis was performed automatically using Image SI software. Total duration of contact, the number of contacts, the number of active contacts, mean duration per contact, and total distance traveled were measured. The number of active contacts was defined as follows. Images were captured at 1 frame per second, and distance traveled between two successive frames was calculated for each mouse. If the two mice contacted each other and the distance traveled by either mouse was longer than 5 cm, the behavior was considered as 'active contact'.

Porsolt forced swim test
Porsolt forced swim test was performed with 11 to 12-wkold male mice. The apparatus consisted of four plastic cylinders (20 cm height × 10 cm diameter). The cylinders were filled with water (23°C) up to a height of 7.5 cm. Mice were placed into the cylinders, and their behavior recorded over a 10-min test period. Data acquisition and analysis were performed automatically, using Image PS software (see 'Image Analysis'). Distance traveled was measured by Image OF software (see 'Image Analysis') using stored image files.

Eight-arm radial maze test
Eight-arm radial maze test was performed with 13 to 14wk-old male mice. Fully-automated eight-arm radial maze apparatuses (O'Hara & Co., Tokyo, Japan) were used. The floor of the maze was made of white plastic, and the wall (25 cm high) consisted of transparent plastic. Each arm (9 × 40 cm) radiated from an octagonal central starting platform (perimeter 12 × 8 cm) like the spokes of a wheel. Identical food wells (1.4 cm deep and 1.4 cm in diameter) with pellet sensors were placed at the distal end of each arm. The pellets sensors were able to automatically record pellet intake by the mice. The maze was elevated 75 cm above the floor and placed in a dimly-lit room with several extra-maze cues. During the experiment, the maze was maintained in a constant orientation. One week before pretraining, animals were deprived of food until their body weight was reduced to 80% to 85% of the initial level. Pretraining started on the 8th day. Each mouse was placed in the central starting platform and allowed to explore and consume food pellets scattered on the whole maze for a 30-min period (one session per mouse). After completion of the initial pretraining, mice received another pretraining to take a food pellet from each food well after being placed at the distal end of each arm. A trial was finished after the mouse consumed the pellet. This was repeated eight times, using eight different arms, for each mouse. After these pretraining trials, actual maze acquisition trials were performed. In the spatial working memory task of the eight-arm radial maze, all eight arms were baited with food pellets. Mice were placed on the central platform and allowed to obtain all eight pellets within 25 min. A trial was terminated immediately after all eight pellets were consumed or 25 min had elapsed. An 'arm visit' was defined as traveling more than 5 cm from the central platform. The mice were confined at the center platform for 5 s after each arm choice. The animals went through one trial per day. For each trial, arm choice, latency to obtain all pellets, distance traveled, number of different arms chosen within the first eight choices, the number of arm revisited, and omission errors were automatically recorded. In the reference memory task of the eight-arm radial maze, one of the eight arms was consistently baited with one food pellet in the food well and a trial was terminated immediately after the one pellet was consumed. Data acquisition, control of guillotine doors, and data analysis were performed by Image RM software (see 'Image analysis').

Locomotor activity monitoring in home cage
Circadian rhythm monitoring in home cage was performed with 18 to 19-wk-old male mice. A system that automatically analyzes the locomotor activity of mice in their home cage was used [8]. The system contains a home cage (29 × 18 × 12 cm) and a filtered cage top, separated by a 13-cm-high metal stand containing an infrared video camera, which is attached to the top of the stand. Each mouse was individually housed in each home cage, and their locomotor activity was monitored for at least 3 mo. Outputs from the video cameras were fed into a Macintosh computer. Images from each cage were captured at a rate of one frame per second, and distance travelled was measured automatically using Image HA software (see 'Image analysis').

Image analysis
The applications used for the behavioral studies (Image LD, Image EP, Image RM, Image FZ, Image SI, and Image HA) were based on the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on the Internet at http://rsb.info.nih.gov/ nih-image/) and ImageJ program http://rsb.info.nih.gov/ ij/, which were modified for each test by Tsuyoshi Miyakawa (available through O'Hara & Co., Tokyo, Japan).

Statistical analysis
Statistical analysis was conducted using StatView (SAS Institute, Cary, NC). Data were analyzed by two-way ANOVA, or two-way repeated measures ANOVA, unless noted otherwise. Values in tables and graphs were expressed as mean ± s.e.m.

Golgi impregnation
Golgi impregnation was performed using FD Rapid Gol-giStain™ kit according to the manufacturer's directions (FD NeuroTechnologies, Inc., Ellicott City, MD). Briefly, the brains of 45-wk old control mice (n = 5) and mutant mice (n = 5) were obtained after cervical dislocation, immersed in the impregnation solution for one week in the dark, and cryoprotected for 48 h. After sectioning (100 μm-thick) and mounting on adhesive-coated slides, sections were stained in a development solution. After the slides containing hippocampal sections were scanned with a light microscope (Zeiss Axioplan2) outfitted with a CCD camera, the resulting images were analyzed by the ImageJ software http://rsb.info.nih.gov/ij/. All slides were coded prior to the quantitative analysis, and the code was broken only after the analysis was completed. To be selected for analysis, Golgi-impregnated neurons had to satisfy the following criteria: (1) dark and consistent impregnation throughout the extent of the dendrites, (2) relative isolation from neighboring impregnated cells, and (3) cell bodies that were located toward the middle of the slice with dendrites lying in the same plane as the cell body. The number of dendritic intersections and dendritic length were measured in successive radial segments of 20 μm distance, using the center of the soma as a reference point (Sholl's analysis). Images of Golgi-impregnated neurons were captured using a Keyence Biozero BZ-8000.

Biochemical assay of monoamine levels
Levels of dopamine, noradrenaline, serotonin, and their metabolites in the brain samples were determined by high-performance liquid chromatography (HPLC) with electrochemical detection [59]. Control mice (n = 9) and mutant mice (n = 9) (10 to 12-wk-old) were killed by decapitation after which their hippocampus, striatum, nucleus accumbens, and medial prefrontal cortex were immediately dissected and frozen in liquid nitrogen. Wet tissue samples were weighed and stored at -80°C until subsequent analysis. Tissues were homogenized in 9 volumes of 0.2 M perchloric acid prior to centrifugation at 20,000 g for 10 min. After the supernatant was neutralized with sodium acetate, the samples were analyzed by HPLC.
Whole-cell recordings were made from granule cells in the dentate gyrus by using the blind whole-cell patch-clamp technique. Current-clamp recordings were made with a pipette filled with a solution composed of (in mM) potassium gluconate 140, HEPES 20, NaCl 8, MgATP 2, Na 2 GTP 0.3, EGTA 0.05 (pH adjusted to 7.2 with KOH). The recording pipette was placed in the middle third of the granule cell layer. Resting membrane potentials were measured immediately after rupture of the patch membrane. Liquid junction potentials were not corrected. Hyperpolarizing currents (4 -16 pA, 400 ms) were injected through the recording pipette to measure the input resistance. Depolarizing currents (400 ms) were injected to evaluate action potential firing properties. The current intensity was increased by 20 to 40 pA steps, and the number of action potentials and the latency of the first action potential were measured.
Field excitatory postsynaptic potentials (EPSPs) arising from mossy fibers (MFs) were recorded from the CA3 region of the hippocampus. Bipolar tungsten stimulating electrodes were placed in the dentate gyrus granule cell layer to activate MFs and a recording glass electrode filled with 2 M NaCl was placed in the stratum lucidum of the CA3 region. Single electrical stimulations were delivered at a frequency of 0.05 Hz unless otherwise specified. An agonist of group II metabotropic glutamate receptors, (2S,2'R,3'R)-2-(2',3'-Dicarboxycyclopropyl)glycine (DCG-IV), which selectively suppresses the MF input, was applied at the end of each experiment. DCG-IV was less effective in mutant mice (92.8 ± 1.0% block in wild-type mice, n = 20; 87.6 ± 0.5% block in mutant mice, n = 23; p < 0.0001). Given changes in the expression level of various genes in these mutant mice, this reduction of the DCG-IV effect might be due to altered expression of metabotropic glutamate receptors and/or molecules involved in downstream signaling. The EPSP amplitude was measured throughout the study following a previously described method unless otherwise specified [60].
In most experiments, the experimenter was blind to the genotype of the mice. All recordings were made using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), filtered at 2 to 10 kHz and stored in a personal computer via a digital interface (digitized at 5 -20 kHz). DCG-IV was purchased from Tocris (Bristol, UK). All values are expressed as means ± s.e.m. Statistical significance was evaluated by two-tailed Student's t-test with a significance level of p less than 0.05.

Microarray analysis
Microarray experiments were performed with 12-wk-old male mice (3 control mice and 3 mutant mice) and 26 to 40-wk old mice (6 control mice and 15 mutant mice). The older mice had been tested in the behavioral test battery, including general health and neurological screen, light/ dark transition test, open field test, elevated plus maze test, social interaction test, rotarod, prepulse inhibition, and Porsolt forced swim test. RNA was isolated by using the TRIzol method (Invitrogen, Carlsbad, CA) from the hippocampus of control mice (n = 9) and alpha-CaM-KII+/-mice (n = 18), followed by purification, using RNeasy columns (Qiagen, Valencia, CA). Double-stranded cDNA was synthesized from the total RNA, and in vitro transcription reaction was then performed on biotinlabeled RNA that was made from the cDNA. Labeled RNA was hybridized with Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA) containing 45101 probe sets, and washed according to the manufacturer's recommendations. The hybridized probe array was then stained with streptavidin-conjugated phycoerythrin, and each GeneChip was scanned by an Affymetrix GeneChip Scanner 3000 (GCS3000). GeneChip analysis was performed with Microarray Analysis Suite version 5.0. All of the genes represented on the GeneChip were globally normalized. Two-way analysis of variance was performed for each probe, and the probes, for which the expression levels were significantly changed (genotype effect, p < 0.05) and not affected by the interaction between genotype and age (p > 0.05), were identified. Expression data and other information used in this paper will be available at the ArrayExpress database (http://www.ebi.ac.uk/microarrayas/aer/, accession number: E-MEXP-962).

Quantitative RT-PCR
RT-PCR was performed to validate the microarray results. Total RNA was isolated from 27 to 29-wk-old control mice and alpha-CaMKII+/-mice. First-strand cDNA was prepared from 2 ug of DNase I-treated total RNA using SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA). The expression of related genes was quantified using the SYBR green reagent (2× SYBR Green PCR Master Mix; Qiagen, Valencia, CA) following the instructions of the manufacturer. Quantitative PCR was performed using DNA Engine Opticon 2 Real-Time PCR Detection System(Bio-Rad, Hercules, CA) with conditions as follows:

Human genetic study Subjects
In the association analyses, two independent samples were examined: for the initial screening, 376 patients with schizophrenia (195 males and 181 females; mean age ± standard deviation (SD) 42.5 ± 14. Sixty-four controls were used as subjects for linkage disequilibrium (LD) evaluation. These subjects were also included in the initial screening scan. Characterization details and psychiatric assessment of these subjects were identical to those published elsewhere [61,62]. All subjects were unrelated to each other and ethnically Japanese.
After the study was described to the subjects, written informed consent was obtained from each subject. This study was approved by the Ethics Committee at Fujita Health University and Nagoya University School of Medicine.

Mutation search
Primer pairs were designed using information from the GenBank sequence (accession number: NT-029289.10) and 23 amplified regions, which covered the coding exon and 5'-flanking region 968 bp upstream. The details are as previously described [63]. Sequences of primer pairs are available on request.

Single nucleotide polymorphism (SNP) selection and Linkage Disequilibrium (LD) evaluation
For the evaluation of LD, we included SNPs from databases (dbSNP, NCBI; and SNPbrowser2.0, Applied Biosystems, Foster City, CA). First we determined 'LD blocks' with the criteria D' > 0.8, using HAPLOVIEW ver 3.0 software [64]. We excluded the minor allele frequencies of SNPs less than 0.05. Next, haplotype-tagging SNPs were selected within each LD block for 90% haplotype coverage using SNPtagger software [65].

SNP genotyping
We used TaqMan assays, primer extension using denaturing high performance liquid chromatography, PCR-RFLP assays, and direct sequencing. Primer pair sequences are available upon request.

Statistical analysis
Genotype deviation from the Hardy-Weinberg equilibrium (HWE) was evaluated by c 2 test (SAS/Genetics, release 8.2, SAS Institute Japan Inc., Tokyo, Japan). Marker-trait association was evaluated allele/genotypewise with c 2 test (SPSS 10.0 J, SPSS Japan Inc.), and haplotype-wise with the log likelihood ratio test (COCAPHASE 2.403 program [66]). Rare haplotypes found in less than 5% of cases and control subjects were excluded from the association analysis to provide greater sensitivity and accuracy. The significance level for all statistical tests was 0.05.

Autoradiography
Frozen brains were obtained from 25-wk-old alpha-CaM-KII+/-mice and control mice, and were cut into 10-mmthick coronal sections with a HM560 cryotome (Carl Zeiss, Oberkochen, Germany). The sections were mounted on slide glass (Matsunami Glass, Osaka, Japan) and stored at -80°C until analyzed. Dopamine D1 and NMDA receptor levels were determined analyzing specific binding of [ 3 H]SCH233090 (1 nM) and [ 3 H]MK801 (5 nM), respectively, to the brain slices using autoradiography. The buffers and incubation times were as described in previous reports [67,68] H]flumazenil were determined by adding 1 mM of butaclamol, 10 mM of nomifensine, 1 mM of fluoxetine, and 10 mM of flunitrazepam, respectively. Following the incubation, the samples were rinsed with ice-cold buffer, and desalted with ice-cold distilled water. The slices were subsequently dried under warm blowing air and exposed to an imaging plate (Fuji Film, Tokyo, Japan) for 10 to 14 days. The imaging plate was subsequently scanned with a BAS5000 system (Fuji Film). Regions of interest (ROIs) were defined on the images using a Multi Gauge ® software (Fuji Film), and densitometric assay for each ROI was performed using autoradiographic [ 3 H]micro-scales (GE Healthcare Bio-Sciences Corp., Piscataway, NJ).

Analysis of c-Fos expression following footshock
For the analysis of c-Fos expression, electric shocks (0.6 mA, 125 V) were delivered to control mice (8 wk-old, n = 4; 13 wk-old, n = 8) and alpha-CaMKII+/-mice (8 wk-old, n = 4; 13 wk-old, n = 6) 10 times through the metal grids in the bottom of a chamber. The duration of each foot shock was 1 s, and the interval between pairs of shocks was 30 s. Two hours after exposure to the last foot shock, mice were perfusion-fixed with 4% PFA and 0.5% picric acid in 0.1 M PBS. The brains were removed and further immersion-fixed in the same fixative at 4°C overnight and 70-μm-thick coronal sections were prepared on a Vibratome. The immunostaining was performed by incubating free-floating sections with rabbit anti-c-Fos polyclonal antibody (Santa Cruz Biotechnology, 1:5000 dilution) at 4°C overnight. After rinse with PBS, the sections were incubated at 4°C overnight with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:300 dilution; Invitrogen), and the fluorescent signals were detected as described above. The number of c-Fos-immunoreactive cells in the specified regions or subdivisions were counted on the confocal images in a blinded manner.

BrdU labeling analyses
Mice (7-wk-old) received four injections of 5-bromo-2deoxyuridine (BrdU) (Sigma, St. Louis, MO), one every 2 h, at 50 mg/kg body weight (10 mg/ml in PBS). Mice were perfusion-fixed with 4% PFA and 0.5% picric acid in 0.1 M PBS 1 d after the BrdU injections. The brains were removed and further immersion-fixed in the same fixative at 4°C for 2 h and 14-μm-thick coronal sections were prepared on a cryostat (Leica). The sections were boiled in 0.01 M citric acid for 10 min and incubated in 2N HCl for 10 min at 37°C, and washed with PBS. The sections were incubated at 4°C for 18 h with mouse BrdU monoclonal antibody (IgG, 1:100 dilution; Becton Dickinson, San Jose, CA). After washing with PBS, the sections were incubated at 4°C for 2 h with Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400 dilution; Invitrogen), and the fluorescent signals were detected as described above. For quantitative analysis, every fourth section (14 mm; throughout the DG in its rostrocaudal extension) was used for counting, and the total cell number was obtained by multiplying the number of counted cells by 4 [75]. The cells were counted in a blind manner.

Electron microscopy
The mice (8 to 13-wk-old) were perfused transcardially with a solution of 2% PFA and 2.5% glutaraldehyde in PBS. The brains were removed and coronal sections (100 mm thick) were prepared on a Vibratome. Sections containing the dorsal hippocampus were osmicated, dehydrated, and embedded in epoxy resin. Ultrathin sections of the hippocampal CA3 region were prepared and stained with lead citrate and uranyl acetate, and observed under a Hitachi H-7000 electron microscope.

Gene expression studies using postmortem brain tissue
The expression data for 166 hippocampal genes from the BioExpress database [76,77] (GeneLogic) were used in this study (see Additional file 2, Table S3). Differential analysis of mouse GeneChip data indicates that 130 mouse probes (n = 3, p < 0.05 [t-test], fold change > 1.5) were affected in mutants, independent of age or experience. Fifty-seven human orthologues corresponding to the 130 mouse probes were present in at least 10% of the 166 hippocampi and were identified as biomarkers. Of the 166 hippocampi, there were 18 from patients with schizophrenia, 2 with bipolar disorder, and 1 with schizoaffective disorder (see Additional file 2, Table S3). Variance analysis of hippocampal expression levels was performed to identify the 10 best genes of the 57 biomarkers so that the 21 hippocampi of patients with psychiatric disorders would be distinguished from the remaining 147 hippocampi (see Additional file 2, Table S4). Cluster analysis of the 166 hippocampi based on the expression levels of the 10 best biomarkers was performed using Spotfire DecisionSite™ For Functional Genomics http:// spotfire.tibco.com/. Of the 166 hippocampi, 44 were obtained from donors with no known history of CNSrelated illness. Of the 44 donors with no CNS-related illness, 28 were over 50 years old and 26 were male. We defined age-and gender-affected probes by differential expression analysis of older (over 50 years old) vs. younger donors with no CNS-related illness and of male vs. female donors with no CNS-related illness, respectively (p < 0.05, fold change > 1.8). For cluster-based differential gene expression analysis, age-and genderaffected probes were eliminated and expression levels of other probes in clustered psychiatric hippocampi were compared with those in the other cluster obtained from donors with no CNS-related illness as specific controls (p < 0.01, fold change > 2.0). In addition, only probes whose present flags were marked in over 75% of hippocampi in at least one of the two groups (case and specific controls) were considered significant.
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