Conditional Pten knockout in parvalbumin- or somatostatin-positive neurons sufficiently leads to autism-related behavioral phenotypes

Disrupted GABAergic neurons have been extensively described in brain tissues from individuals with autism spectrum disorder (ASD) and animal models for ASD. However, the contribution of these aberrant inhibitory neurons to autism-related behavioral phenotypes is not well understood. We examined ASD-related behaviors in mice with conditional Pten knockout in parvalbumin (PV)-expressing or somatostatin (Sst)-expressing neurons, two common subtypes of GABAergic neurons. We found that mice with deletion of Pten in either PV-neurons or Sst-neurons displayed social deficits, repetitive behaviors and impaired motor coordination/learning. In addition, mice with one copy of Pten deletion in PV-neurons exhibited hyperlocomotion in novel open fields and home cages. We also examined anxiety behaviors and found that mice with Pten deletion in Sst-neurons displayed anxiety-like behaviors, while mice with Pten deletion in PV-neurons exhibited anxiolytic-like behaviors. These behavioral assessments demonstrate that Pten knockout in the subtype of inhibitory neurons sufficiently gives rise to ASD-core behaviors, providing evidence that both PV- and Sst-neurons may play a critical role in ASD symptoms.


Introduction
Inhibitory neurons are highly impacted in autism spectrum disorder (ASD) [1][2][3], a neurodevelopmental condition characterized by symptoms of difficulty in communication, deficits in social interaction, and the presence of restricted/repetitive behaviors [4].Soma-targeting parvalbumin (PV)-expressing neurons (PV-neurons) and dendritic-targeting somatostatin (Sst)expressing neurons (Sst-neurons) are two prominent subtypes of inhibitory neurons in the cortex [5].Studies of postmortem human brain tissues have demonstrated aberrant inhibitory neurons, mainly PV-neurons, in the brains of individuals with autism [6][7][8][9].Disrupted inhibitory neurons have also been extensively reported in animal models for autism [10][11][12].However, the contribution of each subtype of inhibitory neurons to autism symptoms is largely unknown.Thus, evaluating the role of PV-neurons and Sst-neurons in autism behaviors is crucial for the development of treatments.
We used the Cre/loxp recombination system to generate mice with Pten knockout in PV-neurons or Sstneurons, and conducted a battery of behavioral tests to examine autism-related behavioral phenotypes in these mice.We found that conditional knockout Pten in PVneurons or Sst-neurons is sufficient to result in autismcore symptoms, including social deficits and repetitive behaviors.We also showed that Pten in PV-neurons and Sst-neurons is crucial for motor coordination and learning.Finally, deletion of Pten in PV-neurons induces hyperlocomotion and anxiolytic-like behaviors whereas deletion of Pten in Sst-neurons causes anxiety-like behaviors.Collectively, Pten mutation in PV-neurons and Sstneurons results in autism-related behavioral phenotypes.

Deletion of Pten in PV-neurons or Sst-neurons leads to social deficits
First, we examined the effect of conditional Pten knockout in parvalbumin-expressing neurons (PV-neurons) on social behaviors.We specifically deleted Pten in PV-neurons by crossing PV-Cre mice with Pten flox mice to generate PV-Cre +/+ /Pten fl/+ mice (See Methods for details).Using these mice as breeders, we obtained offspring littermates with three genotypes, PV-Cre +/+ /Pten +/+ (PV-Pten-WT), PV-Cre +/+ /Pten fl/+ (PV-Pten-Het), and PV-Cre +/+ /Pten fl/fl (PV-Pten-KO).These three types of mice all have homozygous Cre but different copies of Pten in PV-neurons.Using immunohistochemistry and Western blot, we confirmed that Pten was specifically reduced and deleted in PV-positive neurons of PV-Pten-Het and PV-Pten-KO mice, respectively (Additional file 1: Figure S1).Sociability and social novelty preference in these mice were assessed using modified threechamber social tests (Fig. 1a).Firstly, we confirmed that there was no pre-existing side preference in the PV-Pten mice (Additional file 1: Figure S3A).As shown in Fig. 1b, single or two copies of Pten deletion in PV-neurons did not change the social approach behaviors.All three genotypes of PV-Pten mice spent more time with social partners than with objects in the sociability test.The preference index was not different among the three groups of PV-Pten mice (Fig. 1b).In contrast, knockdown or knockout of Pten in PV-neurons impaired the social novelty preference.In the social novelty test, only PV-Pten-WT mice spent more time interacting with novel social partners (Fig. 1c).The preference index for social novelty decreased in PV-Pten-Het and PV-Pten-KO mice compared to that in PV-Pten-WT mice (Fig. 1c).

Deficits of locomotion and motor coordination and increased repetitive behaviors in PV-Pten and Sst-Pten mice
We then examined the contribution of Pten mutations in PV-and Sst-neurons to deficits in locomotor behaviors.30-min open field tests were performed in PV-Pten mice (Fig. 2a).PV-Pten-Het but not -KO mice traveled significantly longer distances compared to PV-Pten-WT mice (Fig. 2b).When examined in 5-min intervals, the distance moved in PV-Pten-WT and -Het mice declined slightly over the 30-min testing period, and PV-Pten-Het mice consistently traveled longer distances in each interval.PV-Pten-KO mice maintained the same rate of activity over the 30-min testing time (p = 0.903, Pearson correlation test), but no statistical difference compared to PV-Pten-WT mice (Fig. 2c).We examined the locomotor activity in home cages as well.Consistent to the result in the open-field tests, PV-Pten-Het mice but not PV-Pten-KO mice exhibited increased locomotor activity (Fig. 2d  and e).Thus, PV-Pten-Het mice exhibited elevated locomotor activity both in the novel open field and home cages.In Sst-Pten mice, the locomotor activity was not different among genotypes (Fig. 2f ), and was confirmed by measurements across 5-min intervals (Fig. 2g).Sst-Pten mice also performed normally in the home-cage activity test (Fig. 2h and i).
We next examined self-grooming during the open field test for repetitive behaviors.PV-Pten-KO mice exhibited an increase of grooming bouts (Fig. 3a) and spent more time in self-grooming in the open field (Fig. 3b).Similarly, the number of grooming bouts and total time of self-grooming were increased in Sst-Pten-KO mice (Fig. 3c and d).These data suggest that deletion of Pten in PV-neurons or Sst-neurons induces repetitive behaviors, a core-domain of autism behaviors.
We further evaluated the motor coordination/learning in PV-Pten mice using the rotarod test (Fig. 4a).Mice were trained on an accelerating rotarod for 6 trials over two days (3 trials per day).The latency to fall was significantly reduced in PV-Pten-Het and PV-Pten-KO mice over the 6-trial training (Fig. 4b).Particularly, motor coordination was severely impaired in PV-Pten-KO mice.They were barely able to stay on the stationary rotarod (Fig. 4b).We next analyzed the learning index (ratio of latency to fall between the 6th trial and the 1st trial) and found that knockout of Pten in PV-neurons impaired motor learning in the rotarod test (Fig. 4c).Similarly, knockout of Pten in Sst-neurons impaired rotarod performance and motor learning as well (Fig. 4d and e).Collectively, knockdown or knockout of Pten in PV-neurons or Sst-neurons impairs motor coordination and learning.
We then conducted marble burying tests in PV-Pten and Sst-Pten mice (Fig. 4f ).PV-Pten-KO mice buried significantly fewer marbles than PV-Pten-WT mice did (Fig. 4g), but there was no difference in the marble burying task among the three genotypes of Sst-Pten mice (Fig. 4h).Since the burying of marbles entails physical motor activity, reduced marble burying activity in PV-Pten-KO mice may be mainly resulted from impaired motor coordination in this type of mice (Fig. 4b).

Anxiolytic-like behavior in PV-Pten-KO mice and anxiety-like behavior in Sst-Pten-KO mice
Finally, we examined anxiety-like behaviors in mice with Pten deletion in inhibitory neurons using elevated plus maze tests (Fig. 5a) and open field tests (Fig. 5b).In the elevated plus maze, PV-Pten-KO mice spent increased percentage of time in the open arms and decreased percentage of time in the closed arms, compared to PV-Pten-WT and PV-Pten-Het mice (Fig. 5c and d In contrast, Sst-Pten-KO mice showed a reduced percentage of time in the open arms and an increased percentage of time in the closed arms (Fig. 5g and h).We did not observe any difference of time spent in the center or corner areas among the three genotypes of Sst-Pten mice (Fig. 5i and j).Thus, we concluded that Pten knockout in PV-neurons induces anxiolytic-like behavior while Pten knockout in Sst-neurons evokes anxiety-like behavior.

Discussion
Our study revealed that conditional deletion of Pten in either PV-neurons or Sst-neurons results in autismlike core symptoms, including reduced social novelty preference and presence of repetitive behaviors (selfgrooming).In addition, conditional Pten knockout in PV-neurons causes differences in behaviors that are characteristic symptoms often observed in autism, such as hyperactivity and impairment in motor coordination/ learning.Similarly, conditional Pten knockout in Sst-neurons gives rise to disrupted motor coordination/learning.Interestingly, PV-Pten-KO mice displayed anxiolytic-like behaviors, whereas Sst-Pten-KO mice showed anxietylike behaviors.These findings provide the behavioral evidence that Pten mutation in PV-neurons or Sst-neurons sufficiently induces ASD-related behaviors.
The mice used in this study all had homozygous Cre and different copies of Pten in PV-neurons or Sst-neurons.Reduced somatostatin expression in homozygous Sst-Cre mice has been previously reported [31], which might potentially impact behavioral performance.We compared behaviors in PV-Pten-WT mice and Sst-Pten-WT mice with those in Pten fl/fl mice, and found that PV-Pten-WT and Sst-Pten-WT mice did not exhibit any difference in most behavioral tests, except that Sst-Pten-WT mice displayed increased time spent in the center of the open field test (Additional file 1: Figure S4).Since Cre expression is just the background for all genotypes of mice used here, our conclusion in this study should not be affected by the change of baseline behavioral performance in Sst-Pten-WT mice.We were also wondering whether the different ages of mice performed similarly in the behavioral tests.By performing correlation tests, we found that there was no relationship between the age and behavioral performance in all behavioral tests (Additional file 1: Figures S5 and S6).Additionally, the prevalence of autism is different in sex [32] and our previous study in animal models also showed the sex difference in behaviors of Slit3-KO mice [33].However, we did not found any sex effect in the behavioral phenotypes of PV-Pten or Sst-Pten mice (Additional file 1: Figure S7 and S8).
Mice with deletion of several other genes (Shank2, mGluR5 and Lom4) in PV-neurons exhibit autism-related behaviors of varying severity [34][35][36], supporting that the function of PV-neurons is an important determinant of autism behaviors.Autism-relevant behavioral phenotypes are also reported in mice with conditional knockout of MeCP2 (Rett syndrome) or Scn1a (Dravet syndrome) in PV-neurons but not Sst-neurons [37,38].In our results, conditional knockout of Pten in either PVor Sst-neurons resulted in autism-like core behaviors, further suggesting that Pten plays an important role in the function of these two subtypes of neurons.Three-chamber social tests are commonly used to examine sociability and social novelty preference in mice [39].The sociability test is to assess the tendency of initiating social contact and remaining proximal to an unfamiliar conspecific, whereas the social novelty test is to evaluate the tendency of approaching novel mice, the discrimination between familiar and novel mice, and the recognition/memorization of the mouse that has interacted before [40].PV-Pten and Sst-Pten mice exhibited deficits mainly in social novelty preference.Multiple brain regions could be involved in the social deficits observed in these mice [41].The prefrontal cortex (PFC) is one of such brain structures that are well established for social behaviors [42].PV-neurons and Sst-neurons in medial prefrontal cortex (mPFC) specifically respond to social stimuli [43,44].Social-evoked responses in PV-neurons are diminished in Cntnap2-KO mice which display deficits in sociability [43].Consistently, optogenetic activation of PV-neurons in mPFC rescues deficits of sociability and social novelty preference in autism mouse models [43,45].However, evidence supporting a relationship between PV-neurons in mPFC and social deficits is not unequivocal [46].
The hippocampus is another brain region which is involved in social interaction, particular social memory, a process that is critical for social novelty preference [47,48].PV-neurons in the ventral hippocampus (vHipp) exhibited increased activity to novel social partners compared to familiar ones, and inactivation of PV-neurons but not Sst-neurons in vHipp disrupts social novelty preference [49].Conversely, another study reported that knockdown Sst but not PV in vHipp disrupted social interaction [50].Our data, however, showed that both PV-and Sst-neurons are important for social novelty preference, indicating that multiple brain regions might contribute to this type of social behaviors.
The cerebellum was recently implicated in social behaviors [51,52].PV and Sst are expressed in Purkinje cells (PC) of the cerebellum [53][54][55].Abnormal PC function has been linked to social deficits in mice with conditional deletion of autism-risk genes in PCs [56][57][58][59].However, conditional Pten knockout in Purkinje cells results in autism-related behaviors, such as less self-grooming and reduced sociability [28], different phenotypes to those exhibited in PV-Pten-KO mice (increased self-grooming and reduced social novelty preference).This discrepancy may be explained by the fact that Cre-recombinase activity in PV-Cre mice is not only present in Purkinje cells but also in cerebellar interneurons [53,60], indicating that cerebellar interneurons may contribute to behavioral phenotypes in PV-Pten-KO mice as well.In addition, other brain regions, such as the amygdala [61,62], ventral tegmental area (VTA)-to nucleus accumbens (NAc) projection [63], and the striatum [64,65] all have been reported to be involved in social behaviors.
Mutation of Pten in PV-neurons may contribute to hyperactivity, a common comorbidity of autism [66].However, only PV-Pten-Het mice exhibited hyperactivity in the open field test.Both PV-Pten-KO mice and Sst-Pten-KO mice displayed impaired motor coordination/learning in accelerating rotarod tasks and increased repetitive behaviors (self-grooming) in open field tests.Especially, PV-Pten-KO mice were barely able to stay on the rotarod.This severe deficit of motor coordination in PV-Pten-KO mice may mask the hyperactivity resulting from the Pten knockout in PV-neurons.The cerebellum and striatum, two brain structures critical for motor coordination/learning and repetitive behaviors [67][68][69], may account for these behavioral phenotypes in PV-Pten and Sst-Pten mice.Additionally, Pten knockout in Purkinje cells results in impaired motor coordination/ learning [28], and inhibition of PCs in cerebellar right crus I results in repetitive behaviors [59].Moreover, Pten regulates striatal dopamine signaling [70,71].All these lines of evidence indicate that motor deficits and repetitive behaviors in PV-Pten and Sst-Pten mice may result from the functional change of PV-and Sst-neurons in the cerebellum and/or striatum; however, further investigations are needed.
Our results showed that PV-Pten-KO mice exhibited anxiolytic-like behaviors while Sst-Pten-KO mice displayed anxiety-like behaviors.Activation of PV-positive neurons in the dentate gyrus results in anxiolytic behaviors [72].Interestingly, PV activity in the nucleus accumbens shell (sNAc) negatively correlates with the time of open-arm exploration in EPM test [73].The mechanisms underlying the differential effects of Pten knockout in these two subtypes of neurons on anxiety-like behaviors are not clear.
At the synaptic level, conditional knockout Pten from GABAergic cortical interneurons increases the synaptic input onto interneurons [74] and the inhibitory output onto glutamatergic neurons [22,75].Conversely, a single copy of Pten deletion from PV-neurons impairs the formation of perisomatic inhibition [76].Though the underlying synaptic mechanisms in PV-Pten-KO and Sst-Pten-KO mice need further investigation, our results provide behavioral evidence that deletion of Pten in either subtype of inhibitory neurons elicits autismrelated phenotypes, suggesting a potential benefit from therapeutic strategies targeting either PV-neurons, Sstneurons, or both subtypes, depending on specific alterations in behavioral phenotype.

Animals
Pten fl/+ , Sst-Cre +/-and PV-Cre +/-mice were purchased from Jaxson Laboratory (Stock #: 006440, 013044 and 008069, respectively).All these strains were maintained on C57BL6/J background.We crossed Pten fl/fl mice with Sst-Cre +/+ mice to generate Sst-Cre +/-/Pten fl/+ mice which were then backcrossed with Sst-Cre +/+ mice to generate Sst-Cre +/+ /Pten fl/+ breeders.By crossing the breeders we obtained three types of experimental mice, Sst-Cre +/+ /Pten +/+ (Sst-Pten-WT), Sst-Cre +/+ /Pten fl/+ (Sst-Pten-Het) and Sst-Cre +/+ /Pten fl/fl (Sst-Pten-KO) mice.They were all homozygous for Cre but expressed two, one or no copy of Pten in Sst neurons.Using the same breeding strategy, we generated PV-Cre +/+ /Pten fl/+ breeders and their offspring, PV-Cre +/+ /Pten +/+ (PV-Pten-WT), PV -Cre +/+ /Pten fl/+ (PV-Pten-Het) and PV-Cre +/+ /Pten fl/fl (PV-Pten-KO) mice.Both male and female littermates at the age of 4-8 weeks were used for behavioral assessments.There was no significant correlation between the behavioral performance and ages in PV-Pten or Sst-Pten WT mice (Additional file 1: Figures S5  and S6).No significant sex effects were observed (Additional file 1: Figures S7 and S8).Age and sex-matched C57BL/6 mice (purchased from Veterinary Resources at the University of Maryland School of Medicine) were used as social partners in modified three-chamber social tests.All mice were housed 2-5 per cage in ventilated racks in a temperature-and humidity-controlled animal room on a 12 h light/dark cycle with lights on from 07:00 to 19:00 and cared by the AAALAC accredited program of the University of Maryland School of Medicine.Autoclaved rodent chow and water were available ad libitum.The experimental protocol was approved by the Institutional Animal Care and Use Committees at the Hussman Institute for Autism and the University of Maryland School of Medicine.

Immunohistochemistry
Animals were anesthetized with isoflurane and perfused transcardially with 0.9% saline solution.Brains were fixed in 4% paraformaldehyde.Double immunohistochemistry with either anti-parvalbumin made in guinea pig (1:3000 dilution; Swant; Catalog # GP72) or anti-somatostatin made in rat (1:200 dilution; MilliporeSigma Cat.# MAB354), and anti-PTEN made in rabbit (1:250 dilution; Cell Signaling; Catalog # 9559T) were performed as described [77] in coronal sections of 40 μm obtained with a vibratome (Leica VT 1000S).Appropriate Alexa fluor conjugated antibodies (Invitrogen) were used to detect primary antibodies.A negative control with normal goat serum in place of primary antibodies was performed on adjacent sections.Images were obtained with LSM 780 (Zeiss) confocal microscope.Imaging conditions were maintained over the different genotypes within each strain.Cells were counted in cortical layers III-IV (PV + cells) or V-VI (Sst + cells) from matching sections (Bregma − 1.7 mm) of three mice per genotype.
PTEN expression levels were quantified by measuring the fluorescence intensity in parvalbumin or somatostatin positive cells with Fiji image analysis package [78].
After background subtraction, fluorescence intensities were normalized by the average PTEN fluorescence intensity in WT mice.

Behavioral assessments
Following procedures established in our previous work [33], a series of behavioral assessments were conducted.Due to poor reproductive performance of mutant mice, multiple behavioral assessments were performed on the same groups of mice.Each mouse was tested for only one behavioral assessment per day.The behavioral tests were performed in the order of potential stress elicited from the tasks during the tests: open field test, elevated plus maze test, modified three-chamber social test, marble burying task, and rotarod test.A large cohort of mice were used for all behavioral tests in the battery, except that a few mice died suddenly before the completion of the whole battery of tests.In addition, a second cohort of Sst-Pten mice were used for the social test only to balance the side preference, and a third cohort of mice were used for the home-cage activity test.Animals' activity during the behavioral assessments was recorded by a Logitech C920 HD Pro Webcam, with the Ethovision software (Noldus, RRID: SCR_002798) used for further offline analysis.All experiments were conducted during the light cycle with room lights at around 80 lx except for the elevated plus maze (around 8 lx).All areas were cleaned with 70% ethanol between test sessions, including areas untouched by mice.

Open field test
Mice were placed in the center of an open field apparatus (40 × 40 cm) and allowed to explore the field for 30 min.The movement was tracked using the Ethovision XT software.Distance moved and time spent in the center area (20 × 20 cm, square) and corner area (15 × 15 cm, triangle) were further analyzed.Self-grooming behaviors were also analyzed from the open field activity using mouse behavior recognition (Detection settings for each mouse: posture between 70 and 90%, probability higher than 75%) in the Ethovision XT.

Home-cage activity test
Home-cage activity was recorded and analyzed using Ethovision XT software.Mice were individually housed in a clean cage for at least 24 h for acclimation.Mouse behaviors were recorded in its home cage with the filter top removed for 30 min.The distance moved was determined by off-line analyses of the video recordings.

Elevated plus maze test
The

Modified three-chamber social test
We used a modified apparatus to examine the social behaviors [79].The apparatus [40 (H) × 60 (L) × 20 (W) cm] was divided into three chambers (20 cm long for each chamber) made from white Plexiglass.The side chambers and the center chamber were separated by white Plexiglass walls with a small gate (10 cm) in the middle.Square interaction areas (10 × 10 cm) in each side chamber was formed by the gate and a three-sided fence made from clear Plexiglass (Fig. 1a).Test mice entered these two areas to interact with social partners.The two side chambers were covered by a transparent Plexiglass sheet during the three-session test.All three sessions were performed consecutively on one day.In the first session (habituation), the test mouse was placed in the center chamber and allowed to explore the chamber for 10 min.At the end of the habituation session, the test mouse was gently guided back to the middle of the center chamber, and the small gates were blocked.During the second session, which was to test the sociability, three novel C57BL/6 mice and three objects were placed in one of the two side chambers, respectively.The side chamber for social partners or objects was randomly assigned by flipping a coin.The test mouse was allowed to explore the center chamber for 10 min.After that, the test mouse was guided back to the middle of the center chamber again.In the third session, which was to test the social novelty preference, the objects were replaced by another set of three novel C57BL/6 mice, and the test mouse was allowed to explore the chamber for another 10 min.The duration that the animal spent in the interaction areas during each session was scored using the EthoVision XT software (Noldus).The preference index was calculated as the duration exploring the novel mice/ the total duration exploring the objects (or familiar mice) and the novel mice.

Marble burying task
Twelve glass marbles (3 × 4 arrangement) were placed on the surface of the bedding (5 cm deep) in standard mouse cages.The cages were covered with filter top during the test.Each test mouse was placed in the cage and allowed to explore for 30 min.The number of buried marbles was counted manually.The marble was checked from side of the cage.If the marble was buried more than 2/3 deep, it was counted as buried.

Rotarod test
The two-session rotarod test was conducted on an accelerating rotarod (Panlab, Harvard Apparatus) over two days.Mice were allowed to become acclimated to the stationary rod for 60 s on the first day.Each test session consisted of three trials with 15-min inter-trial intervals (ITI).During each trial, mice were placed on the rotating rod (4 rpm) facing away from the direction of rotation.The rotation speed was accelerated from 4 to 40 rpm over 5 min.If the mouse fell off the rod within 10 s, the trial was repeated after 15 min.The latency to fall was recorded as the time delay between the start of the trial and the moment when mice fell off the rod or made a complete revolution on the rod.

Statistical analysis
Data was analyzed with SPSS (v.20) and GraphPad Prism 9 Software.All datasets were tested for normality using the Shapiro-Wilk test.For datasets with normal distribution, two-tailed t-test was used for comparisons between two groups, and two-way ANOVA with repeated measures in one factor (rmANOVA) or one-way ANOVA was used for comparisons among three groups.Tukey's post hoc test was used for multiple comparisons after ANOVA test.For datasets with non-normal distribution, Wilcoxon matched-pairs signed rank test (Wilcoxon test) was used for comparisons between two groups (paired), and Kruskal-Wallis test with Dunn's post hoc test was used for comparisons among three groups.One sample t-test or Wilcoxon signed-rank test was used for comparing the mean (or median) of a dataset with a hypothetical number.p-value < 0.05 was considered to be statistically significant.

Fig. 5
Fig. 5 PV-Pten-KO mice showed anxiolytic behaviors while Sst-Pten-KO mice displayed anxiety-like behaviors, compared to their WT mice.a The schematic diagram of the elevated plus-maze test (EPM).b The schematic diagram of the center (blue) and corner area (orange) in the open field test.c PV-Pten-KO mice spent more percentage of time in the open arms compared to PV-Pten-WT and PV-Pten-Het mice (WT: n = 26, Het: n = 17, KO: n = 24; F (2,64) = 7.373, p = 0.001, one-way ANOVA; KO vs. WT: p = 0.005, Tukey's post hoc test).d PV-Pten-KO mice spent less percentage of time in the closed arms compared to PV-Pten-WT and PV-Pten-Het mice (F (2,64) = 5.310, p = 0.007, one-way ANOVA; KO vs. WT: p = 0.023, Tukey's post hoc test).e, f In the open field test, PV-Pten-KO mice spent more time in the center area (e) and less time in the corner area (f), compared to PV-Pten-WT mice (WT: n = 25, Het: n = 17, KO: n = 26; Center: p = 0.024, one-way ANOVA; KO vs. WT: p = 0.023, Tukey's post hoc test; Corner: p = 0.016, Kruskal-Wallis test; KO vs. WT: p = 0.009, Dunn's post hoc test).g Sst-Pten-KO mice spent less percentage of time in the open arms compared to Sst-Pten-WT mice (WT: n = 11, Het: n = 16, KO: n = 17; F (2,34) = 3.984, p = 0.028, one-way ANOVA; KO vs. WT: p = 0.026, Tukey's post hoc test).h Sst-Pten-KO mice spent more percentage of time in the closed arms compared to Sst-Pten-WT mice (F (2,34) = 3.596, p = 0.038, one-way ANOVA; KO vs. WT: p = 0.031, Tukey's post hoc test).i, j In the open field test, there was no difference in time spent in the center (i) or corner area (j) among the three genotypes of Sst-Pten mice (WT: n = 12, Het: n = 16, KO: n = 19; Center: p = 0.851, Kruskal-Wallis test; Corner: p = 0.502, Kruskal-Wallis test).Circles represent data from individual animals, and bar graphs indicate mean + SEM.*: significant; n.s.: not significant elevated plus maze was composed of two open arms (30 × 5 cm) and two closed arms (30 × 5 cm) with 15 cm wall height and 50 cm above the floor.Mice were individually placed in the center of the maze and facing one of the open arms.They were allowed to explore the open and closed arms of the maze for 10 min.The duration of time in arms was recorded.The percentage of time in open arms (or closed arms) was calculated as the duration in open arms (or closed arms)/total exploring duration.