Treatment of intermittent hypoxia increases phosphorylated tau in the hippocampus via biological processes common to aging
- Sosuke Yagishita†1, 5Email author,
- Seiya Suzuki†1, 2,
- Keisuke Yoshikawa1,
- Keiko Iida3,
- Ayako Hirata3,
- Masahiko Suzuki1, 2,
- Akihiko Takashima4,
- Kei Maruyama1,
- Akira Hirasawa3Email author and
- Takeo Awaji1
© The Author(s). 2017
Received: 7 September 2016
Accepted: 15 December 2016
Published: 5 January 2017
Sleep-disordered breathing produces cognitive impairments, and is possibly associated with Alzheimer disease (AD). Intermittent hypoxia treatment (IHT), an experimental model for sleep-disordered breathing, results in cognitive impairments in animals via unknown mechanisms. Here, we exposed mice to IHT protocols, and performed biochemical analyses and microarray analyses regarding their hippocampal samples. In particular, we performed gene ontology (GO)-based microarray analysis to elucidate effects of IHT on hippocampal functioning, which were compared with the effects of various previously-reported experimental conditions on that (ref. Gene Expression Omnibus, The National Center for Biotechnology Information). Our microarray analyses revealed that IHT and aging shared alterations in some common GO, which were also observed with kainic acid treatment, Dicer ablation, or moderate glutamate excess. Mapping the altered genes using the Kyoto Encyclopedia of Genes and Genomes PATHWAY database indicated that IHT and aging affected several pathways including “MAPK signaling pathway”, “PI3K-Akt signaling pathway”, and “glutamatergic synapse”. Consistent with the gene analyses, in vivo analyses revealed that IHT increased phosphorylated tau, reflecting an imbalance of kinases and/or phosphatases, and reduced proteins relevant to glutamatergic synapses. In addition, IHT increased phosphorylated p70 S6 kinase, indicating involvement of the mammalian target of rapamycin signaling pathway. Furthermore, IHT mice demonstrated hyperactivity in Y-maze tests, which was also observed in AD models. We obtained important data or something from the massive amount of microarray data, and confirmed the validity by in vivo analyses: the IHT-induced cognitive impairment may be partially explained by the fact that IHT increases phosphorylated tau via biological processes common to aging. Moreover, as aging is a major risk factor for AD, IHT is a novel model for investigating the pathological processes contributing to AD onset.
KeywordsSleep-disordered breathing Intermittent hypoxia Gene ontology-based microarray analysis Alzheimer disease Tau
Sleep-disordered breathing (SDB) is characterized by recurrent arousals from sleep and intermittent hypoxemia. Associations have been suggested between SDB and various disorders, including hypertension [1, 2], cardiovascular disease , diabetes , and dementia such as Alzheimer disease (AD) [5–7]. Patients with SDB often have cognitive impairments, which are associated with intermittent hypoxia, but not with sleep fragmentation or duration of sleep [8–10]. Thus, intermittent hypoxia treatment (IHT) has been used as an experimental animal model for SDB [11–23]. Consistent with previous clinical studies, rats or mice exposed to IHT, but not to sustained hypoxia , demonstrated memory impairment [16, 21–23], which was attenuated by exogenous administration of growth hormone or erythropoietin [12, 23]. However, the mechanisms of IHT-induced cognitive impairments have not been elucidated. In the present study, in order to find a clue to the mechanisms, we aimed to understand the effects of IHT in the hippocampus, which has crucial roles in learning and memory.
Various complex pathways are involved in cognitive impairments, which are often associated with aging and dementia such as AD , therefore, we first performed a microarray analysis. The resulting data were subjected to a principal component analysis (PCA), and gene ontology (GO)-based microarray analysis according to a previous report . We analyzed not only our experimental data, but also various other data published in Gene Expression Omnibus (GEO: The National Center for Biotechnology Information), in relation to measure of hippocampal functioning. Comparing the massive amount of microarray data led to successful identification of several experimental models that shared gene alterations common with our IHT model. Next, we mapped the altered genes using the Kyoto Encyclopedia of Genes and Genomes (KEGG) PATHWAY database, and investigated several pathways that may be affected by IHT. Finally, we confirmed the insights obtained from in silico analyses by performing in vivo analyses, with findings indicating that IHT increases level of phosphorylated tau (P-tau).
C57BL/6J male mice were purchased from Tokyo Laboratory Animals Science Co., Ltd. (Tokyo, Japan), and began experiments when they were at 10-weeks-old. We also prepared mice reared for 12 months, in order to perform the microarray analysis. Animals were handled according to procedures approved by the Animal Care and Use Committee of Saitama Medical University.
For immunoblots of total tau, we used rabbit polyclonal anti-tau, JM . For immunoblots of phosphorylated tau at each epitope, we used the following antibodies: mouse monoclonal AT270 (Innogenetics, Gent, Belgium), which recognizes pThr181; rabbit polyclonal anti-pSer199 (Biosource, Camarillo, CA); mouse monoclonal AT8 (pSer202, pThr205) (Innogenetics); rabbit polyclonal anti-pThr212 (Biosource); rabbit polyclonal anti-pSer214 (Invitrogen, Carlsbad, CA); mouse monoclonal AT180 (Innogenetics), which recognizes pThr231; rabbit polyclonal anti-pSer396 (Biosource); rabbit polyclonal anti-pSer400 (Sigma-Aldrich, St. Louis, MO); rabbit polyclonal anti-pSer404 (Millipore, Billerica, MA). For immunoblots for other proteins, we used the following antibodies: mouse monoclonal anti-VGluT1 (Millipore); mouse monoclonal anti-synaptophysin (Sigma-Aldrich); rabbit polyclonal anti-GluA1 (Synaptic Systems, Goettingen, Germany); mouse monoclonal anti-GluA2 (Millipore); mouse monoclonal anti-PSD-95 (BD Transduction Laboratories, San Jose, CA); rabbit polyclonal anti-PICK1 (Thermo Fishier Scientific, Waltham, MA); mouse monoclonal anti-α-Tubulin (Sigma-Aldrich); mouse monoclonal anti-GSK-3β (BD Transduction Laboratories); rabbit polyclonal anti-phospho-GSK-3β (Ser9) (Cell Signaling Technology, Danvers, MA); mouse monoclonal anti- phospho-GSK-3β (Tyr216) (BD Transduction Laboratories); rabbit polyclonal anti-PP2A A subunit, B subunit, and C subunit (Cell Signaling Technology); rabbit polyclonal anti-p70 S6 kinase (Cell Signaling Technology); rabbit polyclonal anti-phospho-p70 S6 kinase (Thr389) (Cell Signaling Technology).
Intermittent hypoxia treatment (IHT)
Mice underwent various protocols of IHT: IH5D, IH5DR2, and IH28D (Fig. 1c). Mice in the IH5D group were exposed to the IHT for 5 times, and then sacrificed immediately after the end of the final IHT. Mice in the IH5DR2 group were exposed to IHT for 5 times and followed by normoxia treatment for 64 h (16 h + 24 h × 2), and were then sacrificed. Mice in the IH28D group were exposed to the IHT for 28 times, and then sacrificed immediately after the end of the final IHT.
We applied the IH5D protocol as a standard to investigate the effect of IHT. The effects of rescue from IHT were investigated by exposing mice to the IH5DR2 protocol. The effects of elongating the IHT period were studied by exposing mice to the IH28D protocol. As the experimental control for the IH28D group, we reared mice for 28 days under normoxia condition (Ctrl28).
Immediately following the indicated protocol, mice were sacrificed by cervical dislocation according to procedures approved by the Animal Care and Use Committee of Saitama Medical University. We performed sacrifices and dissection in separate rooms. Isolated brains were chilled with phosphate-buffered saline, and stored at −80 °C until use.
Total RNA was extracted from hippocampus using ISOGEN (Nippon Gene, Tokyo, Japan), and was used for microarray analyses.
Microarray analysis was performed as described previously . Briefly, genome-wide mRNA expression profiles were obtained by microarray analysis with the Affymetrix GeneChip Mouse (430 2.0) Array, according to the manufacturer’s instructions. Normalized data are available at the GEO database (http://www.ncbi.nlm.nih.gov/geo/) under accession number GSE62385.
The microarray data obtained from the in vivo model and GEO (GSE32583 and GSE32591… etc.) were examined and visualized using R/Bioconductor , as described previously [25, 28]. The expression levels of our microarray data were calculated from probe intensities and arrays normalized using the mas5 method . Only probe sets with normalized signals >20 were defined as expressed, and were selected for analyses. The expression of individual genes was defined as altered when comparison of the average normalized signal intensities using the Bioconductor package Genefilter gave a value with P <0.05 using Welch’s ANOVA. We performed principle component analysis on the results of standardized microarray data using the statistical package R. In order to investigate the ontology of differentially expressed genes, enriched expression of GO terms were assessed and confirmed with the hypergeometric test , using the Bioconductor package GOstats/GSEABase. These programs determine which GO terms identified from lists of affected genes are statistically over— or under-represented, compared to the GO terms represented in the microarray as a whole. Hierarchical clustering was performed using Ward’s method to calculate the linkage distances based on the correlation coefficient between samples or GO terms.
Hippocampal samples were homogenized with Tris-buffered saline (TBS) containing protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany) and phosphatase inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). The homogenates were ultracentrifuged at 32,000 x g, for 20 min, at 4 °C (Rotor: TLA45, Beckman Coulter). Protein concentrations of the supernatants and the sonicated pellets were determined using a BCA protein assay kit (Nacalai Tesque), and were adjusted to 1 ~ 2 μg/μL with sample buffer containing SDS. Samples were boiled at 96 °C for 10 min before being used for conventional SDS-PAGE, followed by transfer to nitrocellulose membranes. After blocking with skim milk (MEGMILK SNOW BRAND Co., Ltd., Tokyo, Japan), the membranes were incubated with the indicated primary antibody. After treatment with horseradish peroxidase-conjugated secondary antibody, the membranes were treated with reagent for exposure (Chemi-Lumi One Super, Nacalai Tesque; ImmunoStar LD, Wako, Osaka, Japan). Images of the membranes were captured with the ChemiDocTM XRS+ system (Bio-Rad, Hercules, CA) or a C-Digit Blot Scanner (LI-COR, Lincoln, NE), and analyzed using Image J software.
The Y maze apparatus (Hazai-ya, Tokyo, Japan) was a 3-arm maze with equal angles between all arms (8 cm width) and a bottom with 40 cm (length) and 15 cm height. Mice were tested individually by placing them in an arm of the maze and allowing them to move freely throughout the 3 different arms for 10 min. The sequence and entries into each arm were recorded. An alternation was determined from successive consecutive entries into the 3 different arms on overlapping triads in which all arms were represented. For example, ACBABCABAB, a sequence of entries to the 3 arms A, B, or C, would generate 5 ‘successful’ alternations, ACB, CBA, ABC, BCA, and CAB; the total number of possible alternations corresponded to the number of the total arm entries minus 2 (in this example, the total number would equal 8). The percentage alternation was calculated as (the number of ‘successful’ alternations divided by the number of the total arm entries minus 2) × 100. We analyzed the percentage alternation and the total number of arm entries. The schedule of behavioral tests is shown in Fig. 5a or d. Mice completed the Y-maze test (Pre) and were then divided into control (Ctrl) and IHT groups. Significant differences between the 2 groups did not occur for the percentage alternation or the total number of arm entries (Figs. 5b and c, Pre; 5e-f, Pre). For the IH5D protocol (Fig. 5a), the IHT group was exposed to IHT for 5 days. For the remaining 2 days, all mice were treated with normoxia. At day 5 and 7, all mice completed the Y-maze test. For the IH28D protocol (Fig. 5d), the IHT group was exposed to IHT for 28 days. At day 28, all mice completed the Y-maze test.
Statistical analyses were performed using Prism 6 (GraphPad Software Inc., La Jolla CA). Quantification of Western blotting data were analyzed by one-way ANOVA followed by Dunnett’s tests. We used Student’s t-tests to compare 2 groups. Analyses of behavioral data were performed by two-way ANOVA followed by Tukey’s multiple comparison tests. Statistical significance was indicated by p <0.05.
GO-based microarray analysis reveals common biological processes between IHT and aging
In order to understand the effects of IHT on hippocampal functioning, mice underwent various IHT protocols: IH5D, IH5DR2, and IH28D (Fig. 1; also see the Methods section). As the experimental control for IH28D, we used mice reared for 28 days under normoxia condition (Ctrl28). Since a previous review indicated that IHT caused aging processes , we also studied mice reared for 12 months (12 m) at our institution. Total hippocampal RNA was extracted, and used for the microarray analysis.
Simultaneously, we performed a GO-based microarray analysis to compare our experimental data (GSE62385) with the various data obtained from GEO: hippocampal microarray data that were relevant to memory formation, cognitive impairment, neurodegeneration, and development. Based on the GO-based microarray analysis, we generated a GO heatmap with a gene clustering dendrogram on the left, indicating linkage in alteration profile. We also produced a sample correlation dendrogram on the bottom of the heatmap, indicating similarity in comprehensive gene expression among data from various experimental models (Additional file 1: Figure S1, the complete heatmap). Fig. 2c shows portions of the complete heatmap, which are marked with green rectangles in Figure S1. The dendrogram (Fig. 2c, bottom) revealed that similar alterations in gene expression patterns occurred with IHT (IH5D vs. Ctrl, IH5DR2 vs. Ctrl, IH28D vs. Ctrl28; GSE62385) and aging (12 m vs. Ctrl; GSE62385). Therefore, our PCA and GO-based microarray analyses indicate that IHT and aging shared common biological processes.
Summary of GO-based microarray analysis
GO term clusters
IH5D vs. Ctrl
IH28D vs. Ctrl28
12 m vs. Ctrl
18 m vs. 3.5 m
KA vs. Saline
Dicer KO vs. Ctrl
Dicer KO vs. Ctrl
Glud1 Tg vs. Ctrl
Moderate glutamate excess
The dendrogram (Fig. 2c, bottom; Additional file 1: Figure S1, bottom) also revealed that previous several experimental data had similar comprehensive gene expression to GSE62385 (IHT or aging) as follows: aging (18 months old vs. 3.5 months old) (GSE29075) , kainic acid (KA) treatment (KA-treated mice vs. Saline-treated mice) (GSE60772) , Dicer ablation (Dicer knock out (KO) mice vs. Ctrl) (GSE23847, GSE61937) [34, 35], and moderate glutamate excess (glutamate dehydrogenase 1 (Glud1) transgenic (Tg) mice vs. Ctrl) (GSE11419) [36, 37]. In particular, the alteration patterns of clusters A, B, C and E in these experimental models are summarized in Table 1. The left side of Fig. 2c displayed examples of data (GSE57583, GSE21137) [38, 39], in which the alteration patterns of GO terms differed from our experimental data (GSE62385).
IHT increased tau phosphorylation
In order to provide more functional information, we mapped and visualized genes that were altered by IHT or aging on the KEGG PATHWAY database. Previous studies have reported that the balances of kinases and/or phosphatases activities are altered during aging [40, 41]. Therefore, to determine whether imbalances of kinases and/or phosphatases occur with IHT or aging, we searched “MAPK signaling pathway” (map04010) (Additional file 1: Figure S2) and “PI3K-Akt signaling pathway” (map04151) (Additional file 1: Figure S3), since both pathways involve various kinases and phosphatases. The extent of alterations was amplified in the order corresponding to IH5D, and IH28D or aging, suggesting that IHT produces imbalances in kinases and/or phosphatases. In particular, we predicted the imbalance might direct towards an increase in kinase activities since the mapping on KEGG PATHWAY database mainly showed alterations in some kind of kinases.
In order to evaluate the imbalances of kinases and/or phosphatases, we focused on the microtubule-associated protein, tau. Tau has more than 40 potential phosphorylation sites, which are targets of various kinases and phosphatases [42, 43]. Altered levels of P-tau are therefore expected to reflect alterations in the balance of kinases and/or phosphatases. In addition, we found that IHT or aging, as well as Dicer KO models, demonstrate similar gene expression (Fig. 2c, the bottom dendrogram), particularly for alterations in several GO terms in cluster C (Fig. 2c; Table 1; Additional file 1: Figure S1). Furthermore, Dicer KO mice have exhibited increased P-tau [35, 44]. Therefore, we investigated whether IHT resulted in altered level of P-tau.
Next, we aimed to understand the mechanisms of altered P-tau. In particular, involvement of mammalian target of rapamycin (mTOR) was suggested using the map “PI3K-Akt signaling pathway” (map04151) in the KEGG PATHWAY database (Additional file 1: Figure S3). Previous reports have suggested that the mTOR signaling pathway may affect P-tau [45, 46]. In order to investigate the role of mTOR signaling in IHT-induced P-tau increases, we focused on p70 S6 kinase (p70S6K), glycogen synthase kinase-3β (GSK-3β), and protein phosphatase 2A (PP2A), which are direct or indirect P-tau regulators downstream of mTOR (Additional file 1: Figure S4) [46, 47]. Total GSK-3β and its phosphorylation at Ser9 and Tyr216 were comparable between the Ctrl, IH5D, and IH5DR2 groups (Fig. 3c, the top panels). PP2A is composed of subunits (i.e., PP2Aa, PP2Ab, and PP2Ac), and amounts of these PP2A subunits were comparable between the Ctrl, IH5D, and IH5DR2 groups (Fig. 3c, the middle panels). While total pS70S6K was comparable between the Ctrl, IH5D, and IH5DR2 groups, phosphorylated p70S6K increased significantly in the IH5D group (Fig. 3c, the bottom panels, Ctrl vs. IH5D). Normoxia treatment for 2 days reduced phosphorylated p70S6K to Ctrl levels (Fig. 3c, the bottom panels, Ctrl vs. IH5DR2). Increased phosphorylation of p70S6K indicates mTOR signaling pathway activation . Therefore, the results suggest that IHT increased P-tau via at least activation of mTOR signaling pathway. In contrast, the effects of IH5D were reversed by normoxia treatment.
Effect of IHT on behavior in Y-maze tests
Next, we performed Y-maze tests using mice exposed to the IH28D protocols. The tests were performed at day 0 (before any exposure; Pre), and day 28 (Fig. 5d). Percentage alternation results indicated that the IH28D group did not show significant impairments in working memory (Fig. 5e). The number of total entries still showed a tendency toward hyperactivity in the IH28D group, but the difference was not significant (Fig. 5f).
IH28D, but not IH5D, results in altered postsynaptic proteins
In summary, our in vivo observations (Figs. 3, 4, 5 and 6) are consistent with shared biological processes between IHT and aging (Fig. 2), thereby elucidating potential mechanisms of IHT-induced cognitive impairments that have been previously reported.
Common biological processes between IHT and aging may be related to AD
We firstly performed PCA and GO-based microarray analyses in order to elucidate the effects of IHT on hippocampal functioning. Although various studies have used microarray analyses so far, it is not easy to extract biological meanings from the analyses. In this study, we applied the previously-established GO-based microarray analysis  to the present study, and successfully found that IHT and aging shared alterations in some common GO, which were also observed with kainic acid treatment, Dicer ablation, or moderate glutamate excess. By performing in vivo analyses, we found that IHT increased P-tau via at least activation of mTOR signaling pathway, and decreased levels of postsynaptic proteins. These results confirmed the validity of our in silico analyses. The present study also support a previous suggestion that intermittent hypoxia is a cause of aging .
Aging is a major risk factor for AD . With aging, the brain may become susceptible to AD via amplification of molecular alterations that are qualitatively similar to normal aging [56, 57]. Given that IHT shared common biological processes to aging, it is possible that IHT also shared common biological processes to those involved in AD onset. During normal aging, and to a greater extent in tauopathies including AD, tau is phosphorylated to form neurofibrillary tangles (NFTs) in the brain [43, 57–62]. Our GO-based microarray analysis revealed that Dicer ablation, KA treatment, and a moderate glutamate excess produce similar gene expression patterns to IHT or aging (Fig. 2; Additional file 1: Figure S1). An increase in P-tau was observed in our IHT model (Figs. 3 and 4), and was also reported previously in Dicer KO mice  and KA-treated mice . Dicer ablation could may alter GO terms related to RNA metabolism, since Dicer is a type III RNase enzyme responsible for processing microRNA, which degrades or represses mRNA translation . Indeed, Dicer ablation alters several GO terms in cluster C that were related to RNA metabolism (Table 1; Fig. 2c; Additional file 1: Figure S1). Recent findings also indicate that alteration in microRNA metabolism may increase P-tau [65, 66], suggesting involvement of alterations in the balance of kinases and/or phosphatases. Alterations to clusters B and C also occur with KA treatment, and a moderate glutamate excess (Table 1). KA produces excitatory cell death , and Glud1 Tg mice exhibit neuronal loss in select brain regions, concurrent with indications of accelerated aging [36, 37]. In other words, both KA-treated mice and Glud1 Tg mice exhibit excessive neuronal excitation, which has been indicated as a common mechanism underlying aging or neurodegenerative diseases such as AD .
Accumulation of NFTs is correlated with neuronal loss and cognitive impairments in AD brains [48, 49]. These accumulations may also be responsible for memory loss associated with aging and memory deficits in some cases of mild cognitive impairment, which is an intermediate stage between aging and AD [50, 51]. Several mouse models expressing wild-type human tau have exhibited age-dependent accumulation of P-tau , which is associated with cognitive impairments [52, 53]. Some AD mouse models also show hyperactivity in Y-maze tests, which is ameliorated by reducing endogenous tau [69, 70]. These findings suggest that increased P-tau is closely relevant to cognitive impairments, including hyperactivity. Indeed, in the present study, hyperactivity in Y-maze tests was observed in the IH5D group (Fig. 5c). But, since the IH28D group did not exhibit a significant hyperactivity (Fig. 5f), the phenotype could be observed in acute phase of IHT rather than chronic phase. These insights suggested that biological processes associated with increment of P-tau are shared by IHT, aging, Dicer ablation, and KA treatment, and are also related to the onset of AD. Therefore, these may explain the mechanisms of the IHT-induced cognitive impairments that have been reported previously [16, 21–23].
We also found that IHT increased phosphorylated p70S6K, which is downstream of mTOR, and may regulate level of P-tau either directly or indirectly, such as through regulating activities of various kinases and/or phosphatases including GSK-3β and PP2A [45, 46] (Additional file 1: Figure S4). Thus, IHT-induced increases in P-tau may be partially explained by mTOR signaling pathway activation. Inhibition of mTOR by rapamycin and a genetically ablation of S6K increased lifespan [71, 72]. Activation of the mTOR signaling pathway has been suggested in relation to AD [24, 73]. Moreover, reducing S6K improved spatial memory and synaptic plasticity in an AD model . Therefore, IHT, aging, and AD may share common biological processes involving the mTOR signaling pathway.
We found that IH28D mice reduced postsynaptic proteins (GluA1, GluA2, PSD-95), but there were no changes in presynaptic proteins (VGluT1, synaptophysin). Our data are consistent with a previous study reporting that mice expressing human wild-type tau had reduced PSD-95, but that synaptophysin was not altered . Recently, it has been suggested that removal of GluA2 from the synapse is involved in the mechanisms of AD. Tau is localized in postsynapse, and is phosphorylated during the removal of GluA2 from synapse . This removal of GluA2 requires interaction between GluA2 and PICK1 , which is enhanced by the presence of tau . In the present study, IH28D mice exhibited a decrease in GluA2 and an increase in PICK1 to PSD-95 (Fig. 6), suggesting promotion of GluA2 removal.
These considerations confirmed that IHT and aging share common biological processes, which are also related to AD. However, the precise mechanism linking AD and aging is unknown, perhaps because there have been few models that reflect human-like pathology reflecting the effect of aging. IHT may mimic an environmental stimulus that contributes to AD progression, and may therefore be useful in producing feature that more closely models AD. Applying IHT to the existing AD models, such as an APP knock-in model , will therefore likely contribute to developing improved AD models that are desired for the future research .
IHT is a novel model to evaluate the physiological roles of P-tau
It still remains an enigma that the precise processes leading to increase in P-tau, as well as the effects of increased P-tau on neurons, the brain, and behavior. Further understanding will be enabled by generating animal models with increased endogenous P-tau, without using gene manipulation techniques. Previous studies have reported that non-transgenic mice exposed to starvation  or anesthesia  have increased endogenous P-tau. However, these previous models are not suitable for long-term observation or behavioral analyses. In contrast, IHT increased endogenous P-tau (Figs. 3 and 4), and it is possible to perform behavioral analyses on IHT model mice (Fig. 5). To the best of our knowledge, the IHT model is the first non-transgenic model that enables behavioral analyses under conditions of increased endogenous P-tau.
Using the IHT model, we found evidence for activation of the mTOR signaling pathway as a candidate mechanism of increased P-tau in vivo. In addition, our findings support that the increase in P-tau may contribute to hyperactivity in Y-maze tests, which is an AD model-related behavior. Therefore, the IHT model is suitable model for use in studying about the physiological roles of P-tau.
- FIO2 :
Fraction of inspired O2
Gene Expression Omnibus
Glutamate dehydrogenase 1
Glycogen synthase kinase-3β
Intermittent hypoxia treatment
Kyoto Encyclopedia of Genes and Genomes
Mammalian target of rapamycin
p70 S6 kinase
Principal component analysis
Protein phosphatase 2A
We thank to Mr. Naoya Umeda (Kyoto University) for in silico GO-based microarray analysis; Prof. Takenobu Katagiri (Saitama Medical University) for technical supports; Messrs. Kensuke Iwasa and Shinji Yamamoto (Saitama Medical University) for maintaining animals. We thank to Mses. Ayaka Sorimachi, Maya Moriizumi, Ayami Takahashi, Misaki Takahashi, and Mr. Kazuki Maeda (Saitama Medical University) for their technical supports. We also thank to staff of Division of Experimental Animal and of Division of Analytical Science (Biomedical Research Center, Saitama Medical University) for their continual supports.
This study was supported by Grant-in-Aid for Young Scientists (B) to S.Y. (23700429), Grant from Ochiai memorial award 2013 to S.Y., Research Fund of Mitsukoshi Health and Welfare Foundation 2013 to S.Y., Kurata Grant awarded by the Kurata Memorial Hitachi Science and Technology Foundation to S.Y., Grant-in-Aid for Research and Development Grants for Dementia from Japan Agency for Medical Research and Development (AMED) to S.Y. (16dk0207029h0001), Saitama Medical University Internal Grant 25-B-1-15 to M.S., and Grant-in-Aid for Scientific Research (C) to K.M. (25460341).
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its Additional files.
SY, SS, KY, KM, and TA designed the IHT protocols. SS, MS and TA made the apparatus for IHT. SY and SS performed the IHT experiments, and SY, SS, and AT performed biochemical analyses. KY constructed the environment for behavioral experiments in our institutes, and TA prepared the apparatus for the experiments. SY and SS performed behavioral analyses. SY, AH, and TA designed the microarray analyses. KI and AH performed microarray analyses, and the data were analyzed by AHirata and AH in silico. SY and AH wrote the manuscript, and AHirata read the proofs of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Peppard PE, Young T, Palta M, Skatrud J. Prospective study of the association between sleep-disordered breathing and hypertension. N Engl J Med. 2000;342:1378–84.View ArticlePubMedGoogle Scholar
- Nieto FJ, Young TB, Lind BK, Shahar E, Samet JM, Redline S, D’Agostino RB, Newman AB, Lebowitz MD, Pickering TG. Association of sleep-disordered breathing, sleep apnea, and hypertension in a large community-based study. Sleep heart health study. JAMA. 2000;283:1829–36.View ArticlePubMedGoogle Scholar
- Foley DJ, Monjan AA, Masaki KH, Enright PL, Quan SF, White LR. Associations of symptoms of sleep apnea with cardiovascular disease, cognitive impairment, and mortality among older Japanese-American men. J Am Geriatr Soc. 1999;47:524–8.View ArticlePubMedGoogle Scholar
- Shaw JE, Punjabi NM, Wilding JP, Alberti KG, Zimmet PZ, International Diabetes Federation Taskforce on E, Prevention. Sleep-disordered breathing and type 2 diabetes: a report from the international diabetes federation taskforce on epidemiology and prevention. Diabetes Res Clin Pract. 2008;81:2–12.View ArticlePubMedGoogle Scholar
- Ancoli-Israel S, Klauber MR, Butters N, Parker L, Kripke DF. Dementia in institutionalized elderly: relation to sleep apnea. J Am Geriatr Soc. 1991;39:258–63.View ArticlePubMedGoogle Scholar
- Osorio RS, Ayappa I, Mantua J, Gumb T, Varga A, Mooney AM, Burschtin OE, Taxin Z, During E, Spector N, et al. The interaction between sleep-disordered breathing and apolipoprotein E genotype on cerebrospinal fluid biomarkers for Alzheimer’s disease in cognitively normal elderly individuals. Neurobiol Aging. 2014;35:1318–24.View ArticlePubMedGoogle Scholar
- Ancoli-Israel S, Palmer BW, Cooke JR, Corey-Bloom J, Fiorentino L, Natarajan L, Liu L, Ayalon L, He F, Loredo JS. Cognitive effects of treating obstructive sleep apnea in Alzheimer’s disease: a randomized controlled study. J Am Geriatr Soc. 2008;56:2076–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Kotterba S, Rasche K, Widdig W, Duscha C, Blombach S, Schultze-Werninghaus G, Malin JP. Neuropsychological investigations and event-related potentials in obstructive sleep apnea syndrome before and during CPAP-therapy. J Neurol Sci. 1998;159:45–50.View ArticlePubMedGoogle Scholar
- Findley LJ, Barth JT, Powers DC, Wilhoit SC, Boyd DG, Suratt PM. Cognitive impairment in patients with obstructive sleep apnea and associated hypoxemia. Chest. 1986;90:686–90.View ArticlePubMedGoogle Scholar
- Yaffe K, Laffan AM, Harrison SL, Redline S, Spira AP, Ensrud KE, Ancoli-Israel S, Stone KL. Sleep-disordered breathing, hypoxia, and risk of mild cognitive impairment and dementia in older women. JAMA. 2011;306:613–9.PubMedPubMed CentralGoogle Scholar
- Smith SM, Friedle SA, Watters JJ. Chronic intermittent hypoxia exerts CNS region-specific effects on rat microglial inflammatory and TLR4 gene expression. PLoS One. 2013;8:e81584.View ArticlePubMedPubMed CentralGoogle Scholar
- Dayyat EA, Zhang SX, Wang Y, Cheng ZJ, Gozal D. Exogenous erythropoietin administration attenuates intermittent hypoxia-induced cognitive deficits in a murine model of sleep apnea. BMC Neurosci. 2012;13:77.View ArticlePubMedPubMed CentralGoogle Scholar
- Almado CE, Machado BH, Leao RM. Chronic intermittent hypoxia depresses afferent neurotransmission in NTS neurons by a reduction in the number of active synapses. J Neurosci. 2012;32:16736–46.View ArticlePubMedGoogle Scholar
- Coleman CG, Wang G, Park L, Anrather J, Delagrammatikas GJ, Chan J, Zhou J, Iadecola C, Pickel VM. Chronic intermittent hypoxia induces NMDA receptor-dependent plasticity and suppresses nitric oxide signaling in the mouse hypothalamic paraventricular nucleus. J Neurosci. 2010;30:12103–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Aviles-Reyes RX, Angelo MF, Villarreal A, Rios H, Lazarowski A, Ramos AJ. Intermittent hypoxia during sleep induces reactive gliosis and limited neuronal death in rats: implications for sleep apnea. J Neurochem. 2010;112:854–69.View ArticlePubMedGoogle Scholar
- Row BW, Kheirandish L, Cheng Y, Rowell PP, Gozal D. Impaired spatial working memory and altered choline acetyltransferase (CHAT) immunoreactivity and nicotinic receptor binding in rats exposed to intermittent hypoxia during sleep. Behav Brain Res. 2007;177:308–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Payne RS, Goldbart A, Gozal D, Schurr A. Effect of intermittent hypoxia on long-term potentiation in rat hippocampal slices. Brain Res. 2004;1029:195–9.View ArticlePubMedGoogle Scholar
- Row BW, Liu R, Xu W, Kheirandish L, Gozal D. Intermittent hypoxia is associated with oxidative stress and spatial learning deficits in the rat. Am J Respir Crit Care Med. 2003;167:1548–53.View ArticlePubMedGoogle Scholar
- Li RC, Row BW, Gozal E, Kheirandish L, Fan Q, Brittian KR, Guo SZ, Sachleben Jr LR, Gozal D. Cyclooxygenase 2 and intermittent hypoxia-induced spatial deficits in the rat. Am J Respir Crit Care Med. 2003;168:469–75.View ArticlePubMedGoogle Scholar
- Tagaito Y, Polotsky VY, Campen MJ, Wilson JA, Balbir A, Smith PL, Schwartz AR, O’Donnell CP. A model of sleep-disordered breathing in the C57BL/6J mouse. J Appl Physiol (1985). 2001;91:2758–66.Google Scholar
- Gozal D, Daniel JM, Dohanich GP. Behavioral and anatomical correlates of chronic episodic hypoxia during sleep in the rat. J Neurosci. 2001;21:2442–50.PubMedGoogle Scholar
- Xu W, Chi L, Row BW, Xu R, Ke Y, Xu B, Luo C, Kheirandish L, Gozal D, Liu R. Increased oxidative stress is associated with chronic intermittent hypoxia-mediated brain cortical neuronal cell apoptosis in a mouse model of sleep apnea. Neuroscience. 2004;126:313–23.View ArticlePubMedGoogle Scholar
- Li RC, Guo SZ, Raccurt M, Moudilou E, Morel G, Brittian KR, Gozal D. Exogenous growth hormone attenuates cognitive deficits induced by intermittent hypoxia in rats. Neuroscience. 2011;196:237–50.View ArticlePubMedPubMed CentralGoogle Scholar
- Bishop NA, Lu T, Yankner BA. Neural mechanisms of ageing and cognitive decline. Nature. 2010;464:529–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu N, Shi J, Xiao Y, Yasue M, Takei Y, Sanefuji H, Tsujimoto G, Hirasawa A. Effects of a tricaprylin emulsion on anti-glomerular basement membrane glomerulonephritis in rats: in vivo and in silico studies. Biol Pharm Bull. 2015;38:1175–84.View ArticlePubMedGoogle Scholar
- Takashima A, Murayama M, Murayama O, Kohno T, Honda T, Yasutake K, Nihonmatsu N, Mercken M, Yamaguchi H, Sugihara S, Wolozin B. Presenilin 1 associates with glycogen synthase kinase-3beta and its substrate tau. Proc Natl Acad Sci U S A. 1998;95:9637–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Ichimura A, Hirasawa A, Poulain-Godefroy O, Bonnefond A, Hara T, Yengo L, Kimura I, Leloire A, Liu N, Iida K, et al. Dysfunction of lipid sensor GPR120 leads to obesity in both mouse and human. Nature. 2012;483:350–4.View ArticlePubMedGoogle Scholar
- Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, et al. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004;5:R80.View ArticlePubMedPubMed CentralGoogle Scholar
- Hubbell E, Liu WM, Mei R. Robust estimators for expression analysis. Bioinformatics. 2002;18:1585–92.View ArticlePubMedGoogle Scholar
- Boyle EI, Weng S, Gollub J, Jin H, Botstein D, Cherry JM, Sherlock G. GO:: TermFinder--open source software for accessing gene ontology information and finding significantly enriched gene ontology terms associated with a list of genes. Bioinformatics. 2004;20:3710–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Di Giulio C. Is intermittent hypoxia a cause of aging? COPD. 2013;10:542–4.View ArticlePubMedGoogle Scholar
- Kohman RA, Rodriguez-Zas SL, Southey BR, Kelley KW, Dantzer R, Rhodes JS. Voluntary wheel running reverses age-induced changes in hippocampal gene expression. PLoS One. 2011;6:e22654.View ArticlePubMedPubMed CentralGoogle Scholar
- Kuzniewska B, Nader K, Dabrowski M, Kaczmarek L, Kalita K. Adult Deletion of SRF Increases Epileptogenesis and Decreases Activity-Induced Gene Expression. Mol Neurobiol. 2016;53:1478–1493.View ArticlePubMedGoogle Scholar
- Mang GM, Pradervand S, Du NH, Arpat AB, Preitner F, Wigger L, Gatfield D, Franken P. A neuron-specific deletion of the microRNA-processing enzyme DICER induces severe but transient obesity in mice. PLoS One. 2015;10:e0116760.View ArticlePubMedPubMed CentralGoogle Scholar
- Hebert SS, Papadopoulou AS, Smith P, Galas MC, Planel E, Silahtaroglu AN, Sergeant N, Buee L, De Strooper B. Genetic ablation of Dicer in adult forebrain neurons results in abnormal tau hyperphosphorylation and neurodegeneration. Hum Mol Genet. 2010;19:3959–69.View ArticlePubMedGoogle Scholar
- Wang X, Bao X, Pal R, Agbas A, Michaelis EK. Transcriptomic responses in mouse brain exposed to chronic excess of the neurotransmitter glutamate. BMC Genomics. 2010;11:360.View ArticlePubMedPubMed CentralGoogle Scholar
- Bao X, Pal R, Hascup KN, Wang Y, Wang WT, Xu W, Hui D, Agbas A, Wang X, Michaelis ML, et al. Transgenic expression of Glud1 (glutamate dehydrogenase 1) in neurons: in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability. J Neurosci. 2009;29:13929–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Wes PD, Easton A, Corradi J, Barten DM, Devidze N, DeCarr LB, Truong A, He A, Barrezueta NX, Polson C, et al. Tau overexpression impacts a neuroinflammation gene expression network perturbed in Alzheimer’s disease. PLoS One. 2014;9:e106050.View ArticlePubMedPubMed CentralGoogle Scholar
- Valor LM, Jancic D, Lujan R, Barco A. Ultrastructural and transcriptional profiling of neuropathological misregulation of CREB function. Cell Death Differ. 2010;17:1636–44.View ArticlePubMedGoogle Scholar
- Hsu KS, Huang CC, Liang YC, Wu HM, Chen YL, Lo SW, Ho WC. Alterations in the balance of protein kinase and phosphatase activities and age-related impairments of synaptic transmission and long-term potentiation. Hippocampus. 2002;12:787–802.View ArticlePubMedGoogle Scholar
- Norris CM, Halpain S, Foster TC. Alterations in the balance of protein kinase/phosphatase activities parallel reduced synaptic strength during aging. J Neurophysiol. 1998;80:1567–70.PubMedGoogle Scholar
- Stoothoff WH, Johnson GV. Tau phosphorylation: physiological and pathological consequences. Biochim Biophys Acta. 2005;1739:280–97.View ArticlePubMedGoogle Scholar
- Lee VM, Goedert M, Trojanowski JQ. Neurodegenerative tauopathies. Annu Rev Neurosci. 2001;24:1121–59.View ArticlePubMedGoogle Scholar
- Cheng S, Zhang C, Xu C, Wang L, Zou X, Chen G. Age-dependent neuron loss is associated with impaired adult neurogenesis in forebrain neuron-specific Dicer conditional knockout mice. Int J Biochem Cell Biol. 2014;57:186–96.View ArticlePubMedGoogle Scholar
- Caccamo A, Magri A, Medina DX, Wisely EV, Lopez-Aranda MF, Silva AJ, Oddo S. mTOR regulates tau phosphorylation and degradation: implications for Alzheimer’s disease and other tauopathies. Aging Cell. 2013;12:370–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Meske V, Albert F, Ohm TG. Coupling of mammalian target of rapamycin with phosphoinositide 3-kinase signaling pathway regulates protein phosphatase 2A- and glycogen synthase kinase-3 -dependent phosphorylation of Tau. J Biol Chem. 2008;283:100–9.View ArticlePubMedGoogle Scholar
- Pei JJ, Bjorkdahl C, Zhang H, Zhou X, Winblad B. p70 S6 kinase and tau in Alzheimer’s disease. J Alzheimers Dis. 2008;14:385–92.PubMedGoogle Scholar
- Gomez-Isla T, Hollister R, West H, Mui S, Growdon JH, Petersen RC, Parisi JE, Hyman BT. Neuronal loss correlates with but exceeds neurofibrillary tangles in Alzheimer’s disease. Ann Neurol. 1997;41:17–24.View ArticlePubMedGoogle Scholar
- Giannakopoulos P, Herrmann FR, Bussiere T, Bouras C, Kovari E, Perl DP, Morrison JH, Gold G, Hof PR. Tangle and neuron numbers, but not amyloid load, predict cognitive status in Alzheimer’s disease. Neurology. 2003;60:1495–500.View ArticlePubMedGoogle Scholar
- Guillozet AL, Weintraub S, Mash DC, Mesulam MM. Neurofibrillary tangles, amyloid, and memory in aging and mild cognitive impairment. Arch Neurol. 2003;60:729–36.View ArticlePubMedGoogle Scholar
- Mitchell TW, Mufson EJ, Schneider JA, Cochran EJ, Nissanov J, Han LY, Bienias JL, Lee VM, Trojanowski JQ, Bennett DA, Arnold SE. Parahippocampal tau pathology in healthy aging, mild cognitive impairment, and early Alzheimer’s disease. Ann Neurol. 2002;51:182–9.View ArticlePubMedGoogle Scholar
- Chabrier MA, Cheng D, Castello NA, Green KN, LaFerla FM. Synergistic effects of amyloid-beta and wild-type human tau on dendritic spine loss in a floxed double transgenic model of Alzheimer’s disease. Neurobiol Dis. 2014;64:107–17.View ArticlePubMedPubMed CentralGoogle Scholar
- Kimura T, Yamashita S, Fukuda T, Park JM, Murayama M, Mizoroki T, Yoshiike Y, Sahara N, Takashima A. Hyperphosphorylated tau in parahippocampal cortex impairs place learning in aged mice expressing wild-type human tau. EMBO J. 2007;26:5143–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang Q, Yu S, Simonyi A, Sun GY, Sun AY. Kainic acid-mediated excitotoxicity as a model for neurodegeneration. Mol Neurobiol. 2005;31:3–16.View ArticlePubMedGoogle Scholar
- Kawas C, Gray S, Brookmeyer R, Fozard J, Zonderman A. Age-specific incidence rates of Alzheimer’s disease: the Baltimore longitudinal study of aging. Neurology. 2000;54:2072–7.View ArticlePubMedGoogle Scholar
- Fjell AM, McEvoy L, Holland D, Dale AM, Walhovd KB. Alzheimer’s Disease Neuroimaging I: What is normal in normal aging? Effects of aging, amyloid and Alzheimer’s disease on the cerebral cortex and the hippocampus. Prog Neurobiol 2014;117:20–40.
- Mattson MP, Magnus T. Ageing and neuronal vulnerability. Nat Rev Neurosci. 2006;7:278–94.View ArticlePubMedPubMed CentralGoogle Scholar
- Wood JG, Mirra SS, Pollock NJ, Binder LI. Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau (tau). Proc Natl Acad Sci U S A. 1986;83:4040–3.View ArticlePubMedPubMed CentralGoogle Scholar
- Ihara Y, Nukina N, Miura R, Ogawara M. Phosphorylated tau protein is integrated into paired helical filaments in Alzheimer’s disease. J Biochem. 1986;99:1807–10.PubMedGoogle Scholar
- Grundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI. Abnormal phosphorylation of the microtubule-associated protein tau (tau) in Alzheimer cytoskeletal pathology. Proc Natl Acad Sci U S A. 1986;83:4913–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Elobeid A, Soininen H, Alafuzoff I. Hyperphosphorylated tau in young and middle-aged subjects. Acta Neuropathol. 2012;123:97–104.View ArticlePubMedGoogle Scholar
- Price JL, Morris JC. Tangles and plaques in nondemented aging and “preclinical” Alzheimer’s disease. Ann Neurol. 1999;45:358–68.View ArticlePubMedGoogle Scholar
- Liang Z, Liu F, Iqbal K, Grundke-Iqbal I, Gong CX. Dysregulation of tau phosphorylation in mouse brain during excitotoxic damage. J Alzheimers Dis. 2009;17:531–9.PubMedPubMed CentralGoogle Scholar
- Ambros V. The functions of animal microRNAs. Nature. 2004;431:350–5.View ArticlePubMedGoogle Scholar
- Banzhaf-Strathmann J, Benito E, May S, Arzberger T, Tahirovic S, Kretzschmar H, Fischer A, Edbauer D. MicroRNA-125b induces tau hyperphosphorylation and cognitive deficits in Alzheimer’s disease. EMBO J. 2014;33:1667–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Smith PY, Hernandez-Rapp J, Jolivette F, Lecours C, Bisht K, Goupil C, Dorval V, Parsi S, Morin F, Planel E, et al. miR-132/212 deficiency impairs tau metabolism and promotes pathological aggregation in vivo. Hum Mol Genet. 2015;24:6721–6735.
- Dong XX, Wang Y, Qin ZH. Molecular mechanisms of excitotoxicity and their relevance to pathogenesis of neurodegenerative diseases. Acta Pharmacol Sin. 2009;30:379–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Andorfer C, Kress Y, Espinoza M, de Silva R, Tucker KL, Barde YA, Duff K, Davies P. Hyperphosphorylation and aggregation of tau in mice expressing normal human tau isoforms. J Neurochem. 2003;86:582–90.View ArticlePubMedGoogle Scholar
- Harris JA, Devidze N, Verret L, Ho K, Halabisky B, Thwin MT, Kim D, Hamto P, Lo I, Yu GQ, et al. Transsynaptic progression of amyloid-beta-induced neuronal dysfunction within the entorhinal-hippocampal network. Neuron. 2010;68:428–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Roberson ED, Scearce-Levie K, Palop JJ, Yan F, Cheng IH, Wu T, Gerstein H, Yu GQ, Mucke L. Reducing endogenous tau ameliorates amyloid beta-induced deficits in an Alzheimer’s disease mouse model. Science. 2007;316:750–4.View ArticlePubMedGoogle Scholar
- Harrison DE, Strong R, Sharp ZD, Nelson JF, Astle CM, Flurkey K, Nadon NL, Wilkinson JE, Frenkel K, Carter CS, et al. Rapamycin fed late in life extends lifespan in genetically heterogeneous mice. Nature. 2009;460:392–5.PubMedPubMed CentralGoogle Scholar
- Selman C, Tullet JM, Wieser D, Irvine E, Lingard SJ, Choudhury AI, Claret M, Al-Qassab H, Carmignac D, Ramadani F, et al. Ribosomal protein S6 kinase 1 signaling regulates mammalian life span. Science. 2009;326:140–4.View ArticlePubMedPubMed CentralGoogle Scholar
- Pei JJ, Hugon J. mTOR-dependent signalling in Alzheimer’s disease. J Cell Mol Med. 2008;12:2525–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Caccamo A, Branca C, Talboom JS, Shaw DM, Turner D, Ma L, Messina A, Huang Z, Wu J, Oddo S. Reducing ribosomal protein S6 kinase 1 expression improves spatial memory and synaptic plasticity in a mouse model of Alzheimer’s disease. J Neurosci. 2015;35:14042–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Kimura T, Whitcomb DJ, Jo J, Regan P, Piers T, Heo S, Brown C, Hashikawa T, Murayama M, Seok H, et al. Microtubule-associated protein tau is essential for long-term depression in the hippocampus. Philos Trans R Soc Lond Ser B Biol Sci. 2014;369:20130144.View ArticleGoogle Scholar
- Hanley JG. PICK1: a multi-talented modulator of AMPA receptor trafficking. Pharmacol Ther. 2008;118:152–60.View ArticlePubMedGoogle Scholar
- Yagishita S, Murayama M, Ebihara T, Maruyama K, Takashima A. Glycogen synthase kinase 3beta-mediated phosphorylation in the most C-terminal region of protein interacting with C kinase 1 (PICK1) regulates the binding of PICK1 to glutamate receptor subunit GluA2. J Biol Chem. 2015;290:29438–48.View ArticlePubMedGoogle Scholar
- Saito T, Matsuba Y, Mihira N, Takano J, Nilsson P, Itohara S, Iwata N, Saido TC. Single app knock-in mouse models of Alzheimer’s disease. Nat Neurosci. 2014;17:661–3.View ArticlePubMedGoogle Scholar
- Medina M, Avila J. The need for better AD animal models. Front Pharmacol. 2014;5:227.View ArticlePubMedPubMed CentralGoogle Scholar
- Planel E, Miyasaka T, Launey T, Chui DH, Tanemura K, Sato S, Murayama O, Ishiguro K, Tatebayashi Y, Takashima A. Alterations in glucose metabolism induce hypothermia leading to tau hyperphosphorylation through differential inhibition of kinase and phosphatase activities: implications for Alzheimer’s disease. J Neurosci. 2004;24:2401–11.View ArticlePubMedGoogle Scholar
- Planel E, Richter KE, Nolan CE, Finley JE, Liu L, Wen Y, Krishnamurthy P, Herman M, Wang L, Schachter JB, et al. Anesthesia leads to tau hyperphosphorylation through inhibition of phosphatase activity by hypothermia. J Neurosci. 2007;27:3090–7.View ArticlePubMedGoogle Scholar