Figure 6
From: beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones
NgRs are dispensable for MAG-induced growth cone repulsion of hippocampal neurons. A. MAG-induced growth cone turning after the PI-PLC treatment. Primary hippocampal neurons were pre-treated with PI-PLC (1 unit/ml) for 30 min at 37°C and then growth cones were examined in a gradient of MAG. Shown is the summary of turning angles of axons with or without the PI-PLC treatment. B. MAG-induced growth cone turning of hippocampal neurons from NgR knockout mice and WT littermates. Shown is the summary of turning angles for axonal growth cones of P5 mouse hippocampal neurons derived from wild-type or NgR knockout (NgR KO) mice in a gradient of MAG (150 μg/ml in the pipette) with or without Ha2/5 (1.0 μg/ml) in the bath. Values represent mean ± s.e.m. Numbers associated with the bar graph indicate the number of growth cones analyzed. "*" indicates significant difference (p < 0.01, ANOVA).