Articles

Nanoscale organization of Nicastrin, the substrate receptor of the γ-secretase complex, as independent molecular domains

Alterations in the canonical processing of Amyloid Precursor Protein generate proteoforms that contribute to the onset of Alzheimer’s Disease. Modified composition of γ-secretase or mutations in its subunits h...

Authors: Shekhar Kedia, Kousik Mandal, Pallavi Rao Netrakanti, Mini Jose, Sangram S. Sisodia and Deepak Nair

Selectively increasing GHS-R1a expression in dCA1 excitatory/inhibitory neurons have opposite effects on memory encoding

Growth hormone secretagogue receptor 1a (GHS-R1a) is widely distributed in brain including the hippocampus. Studies have demonstrated the critical role of hippocampal ghrelin/GHS-R1a signaling in synaptic phys...

Authors: Nan Li, Na Li, Fenghua Xu, Ming Yu, Zichen Qiao and Yu Zhou

Neuronal excitatory-to-inhibitory balance is altered in cerebral organoid models of genetic neurological diseases

The neuro-physiological properties of individuals with genetic pre-disposition to neurological disorders are largely unknown. Here we aimed to explore these properties using cerebral organoids (COs) derived fr...

Authors: Simote T. Foliaki, Benjamin Schwarz, Bradley R. Groveman, Ryan O. Walters, Natalia C. Ferreira, Christina D. Orrù, Anna Smith, Aleksandar Wood, Olivia M. Schmit, Phoebe Freitag, Jue Yuan, Wenquan Zou, Catharine M. Bosio, James A. Carroll and Cathryn L. Haigh

Bisperoxovanadium promotes motor neuron survival and neuromuscular innervation in amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is the most common motor neuron (MN) disease, with no present cure. The progressive loss of MNs is the hallmark of ALS. We have previously shown the therapeutic effects of t...

Authors: Junmei Wang, Lydia Tierney, Ranjeet Mann, Thomas Lonsway and Chandler L. Walker

Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

The generation of mature synaptic structures using neurons differentiated from human-induced pluripotent stem cells (hiPSC-neurons) is expected to be applied to physiological studies of synapses in human cells...

Authors: Kazuyuki Togo, Hayato Fukusumi, Tomoko Shofuda, Hiroshi Ohnishi, Hiroyuki Yamazaki, Mariko Kato Hayashi, Nana Kawasaki, Nobuyuki Takei, Takanobu Nakazawa, Yumiko Saito, Kousuke Baba, Hitoshi Hashimoto, Yuko Sekino, Tomoaki Shirao, Hideki Mochizuki and Yonehiro Kanemura

S100B is selectively expressed by gray matter protoplasmic astrocytes and myelinating oligodendrocytes in the developing CNS

Studies on the development of central nervous system (CNS) primarily rely on the use of specific molecular markers for different types of neural cells. S100B is widely being used as a specific marker for astro...

Authors: Junqing Du, Min Yi, Fang Zhou, Wanjun He, Aifen Yang, Mengsheng Qiu and Hao Huang

Comparative metabolic profiling of posterior parietal cortex, amygdala, and hippocampus in conditioned fear memory

Fear conditioning and retrieval are suitable models to investigate the biological basis of various mental disorders. Hippocampus and amygdala neurons consolidate conditioned stimulus (CS)-dependent fear memory...

Authors: Yoonjeong Jeon, Yun Lim, Jiwoo Yeom and Eun-Kyoung Kim

Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents

The glutamatergic signaling pathway is involved in molecular learning and human cognitive ability. Specific single variants (SNVs, formerly single-nucleotide polymorphisms) in the genes encoding N-methyl-d-aspart...

Authors: Li-Ching Lee, Ming-Tsan Su, Hsing-Ying Huang, Ying-Chun Cho, Ting-Kuang Yeh and Chun-Yen Chang

Ackr3-Venus knock-in mouse lights up brain vasculature

The atypical chemokine receptor 3, ACKR3, is a G protein-coupled receptor, which does not couple to G proteins but recruits βarrestins. At present, ACKR3 is considered a target for cancer and cardiovascular di...

Authors: Aliza T. Ehrlich, Meriem Semache, Pierre Couvineau, Stefan Wojcik, Hiroyuki Kobayashi, Marcus Thelen, Florence Gross, Mireille Hogue, Christian Le Gouill, Emmanuel Darcq, Michel Bouvier and Brigitte L. Kieffer

Electroacupuncture improves repeated social defeat stress-elicited social avoidance and anxiety-like behaviors by reducing Lipocalin-2 in the hippocampus

Post-traumatic stress disorder (PTSD) is a trauma-related disorder that is associated with pro-inflammatory activation and neurobiological impairments in the brain and leads to a series of affective-like behav...

Authors: Yi-Hung Chen, Sheng-Yun Xie, Chao-Wei Chen and Dah-Yuu Lu

Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research

The Cre/LoxP-based conditional knockout technology is a powerful tool for gene function analysis that allows region- and time-specific gene manipulation. However, inserting a pair of LoxP cassettes to generate...

Authors: Hirofumi Nishizono, Yuki Hayano, Yoshihisa Nakahata, Yasuhito Ishigaki and Ryohei Yasuda

Hypoxia with inflammation and reperfusion alters membrane resistance by dynamically regulating voltage-gated potassium channels in hippocampal CA1 neurons

Hypoxia typically accompanies acute inflammatory responses in patients and animal models. However, a limited number of studies have examined the effect of hypoxia in combination with inflammation (Hypo-Inf) on...

Authors: Yoon-Sil Yang, Joon Ho Choi and Jong-Cheol Rah

Histone modification of pain-related gene expression in spinal cord neurons under a persistent postsurgical pain-like state by electrocautery

Chronic postsurgical pain (CPSP) is a serious problem. We developed a mouse model of CPSP induced by electrocautery and examined the mechanism of CPSP. In this mouse model, while both incision and electrocaute...

Authors: Yosuke Katsuda, Kenichi Tanaka, Tomohisa Mori, Michiko Narita, Hideyuki Takeshima, Takashige Kondo, Yoshiyuki Yamabe, Misa Matsufuji, Daisuke Sato, Yusuke Hamada, Keisuke Yamaguchi, Toshikazu Ushijima, Eiichi Inada, Naoko Kuzumaki, Masako Iseki and Minoru Narita

Splice-variant specific effects of a CACNA1H mutation associated with writer’s cramp

The CACNA1H gene encodes the α1 subunit of the low voltage-activated Ca_v3.2 T-type calcium channel, an important regulator of neuronal excitability. Alternative mRNA splicing can generate multiple channel variant...

Authors: Ivana A. Souza, Maria A. Gandini and Gerald W. Zamponi

Side-by-side comparison of the effects of Gq- and Gi-DREADD-mediated astrocyte modulation on intracellular calcium dynamics and synaptic plasticity in the hippocampal CA1

Astrocytes express a plethora of G protein-coupled receptors (GPCRs) that are crucial for shaping synaptic activity. Upon GPCR activation, astrocytes can respond with transient variations in intracellular Ca²⁺. I...

Authors: Yana Van Den Herrewegen, Thomas M. Sanderson, Surajit Sahu, Dimitri De Bundel, Zuner A. Bortolotto and Ilse Smolders

Endosomal traffic and glutamate synapse activity are increased in VPS35 D620N mutant knock-in mouse neurons, and resistant to LRRK2 kinase inhibition

Vacuolar protein sorting 35 (VPS35) regulates neurotransmitter receptor recycling from endosomes. A missense mutation (D620N) in VPS35 leads to autosomal-dominant, late-onset Parkinson’s disease. Here, we stud...

Authors: Chelsie A. Kadgien, Anusha Kamesh and Austen J. Milnerwood

The phosphorylation status of eukaryotic elongation factor-2 indicates neural activity in the brain

Assessment of neural activity in the specific brain area is critical for understanding the circuit mechanisms underlying altered brain function and behaviors. A number of immediate early genes (IEGs) that are ...

Authors: Sang Ho Yoon, Woo Seok Song, Sung Pyo Oh, Young Sook Kim and Myoung-Hwan Kim

Arginine vasopressin in the medial amygdala causes greater post-stress recruitment of hypothalamic vasopressin neurons

Arginine vasopressin (AVP) is expressed in both hypothalamic and extra-hypothalamic neurons. The expression and role of AVP exhibit remarkable divergence between these two neuronal populations. Polysynaptic pa...

Authors: Wen Han Tong, Samira Abdulai-Saiku and Ajai Vyas

Brain-derived neurotrophic factor produced long-term synaptic enhancement in the anterior cingulate cortex of adult mice

Previous studies have demonstrated that brain-derived neurotrophic factor (BDNF) is one of the diffusible messengers for enhancing synaptic transmission in the hippocampus. Less information is available about ...

Authors: Hui-Hui Miao, Zhuang Miao, Ji-Gang Pan, Xu-Hui Li and Min Zhuo

Anxiolytic effect of GABAergic neurons in the anterior cingulate cortex in a rat model of chronic inflammatory pain

Chronic pain easily leads to concomitant mood disorders, and the excitability of anterior cingulate cortex (ACC) pyramidal neurons (PNs) is involved in chronic pain-related anxiety. However, the mechanism by w...

Authors: Fang-bing Shao, Jun-fan Fang, Si-si Wang, Meng-ting Qiu, Dan-ning Xi, Xiao-ming Jin, Jing-gen Liu, Xiao-mei Shao, Zui Shen, Yi Liang, Jian-qiao Fang and Jun-ying Du

Minhee Analysis Package: an integrated software package for detection and management of spontaneous synaptic events

To understand the information encoded in a connection between the neurons, postsynaptic current (PSC) has been widely measured as a primary index of synaptic strength in the field of neurophysiology. Although ...

Authors: Yong Gyu Kim, Jae Jin Shin and Sang Jeong Kim

Multivalent electrostatic pi–cation interaction between synaptophysin and synapsin is responsible for the coacervation

We recently showed that synaptophysin (Syph) and synapsin (Syn) can induce liquid–liquid phase separation (LLPS) to cluster small synaptic-like microvesicles in living cells which are highly reminiscent of SV ...

Authors: Goeun Kim, Sang-Eun Lee, Seonyoung Jeong, Jeongkun Lee, Daehun Park and Sunghoe Chang

Functional organization of the midbrain periaqueductal gray for regulating aversive memory formation

Innately aversive experiences produce rapid defensive responses and powerful emotional memories. The midbrain periaqueductal gray (PAG) drives defensive behaviors through projections to brainstem motor control...

Authors: Li-Feng Yeh, Takaaki Ozawa and Joshua P. Johansen

Similarities of developmental gene expression changes in the brain between human and experimental animals: rhesus monkey, mouse, Zebrafish, and Drosophila

Experimental animals, such as non-human primates (NHPs), mice, Zebrafish, and Drosophila, are frequently employed as models to gain insights into human physiology and pathology. In developmental neuroscience a...

Authors: Ryuichi Nakajima, Hideo Hagihara and Tsuyoshi Miyakawa

GDF11 expressed in the adult brain negatively regulates hippocampal neurogenesis

Growth differentiation factor 11 (GDF11) is a transforming factor-β superfamily member that functions as a negative regulator of neurogenesis during embryonic development. However, when recombinant GDF11 (rGDF...

Authors: Brittany A. Mayweather, Sean M. Buchanan and Lee L. Rubin

Assessing the effect of interaction between C-reactive protein and gut microbiome on the risks of anxiety and depression

Cumulative evidence shows that gut microbiome can influence brain function and behavior via the inflammatory processes. However, the role of interaction between gut dysbiosis and C-reactive protein (CRP) in th...

Authors: Yujing Chen, Peilin Meng, Shiqiang Cheng, Yumeng Jia, Yan Wen, Xuena Yang, Yao Yao, Chuyu Pan, Chun’e Li, Huijie Zhang, Jingxi Zhang, Zhen Zhang and Feng Zhang

Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply

The medium-chain fatty acids octanoic acid (C8) and decanoic acid (C10) are gaining attention as beneficial brain fuels in several neurological disorders. The protective effects of C8 and C10 have been propose...

Authors: Jens V. Andersen, Emil W. Westi, Emil Jakobsen, Nerea Urruticoechea, Karin Borges and Blanca I. Aldana

Modeling PTEN overexpression-induced microcephaly in human brain organoids

The phosphatase and tensin homolog (PTEN) protein, encoded by the PTEN gene on chromosome 10, is a negative regulator of the phosphoinositide 3-kinase (PI3K) signaling pathway. Loss of PTEN has been linked to an ...

Authors: Navroop Dhaliwal, Wendy W.Y. Choi, Julien Muffat and Yun Li

Somatostatin contributes to long-term potentiation at excitatory synapses onto hippocampal somatostatinergic interneurons

Somatostatin-expressing interneurons (SOM-INs) are a major subpopulation of GABAergic cells in CA1 hippocampus that receive excitation from pyramidal cells (PCs), and, in turn, provide feedback inhibition onto...

Authors: Anne-Sophie Racine, François-Xavier Michon, Isabel Laplante and Jean-Claude Lacaille

Cirbp-PSD95 axis protects against hypobaric hypoxia-induced aberrant morphology of hippocampal dendritic spines and cognitive deficits

Hypobaric hypoxia (HH) is a typical characteristic of high altitude environment and causes a spectrum of pathophysiological effects, including headaches, gliovascular dysfunction and cognitive retardation. Her...

Authors: Yang Zhou, Huanyu Lu, Ying Liu, Zaihua Zhao, Qian Zhang, Chong Xue, Yuankang Zou, Zipeng Cao and Wenjing Luo

Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo

Machado-Joseph disease (MJD, also known as spinocerebellar ataxia type 3) is a fatal neurodegenerative disease that impairs control and coordination of movement. Here we tested whether treatment with the histo...

Authors: Maxinne Watchon, Luan Luu, Katherine J. Robinson, Kristy C. Yuan, Alana De Luca, Hannah J. Suddull, Madelaine C. Tym, Gilles J. Guillemin, Nicholas J. Cole, Garth A. Nicholson, Roger S. Chung, Albert Lee and Angela S. Laird

Correction to: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency

Authors: Makoto Asahina, Reiko Fujinawa, Hiroto Hirayama, Ryuichi Tozawa, Yasushi Kajii and Tadashi Suzuki

De novo SCN8A and inherited rare CACNA1H variants associated with severe developmental and epileptic encephalopathy

Developmental and epileptic encephalopathies (DEEs) are a group of severe epilepsies that are characterized by seizures and developmental delay. DEEs are primarily attributed to genetic causes and an increasin...

Authors: Robin N. Stringer, Bohumila Jurkovicova-Tarabova, Ivana A. Souza, Judy Ibrahim, Tomas Vacik, Waseem Mahmoud Fathalla, Jozef Hertecant, Gerald W. Zamponi, Lubica Lacinova and Norbert Weiss

Altered hippocampal transcriptome dynamics following sleep deprivation

Widespread sleep deprivation is a continuing public health problem in the United States and worldwide affecting adolescents and adults. Acute sleep deprivation results in decrements in spatial memory and cogni...

Authors: Marie E. Gaine, Ethan Bahl, Snehajyoti Chatterjee, Jacob J. Michaelson, Ted Abel and Lisa C. Lyons

TPEN attenuates amyloid-β_25–35-induced neuronal damage with changes in the electrophysiological properties of voltage-gated sodium and potassium channels

To understand the role of intracellular zinc ion (Zn²⁺) dysregulation in mediating age-related neurodegenerative changes, particularly neurotoxicity resulting from the generation of excessive neurotoxic amyloid-β...

Authors: Wen-bo Chen, Yu-xiang Wang, Hong-gang Wang, Di An, Dan Sun, Pan Li, Tao Zhang, Wan-ge Lu and Yan-qiang Liu

Inhibition of autophagy by CRMP2-derived peptide ST2-104 (R9-CBD3) via a CaMKKβ/AMPK/mTOR pathway contributes to ischemic postconditioning-induced neuroprotection against cerebral ischemia-reperfusion injury

Cerebral ischemia, a common cerebrovascular disease, is characterized by functional deficits and apoptotic cell death. Autophagy, a type of programmed cell death, plays critical roles in controlling neuronal d...

Authors: Yuan Yao, Yingshi Ji, Jinghong Ren, Huanyu Liu, Rajesh Khanna and Li Sun

Age-related increase in caveolin-1 expression facilitates cell-to-cell transmission of α-synuclein in neurons

Parkinson's disease (PD) is the second most prevalent neurodegenerative disease, with aging being considered the greatest risk factor for developing PD. Caveolin-1 (Cav-1) is known to participate in the aging ...

Authors: Tae-Young Ha, Yu Ree Choi, Hye Rin Noh, Seon-Heui Cha, Jae-Bong Kim and Sang Myun Park

Overexpression of LIMK1 in hippocampal excitatory neurons improves synaptic plasticity and social recognition memory in APP/PS1 mice

Accumulating evidence indicates that the actin regulator cofilin is overactivated in Alzheimer’s Disease (AD), but whether this abnormality contributes to synaptic and cognitive impairments in AD is unclear. I...

Authors: Haiwang Zhang, Youssif Ben Zablah, An Liu, Dongju Lee, Haorui Zhang, Yanghong Meng, Changxi Zhou, Xingde Liu, Yiming Wang and Zhengping Jia

Transcriptomic profiling of high- and low-spiking regions reveals novel epileptogenic mechanisms in focal cortical dysplasia type II patients

Focal cortical dysplasia (FCD) is a malformation of the cerebral cortex with poorly-defined epileptogenic zones (EZs), and poor surgical outcome in FCD is associated with inaccurate localization of the EZ. Hen...

Authors: Arpna Srivastava, Krishan Kumar, Jyotirmoy Banerjee, Manjari Tripathi, Vivek Dubey, Devina Sharma, Nitin Yadav, M. C. Sharma, Sanjeev Lalwani, Ramesh Doddamani, P. Sarat Chandra and Aparna Banerjee Dixit

The role m⁶A RNA methylation is CNS development and glioma pathogenesis

Epigenetic abnormalities play a crucial role in many tumors, including glioma. RNA methylation occurs as an epigenetic modification similar to DNA methylation and histone modification. m⁶A methylation is the most...

Authors: Ting Pan, Fan Wu, Liwen Li, Shiyan Wu, Fang Zhou, Ping Zhang, Caixing Sun and Liang Xia

RNA-seq co-expression network analysis reveals anxiolytic behavior of mice with Efnb2 knockout in parvalbumin+ neurons

Anxiety disorders are the most common psychiatric disorders, and the change in the activity of the prefrontal cortex (PFC) is considered as the underlying pathological mechanism. Parvalbumin-expressing (PV+) i...

Authors: Ying Sun, Le Ma, Jianhua Chen, Weidi Wang, Shiyu Peng, Ying Cheng, Yu Zhang, Jinghong Chen and Peijun Ju

Mutation of copper binding sites on cellular prion protein abolishes its inhibitory action on NMDA receptors in mouse hippocampal neurons

We have previously reported that cellular prion protein (PrP^C) can down-regulate NMDA receptor activity and in a copper dependent manner. Here, we employed AAV9 to introduce murine cellular prion protein into mou...

Authors: Sun Huang, Stefanie A. Black, Junting Huang, Peter K. Stys and Gerald W. Zamponi

Meningitic Escherichia coli α-hemolysin aggravates blood–brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling

Bacterial meningitis is a life-threatening infectious disease with severe neurological sequelae and a high mortality rate, in which Escherichia coli is one of the primary Gram-negative etiological bacteria. Menin...

Authors: Jiyang Fu, Liang Li, Dong Huo, Ruicheng Yang, Bo Yang, Bojie Xu, Xiaopei Yang, Menghong Dai, Chen Tan, Huanchun Chen and Xiangru Wang

Modeling temperature- and Cav3 subtype-dependent alterations in T-type calcium channel mediated burst firing

T-type calcium channels are important regulators of neuronal excitability. The mammalian brain expresses three T-type channel isoforms (Cav3.1, Cav3.2 and Cav3.3) with distinct biophysical properties that are ...

Authors: Fernando R. Fernandez, Mircea C. Iftinca, Gerald W. Zamponi and Ray W. Turner

Correction to: Developmental up-regulation of NMDA receptors in the prefrontal cortex and hippocampus of mGlu5 receptor knock-out mice

Authors: Tiziana Imbriglio, Remy Verhaeghe, Nico Antenucci, Stefania Maccari, Giuseppe Battaglia, Ferdinando Nicoletti and Milena Cannella

Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy

Clock genes not only regulate the circadian rhythm of physiological activities but also participate in the pathogenesis of many diseases. Previous studies have documented the abnormal expression of clock genes...

Authors: Hao Wu, Yong Liu, Lishuo Liu, Qiang Meng, Changwang Du, Kuo Li, Shan Dong, Yong Zhang, Huanfa Li and Hua Zhang

A role for BDNF- and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity

Memory and long term potentiation require de novo protein synthesis. A key regulator of this process is mTORC1, a complex comprising the mTOR kinase. Growth factors activate mTORC1 via a pathway involving PI3-...

Authors: Dany Khamsing, Solène Lebrun, Isabelle Fanget, Nathanaël Larochette, Christophe Tourain, Vincent de Sars, Maia Brunstein, Martin Oheim, Damien Carrel, François Darchen and Claire Desnos

Disruption of the astrocyte–neuron interaction is responsible for the impairments in learning and memory in 5XFAD mice: an Alzheimer’s disease animal model

The morphological dynamics of astrocytes are altered in the hippocampus during memory induction. Astrocyte–neuron interactions on synapses are called tripartite synapses. These control the synaptic function in...

Authors: Moonseok Choi, Sang-Min Lee, Dongsoo Kim, Heh-In Im, Hye-Sun Kim and Yun Ha Jeong

APOE genotype dependent molecular abnormalities in the cerebrovasculature of Alzheimer’s disease and age-matched non-demented brains

Cerebrovascular dysfunction is a hallmark feature of Alzheimer's disease (AD). One of the greatest risk factors for AD is the apolipoprotein E4 (E4) allele. The APOE4 genotype has been shown to negatively impa...

Authors: Joseph O. Ojo, Jon M. Reed, Gogce Crynen, Prashanthi Vallabhaneni, James Evans, Benjamin Shackleton, Maximillian Eisenbaum, Charis Ringland, Anastasia Edsell, Michael Mullan, Fiona Crawford and Corbin Bachmeier

Correction to: Pharmacological inhibition of nSMase2 reduces brain exosome release and α-synuclein pathology in a Parkinson’s disease model

Authors: Chunni Zhu, Tina Bilousova, Samantha Focht, Michael Jun, Chris Jean Elias, Mikhail Melnik, Sujyoti Chandra, Jesus Campagna, Whitaker Cohn, Asa Hatami, Patricia Spilman, Karen Hoppens Gylys and Varghese John