Figure 2
From: Lysosomal enzyme cathepsin D protects against alpha-synuclein aggregation and toxicity
α-syn accumulation does not co-localize with LC3 or CB immunoreactivity and is independent of active caspase-3 immunoreactivity. a. Immunostaining with Atg8/LC3 and α-syn antibodies shows that LC3 staining is increased in Ctsd-/- mice compared to Ctsd+/+ mice, and intense α-syn immunoreactivity does not colocalize with LC3 immunoreactivity (arrows, p25). LC3 = FITC (green). α-syn = Cy3 (red). Arrowheads point to cells with high LC3 immunoreactivity but without intense α-syn immunostaining. b. α-syn accumulation does not overlap with CB immunoreactivity. CB = FITC (green). α-syn = Cy3 (red). Arrow points to α-syn intense immunoreactive staining adjacent to CB staining. c. Neurons with active caspase-3 immunoreactivity do not exhibit intense α-syn immunoreactivity in Ctsd-/- brains. α-syn = FITC (green). Active caspase-3 = Cy3 (red). Arrows point to active caspase-3 immunoreactivity. Scale bar = 10 micron. n = 3 mice each genotype.