Figure 3

Immature DG of Alpha-CaMKII+/- Mice. (A) The number of BrdU-positive cells in the subgranular zone of the DG is increased in the alpha-CaMKII +/- mice at 7 weeks old, as observed 1 d after BrdU administration. The number of BrdU-positive cells within the DG was 52.8% higher (p = 0.00001) in the alpha-CaMKII +/- mice than in the wild-type mice. (B) In alpha-CaMKII+/- mice, the number of cells expressing calbindin (a mature-neuron marker) was decreased. (C) In alpha-CaMKII+/- mice, the number of cells expressing PSA-NCAM (a late-progenitor and immature-neuron marker) and calretinin (an immature-neuron marker) was markedly increased. CaMKII was co-expressed in a subset of PSA-NCAM-, calretinin-, and calbindin-expressing cells, indicated by the white arrowheads. For the analysis of expression of markers related to neurogenesis and differentiation, at least five mice were examined in each group. (D) Rapid Golgi staining showed that the number of Golgi-impregnated cells was decreased in the DG of alpha-CaMKII+/- mice (see Additional file 1, Figure S9) and that Golgi-impregnated cells in the DG of alpha-CaMKII+/- mice had less branching and shorter dendrites. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Error bars indicate s.e.m.