Figure 5
![Figure 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1756-6606-1-9/MediaObjects/13041_2008_Article_9_Fig5_HTML.jpg)
NR2B-NMDAR mediated Ca2+ influx under spiking-timing and pairing protocols. A. A representative image showing the CA1 pyramidal neuron filled with OGB-1. ROI, region of interests. B. Raw sample fluorescence images from the ROI before (1), during (2) and after (3) induction of LTP with the spike-timing protocol. Scale bar, 1.0 μm. C. Elevation of Ca2+ signal in the ROI during induction of LTP with the spike-timing protocol (upper). Treatment with ifenprodil (3 μM) reduced the Ca2+ signal (middle). The difference of [Ca2+] signals in control and in the presence of ifenprodil (lower) shows the NR2B-NMDAR mediated Ca2+ influx. D. Elevation of Ca2+ signal in the ROI during induction of LTP with the pairing protocol (upper). Treatment with ifenprodil (3 μM) reduced the Ca2+ signal (middle). The difference of [Ca2+] signals in control and in the presence of ifenprodil (lower) shows the NR2B-NMDAR mediated Ca2+ influx.