Figure 5

CPE reserves PKC-mediated inhibition of DAT uptake and cell surface expression. A, Uptake of [³H] DA in HEK-293 cells after a 30-min incubation at 37°C with vehicle alone (uptake buffer) or 2 μM PMA. Values are the percentage of uptake activity compared with untreated cells ± SEM. Data were analyzed by ANOVA, followed by post hoc Student-Newman-Keuls test. **, P < 0.01 compared with the group cotransfected with DAT and pcDNA3; ##, P < 0.01 compared with the group cotransfected with DAT and CPE untreated with PMA, n = 3. B, Summarized data indicating effects of CPE on DAT membrane expression in cotransfected HEK-293 cells after 30-min incubation at 37°C with vehicle alone (uptake buffer) or 2 μM PMA. Columns show the means ± SEM of the ratios of colorimetric readings. Data were analyzed by ANOVA, followed by post hoc Student-Newman-Keuls test. **, P < 0.01 compared with the group cotransfected with DAT and pcDNA3; ##, P < 0.01 compared with the group cotransfected with DAT and CPE untreated with PMA, n = 3.