Figure 1

Consolidation of rotarod-running skill requires protein synthesis. Naïve rats were trained one trial each day for 3 consecutive days. Anisomycin or ACSF was infused bilaterally into the ventricle at 0, 2, 6 or 12 h after each trial, as indicated by the black arrows in A, B, C and D, respectively. Retention was tested on day 5 and 6, one trial each day. Data are expressed as means ± SEM. (A) Rats treated with anisomycin (ANI) 0 h post-trial showed a deficit in acquisition and retention of the rotarod-running skill. * p < 0.05 for ANI group (n = 9 rats) vs. ACSF group (n = 7 rats), unpaired t-test. (B) Rats treated with anisomycin (ANI) 2 h post-trial performed the rotarod-running task equally well with those treated with ACSF. ANI group, n = 10 rats; ACSF group, n = 9 rats. (C) Rats treated with anisomycin (ANI) at 6 h post-training showed a deficit in acquisition and retention of the rotarod-running skill. * p < 0.05, ** p < 0.01 for ANI group (n = 8 rats) vs. ACSF group (n = 10 rats), unpaired t-test. (D) Rats treated with anisomycin (ANI) 12 h post-trial showed a deficit in acquisition and retention of the rotarod-running skill. * p < 0.05, ** p < 0.01 for ANI group (n = 7 rats) vs. ACSF group (n = 8 rats), unpaired t-test.