Figure 3

Abnormal social behaviors in nNOS KO mice. (A-F) Social interaction test in a novel environment (one-chamber social interaction test): total duration of active contacts (A), mean duration of each contact (B), number of contacts (C), total distance traveled (D), and total contact duration (E) were recorded. The relationship between distance traveled and number of contacts was plotted (F). The p values indicate genotype effect in two-way ANOVA (A-E). (G-I) Social interaction test in home cage: mean number of particles detected (G) and activity level (H) were recorded over 6 days. The averaged graph over 3 days of mean number of particles detected (I) and activity level (J). The relationship between activity level and number of particles was plotted (K). The p values indicate genotype effect in two-way repeated measures ANOVA (G-J). (L-V) Crawley's three-chamber social approach test. In the sociability test (L-V), time spent around the empty cage or the cage containing a stranger (L, M) and time spent in the chamber with an empty cage or cage containing a stranger (N, O) were recorded. In the preference for social novelty test (P-S), time spent around the cage containing a stranger (stranger 2) and the cage with a familiar mouse (stranger1) (P, Q) and time spent in the chamber with the cage containing a stranger (stranger 2) and the cage with a familiar mouse (stranger1) cage (R, S) were recorded. Distance traveled was recorded in both tests (T, U). The ratio of distance traveled in the sociability test to that traveled in the preference for social novelty test is shown (V). The p values indicate the difference between the cages (stranger side vs. empty side; L-O, stranger 2 side vs. stranger 1 side; P-S). The p values indicate genotype effect in two-way ANOVA (T-V).