Figure 1

Aβ- and nicotine-induced activation of ERK in cultured hippocampal slices. Hippocampal slice cultures were treated with culture media, media containing Aβ (100 nM), or media containing nicotine (500 nM) for either 5 min or 10 min, respectively. Confocal images of hippocampal area CA1 were obtained from slices that were double labeled using antibodies specific for phospho-ERK (red) and A) the neuronal marker NeuN (green), B) the dendritic marker MAP2 (green), C) a marker for GABAergic interneurons GAD67 (green), D) the astrocytic marker GFAP (green), and E) the microglial marker IB4 (green). Dual labeling is indicated by yellow/orange. All the images were taken with a 20× objective and inserts represent higher magnifications (optical zoom 3.0). The scale bars are the same in all images and represent 20 μm and 10 μm, respectively.