Unitary IPSCs in ACC pyramidal neuron with high [Cl-]
. ( A ) Outward and inward uIPSCs in ACC pyramidal neurons with low [Cl-]i and high [Cl-]i, respectively. Left, diagram of the connection. Middle, interneuronal firing (upper) induced outward uIPSCs in the postsynaptic pyramidal neurons at a holding potential of 0 mV with low [Cl-]i (lower). Right, no current is induced if holding at of --70 mV with low [Cl-]i (upper), while an inward current is obtained at a holding potential of --70 mV if the same neuron is re-patched with high [Cl-]i intracellular solution (upper). ( B-D ) Pooled data (open circle and triangle) and summarized results (bar graph) indicate the rise time (B), decay time (C) and half-width (D) of uIPSCs obtained under low [Cl-]i and high [Cl-]i. Unitary IPSCs with high [Cl-]i have faster kinetics than those with low [Cl-]i.