Figure 2

CBP inhibits CLOCK/BMAL1 activity by targeting CLOCK in NIH3T3 cells. (A) Cells were transiently transfected with pGAL4RE-Luc (5 ng), together with either pGAL4 or pGAL4-BMAL1 (100 ng), alone or in combination with pcDNA3CBP (100 ng) alone, pcDNA3CLOCK (100 ng) alone, or pcDNA3CBP and pcDNA3CLOCK, as indicated. (B) Cells were transiently transfected with pGAL4RE-Luc (5 ng), together with either pGAL4 or pGAL4-CLOCK (100 ng) alone or in combination with pcDNA3CBP (100 ng) alone, pcDNA3BAML1 (100 ng) alone, or pcDNA3CBP and pcDNA3BMAL1, as indicated. (C) Summary of Fig. 2 (A) and (B). The ratios of the transcriptional activity of each group with and without co-expression of CBP are indicated. An asterisk indicates p < 0.05 (Student's t test). Empty vector (pcDNA3) was used to standardize the total amount of transfected DNA (405 ng).