Abnormalities in brain structure and behavior in GSK-3alpha mutant mice

Table 3 HPLC analysis of catecholamine neurotransmitters

	Dopaminergic		Norepinergic		Serotonergic
Monoamine neurotransmitters (ng/mg of tissue)	DA	HVA	NE	NM	5-HT	5-HIAA
Prefrontal Cortex
WT (n = 9)	0.041 ± 0.028	0.102 ± 0.006	0.48 ± 0.018	0.077 ± 0.003	0.426 ± 0.021	0.238 ± 0.01
KO (n = 9)	0.041 ± 0.018	0.086 ± 0.004*	0.47 ± 0.017	0.083 ± 0.006	0.394 ± 0.017	0.25 ± 0.007
Hippocampus
WT (n = 9)	0.018 ± 0.001	0.038 ± 0.003	0.651 ± 0.023	0.056 ± 0.003	0.669 ± 0.019	0.705 ± 0.042
KO (n = 9)	0.015 ± 0.002	0.041 ± 0.003	0.566 ± 0.016**	0.051 ± 0.004	0.625 ± 0.016	0.747 ± 0.029
Amygdala
WT (n = 9)	0.805 ± 0.041	0.303 ± 0.013	0.498 ± 0.018	0.055 ± 0.003	0.562 ± 0.021	0.466 ± 0.031
KO (n = 9)	0.835 ± 0.087	0.291 ± 0.012	0.506 ± 0.013	0.064 ± 0.003*	0.523 ± 0.021	0.492 ± 0.021
Striatum
WT (n = 9)	13.49 ± 0.42	1.486 ± 0.049	0.12 ± 0.009	0.029 ± 0.003	0.425 ± 0.023	0.407 ± 0.028
KO (n = 9)	13.486 ± 0.28	1.534 ± 0.036	0.117 ± 0.006	0.024 ± 0.002	0.42 ± 0.017	0.399 ± 0.01

Levels of monoamines and metabolites, including dopamine (DA), 4-hydroxy-3- methoxyphenylacetic acid (HVA), 3 norepinephrine (NE), normetanephrine (NM), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), were quantified by HPLC with electrochemical detection in prefrontal cortex (PFC), hippocampus (HIP), amygdala (AM) and striatum (STR) of GSK-3α KO (n = 9) and WT (n = 9) mice. In bold type *p.value ≤ 0.05; **p.value ≤ 0.01 KO vs. WT.

ISSN: 1756-6606