Figure 6From: Critical involvement of Rho GTPase activity in the efficient transplantation of neural stem cells into the injured spinal cordDifferential responses of NSPCs expressing mutated forms of Rho GTPases after withdrawal of bFGF. (A) Anti-activated caspase-3 immunostaining of cultured NSPCs infected with recombinant adenoviruses expressing mutated forms of Rho GTPases. Activated caspase-3 immunoreactivity was observed in a small fraction of NSPCs (red). Similar extent of adenovirus infection was confirmed by immunostaining with anti-β-galactosidase antibody (green; CA mutations) or anti-GFP antibody (green; DN mutations). Bar, 20 μm. (B) Quantification of cells immunopositive with anti-activated caspase-3. Percentage of immunopositive cells is presented. There was a significant increase of cell death in cells expressing RhoCA (GFP control; 0.9 ± 0.3%, CdcCA; 2.6 ± 0.5%, CdcDN; 1.5 ± 0.6%, RacCA; 1.9 ± 0.2%, RacDN; 0.5 ± 0.3%, RhoCA; 8.0 ± 1,1% (ANOVA, p < 0.01), RhoDN; 0.8 ± 0.3%).Back to article page