Figure 3

Alteration of cell types in mature RBP-J deficient retinae. Immunostaining (green) and in situ hybridization performed on P21 sections from Chx10Cre-RBP^f/fand wild-type retinae. (A-H) Compared to control retinae, both rhodopsin⁺ and Gnat1⁺ rod photoreceptor cells, and PNA⁺ and Arr3⁺ cone photoreceptor cells are significantly increased in RBP-J deficient retinae. (I-L) Brn3⁺ and Nefl⁺ ganglion cells are greatly decreased in RBP-J deficient retinae compared to wild-type. (M-T) Interneurons, including NF165⁺ horizontal cells (M, N), syntaxin⁺ amacrine cells (O, P), PKCα⁺ rod bipolar cells (Q, R), and NK3⁺ cone bipolar cells (S, T) are all decreased in RBP-J deficient retinae, relative to wild-type controls. (U-Z) Numbers of glutamine synthetase (GS)⁺ and clusterin⁺ Müller glial cells in Chx10Cre-RBP^f/fretinae are comparable to those in wild-type retinae, although GFAP⁺ endfeet of Müller glial cells are increased in the deficient retina. (A') Comparison of percentages of Rhodopsin⁺, PNA⁺, Brn3⁺, NF165⁺, syntaxin⁺, PKCα⁺, NK3⁺ and GS⁺ cells in the total population of retinal cells between Chx10Cre-RBP^f/fand wild-type retinae (*P < 0.05, **P < 0.01). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. Hoechst counterstaining is shown in magenta. Scale bar, 100 μm.