Figure 4
From: Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway
APP is Enriched in the Lysosome in Neuronal SN56 cells. SN56 cells were transiently co-transfected with fluorescent-tagged APP and compartment marker proteins and imaged using laser scanning confocal microscopy. A. SN56 cells were transiently co-transfected with the βAPP-CFP (shown in green) constructs along with compartment markers (red) as indicated demonstrating preferential co-localization of βAPP with lysosomal marker LAMP1. Colocalized pixels were identified as in figure 3 and displayed in the white colocalization channel. Scale Bar = 10 microns. B. The colocalization of the brightest 2% of pixels of APP and compartment markers was quantitated by Imaris software. Values are expressed as the mean ± SEM for a minimum of 50 cells each for Rab5, Rab7, Rab9 and LAMP1, and 20 cells for Cox8, drawn from at least 4 independent transfections. * indicates statistically significant difference from all other compartment markers (p < 0.05). ** indicates compartment markers that are statistically different from LAMP1 and Cox8, but not different from each other each other (p < 0.05).