Figure 3

More GFAP-enriched and hypertrophic astrocytes in A53T mice. (A-L') Representative images show GFAP staining (green) in the cerebral cortex-striatum (A-C'), brainstem (D-F'), spinal cord (G-I'), and substantia nigra pars compacta (SNpc) (J-L') of nTg mice (2.5-month old) and A53T mice at asymptomatic (1-month old, A53T 1M) and symptomatic (A53T*) stages. Hypertrophy of astrocytes is shown in the brainstem (F'), spinal cord (I'), and SNpc (L') of symptomatic A53T mice. The SNpc dopaminergic neurons were revealed by tyrosine hydroxylase (TH) staining (J-L, red). Scale bar: 200 μm (A-C, D-F, G-I), 50 μm (J-L), and 20 μm (A'-C', D'-F', G'-I', J'-L').