Figure 4

Inhibition of MAPK activity prevents the formation of ITM and LTM. A) U0126 treatment (0.2 ml injection of 500 μM) does not impair the ability to perform aerial respiration, as no significant difference in total breathing time was observed (ANOVA_2,9 F = 0.1741, p > 0.05, n = 10). B) ITM formation is obstructed by injection of U0125 1 hour prior to training (TS) (30' TS vs. 3 h MT p = 0.9201, n = 12). C) LTM formation is also blocked by injecting animals with U0126 prior to training (TS) (60' TS vs. 24 h MT p = 0.1630, n = 24).