Skip to main content
Figure 8 | Molecular Brain

Figure 8

From: Nigrostriatal neuronal death following chronic dichlorvos exposure: crosstalk between mitochondrial impairments, α synuclein aggregation, oxidative damage and behavioral changes

Figure 8

α-synuclein and ubiquitin protein aggregates in SN after chronic dichlorvos exposure. (A) The brain slides were probed with anti α -synuclein and ubiquitin antibody. The signals were revealed using HRP conjugated secondary antibody and photographed.(B)The brain slides were probed with anti α -synuclein and ubiquitin antibody. The signals were revealed using FITC conjugated secondary antibody and photographed. (C and D) The brain slides were probed with anti ubiquitin antibody. The signals were revealed using HRP conjugated secondary antibody and photographed. (E). Effect of chronic dichlorvos exposure on α -synuclein mRNA expression in SN and CS by RT-PCR.(F) Densitometric analysis α-synuclein/GAPDH.(G) Effect of chronic dichlorvos exposure on α-synuclein quantitative protein expression in SN and CS of rat brain. The proteins separated on 10% SDS-PAGE were subjected to immunoblotting using anti α-synuclein antibody. (H). Densitometric analysis blot α-synuclein/β actin. (I) Effect of chronic dichlorvos exposure on synaptophysin quantitative protein expression in substantia nigra and corpus striatum of rat brain. The proteins separated on 10% SDS-PAGE were subjected to immunoblotting using anti-synaptophysin antibody. (J) Densitometric analysis blot synaptophysin/β actin. Values are mean SD of 4 animals in each group, *p < 0.05 significantly different from control group. Lanes 1,3,5: Control; Lanes 2,4,6: Dichlorvos treated; CSN: control substantia nigra; DSN: Dichlorvos substantia nigra; CCS: Control corpus striatum; DCS: Dichlorvos corpus striatum; CHC: Control hippocampus; DHC: Dichlorvos hippocampus.

Back to article page

Molecular Brain

ISSN: 1756-6606