Figure 2

METH decreases BrdU incorporation and causes apoptosis in vitro. NPCs maintained under proliferative conditions were exposed to various concentrations of METH for 48 hours. BrdU (5 uM) was added for 4 hours to label proliferating cells. Cells were immunostained with antibody against BrdU and counterstained with DAPI. A. Quantitation of BrdU positive cells reveals a dose- and time-dependent decrease in BrdU positive cells upon exposure to METH (numbers following METH refer to concentration in µM). * p <0.01, ** p <0.001 ANOVA compared to control (proliferation conditions). B. Cells were stained with Trypan Blue and visualized under light microscopy. Numbers following M refer to concentration of METH in µM. * p <0.01, ** p <0.001. C. TUNEL positive cells were not detected in control cells, but were seen in some cells exposed for 48 hrs to METH 1000 µM (white arrows).